PureYield Plasmid Midiprep System from Promega

Product Specs
ItemPureYield Plasmid Midiprep System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA2495
Methodsilica-membrane column
Quantity100 preps
SourceBacterial
Nucleic Acid TypePlasmid DNA
Applicationspurification

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Occludin Regulates HIV-1 Infection by Modulation of the Interferon Stimulated OAS Gene FamilyMolecular neurobiologySeptember 1, 2023Torices S, Teglas T, Naranjo O, Fattakhov N, Frydlova K, Cabrera R, Osborne OM, Sun E, Kluttz A, Toborek M.Cat# C737303) and isolated using PureYield Plasmid midi-prep system (Promega, Madison, WI, USA, Cat# A2492). Viral stocks were created as described [15]. Briefly, a total of 50 μg of proviral plasmid was transfected

(2) Protocol to establish an oviduct epithelial cell line derived from Gallus gallus using Percoll for in vitro validation of recombinant proteinsSTAR ProtocAugust 4, 2023Chiron Loubser, Natalya V. Nikitinacabinet,Esco,Cat#2120759 Econo-Pac Chromatography Columns,Bio-Rad,Cat#7321010 PureYield Plasmid Midiprep System,Promega,Cat#A2492 10K MWCO Vivaspin 6 concentrator column,Sartorius,Cat#VS.0601 VWR Instructor’s Dissecting Kit,VWR,Cat#76457-530 Cohan-Vannas

(3) Modularity and diversity of target selectors in Tn7 transposonsMolecular CellJune 15, 2023Guilhem Faure, Makoto Saito, Sean Benler, Iris Peng, Yuri I. Wolf, Jonathan Strecker, Han Altae-Tran, Edwin Neumann, David Li, Kira S. Makarova, et al.Kit,Qiagen,28106 QIAprep Spin Miniprep Kit,Qiagen,27106 PureYield™ Plasmid Midiprep System,Promega,A2495 Gibson Assembly® Master Mix,New England Biolabs,E2611 NEBuilder® HiFi DNA Assembly Master Mix,New England Biolabs

(4) Programmable mammalian translational modulators by CRISPR-associated proteinsNature CommunicationsApril 19, 2023Shunsuke Kawasaki, Hiroki Ono, Moe Hirosawa, Takeru Kuwabara, Shunsuke Sumi, Suji Lee, Knut Woltjen, Hirohide SaitoTable 5. All Plasmids were purified using the Midiprep kit (QIAGEN, #12143/12145 or Promega, #A2492/A2495). Construction of IVT (in vitro transcription) template The ORF of SpCas9 (for trigger mRNA), 5’-UTR

(5) Protocol to measure centrosome cohesion deficits mediated by pathogenic LRRK2 in cultured cells using confocal microscopySTAR ProtocolsMarch 1, 2023Elena Fdez, Rachel Fasiczka, Antonio Jesús Lara Ordóñez, Belén Fernández, Yahaira Naaldijk, Sabine Hilfikerassays,Critical commercial assays,Critical commercial assays ,, PureYield Plasmid Midiprep System,Promega,Cat#A2492 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, "A549

(6) Temporal Notch signaling regulates mucociliary cell fates through Hes-mediated competitive de-repressionbioRxivFebruary 18, 2023Magdalena Maria Brislinger-Engelhardt, Fabian Lorenz, Maximilian Haas, Sarah Bowden, Alexia Tasca, Clemens Kreutz, Peter Walenteket al., 2013) was injected at 30ng/μl after purification using the PureYield Midiprep kit (Promega, #A2492). For antisense in situ hybridization probes, notch1, notch2, notch3, dlc, and jag2 fragments were cloned

(7) Occludin regulates HIV-1 infection by modulation of the interferon stimulated OAS gene familyResearch squareJanuary 30, 2023Torices S, Teglas T, Naranjo O, Fattakhov N, Frydlova K, Cabrera R, Osborne OM, Sun E, Kluttz A, Toborek M.Cat# C737303) and isolated using PureYield Plasmid midi-prep system (Promega, Madison, WI, USA, Cat# A2492). Viral stocks were created as described [16]. Briefly, a total of 50 μg of proviral plasmid was transfected

(8) PALI1 promotes tumor growth through competitive recruitment of PRC2 to G9A-target chromatin for dual epigenetic silencingMolecular cellDecember 15, 2022Fong KW, Zhao JC, Lu X, Kim J, Piunti A, Shilatifard A, Yu J.Kit,NEB,Cat#E7530S NEBNext® Ultra™ II DNA Library Prep Kit,NEB,Cat#E7645S PureYield Plasmid Midiprep System,Promega,Cat#A2495 LR clonase II,Life Tech,11791020 pCR®8/GW/TOPO® TA Cloning Kit,Life Tech,K250020 X-tremeGENE™ HP

(9) Systematic multi-omics cell line profiling uncovers principles of Ewing sarcoma fusion oncogene-mediated gene regulationCell ReportsDecember 6, 2022Martin F. Orth, Didier Surdez, Tobias Faehling, Anna C. Ehlers, Aruna Marchetto, Sandrine Grossetête, Richard Volckmann, Danny A. Zwijnenburg, Julia S. Gerke, Sakina Zaidi, et al.kit,"Macherey-Nagel, Düren, Germany",Cat#740952 PureYield Plasmid Midiprep System,"Promega, Maddison, WI, USA",Cat#A2492 Deposited data,Deposited data,Deposited data WGS data from 18 EwS cell lines; raw data,This paper,SRA:

(10) Mass spectrometry analysis of affinity-purified cytoplasmic translation initiation complexes from human and fly cellsSTAR ProtocolsDecember 1, 2022Chingakham Ranjit Singh, Naoki Tani, Akira Nakamura, Katsura Asanoassays,Critical commercial assays,Critical commercial assays ,, PureYieldTM Plasmid Midiprep System,Promega,A2492 Sliver stain II kit WAKO,Wako (Fisher Scientific),NC9535926 ,, Experimental models: Cell lines,Experimental models

(11) A noncanonical RNA-binding domain of the fragile X protein, FMRP, elicits translational repression independent of mRNA G-quadruplexesJournal of Biological ChemistryDecember 1, 2022MaKenzie R. Scarpitti, Julia E. Warrick, Evelyn L. Yoder, Michael G. Kearse# C404006), purified using the PureYield Plasmid Miniprep or Midiprep Systems (Promega # A1222 and A2495), and validated by Sanger sequencing at The Ohio State University Comprehensive Cancer Center Genomics

(12) Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systemsSTAR ProtocolsSeptember 16, 2022Zhiying Zhao, Jan-Fang Cheng, Yasuo Yoshikuniassays,Critical commercial assays ,, Gel extraction kit,Promega,Cat# A9282 Plasmid midi kit,Promega,Cat# A2495 Quant-it dsDNA HS assay kit,Invitrogen,Cat# Q32851 DNeasy blood & tissue Kits,QIAGEN,Cat# 69506 ,, Experimental models

(13) LRH ‐1/ NR5A2 interacts with the glucocorticoid receptor to regulate glucocorticoid resistanceEMBO reportsSeptember 5, 2022Svenja Michalek, Thomas Goj, Anna Pia Plazzo, Blerim Marovca, Beat Bornhauser, Thomas BrunnerHigh‐Fidelity PCR Kit,New England BioLabs,Cat# M0553 Pure Yield™ Plasmid Midiprep System,Promega,Cat# A2495 QIAquick Gel Extraction Kit,Qiagen,Cat# 28706X4 QIAquick PCR Purification Kit,Qiagen,Cat# 28104 Quick

(14) Pathogenic LRRK2 regulates centrosome cohesion via Rab10/RILPL1-mediated CDK5RAP2 displacementiScienceJune 17, 2022Fdez E, Madero-Pérez J, Lara Ordóñez AJ, Naaldijk Y, Fasiczka R, Aiastui A, Ruiz-Martínez J, López de Munain A, Cowley SA, Wade-Martins R, et al.assays ,, Gibson Assemby Master Mix,New England Biolabs,Cat#E2611S PureYield Plasmid Midiprep System,Promega,Cat#A2492 QuickChange II Site-Directed Mutagenesis Kit,Agilent Technologies,Cat#200523 Duolink In Situ PLA probe anti-rabbit PLUS

(15) A SARS-CoV-2 antibody broadly neutralizes SARS-related coronaviruses and variants by coordinated recognition of a virus-vulnerable siteImmunityOctober 12, 2021Onodera T, Kita S, Adachi Y, Moriyama S, Sato A, Nomura T, Sakakibara S, Inoue T, Tadokoro T, Anraku Y, et al.Minipreps DNA Purification Systems,Promega,Cat#A1460 PureYield Plasmid Midiprep System,Promega,Cat#A2495 R-Phycoerythrin Labeling Kit - NH2,DOJINDO,Cat#LK23 Allophycocyanin Labeling Kit - NH2,DOJINDO,Cat#LK21 QIAseq

(16) Decentralizing Cell-Free RNA Sensing With the Use of Low-Cost Cell ExtractsFrontiers in Bioengineering and BiotechnologyAugust 23, 2021Anibal Arce, Fernando Guzman Chavez, Chiara Gandini, Juan Puig, Tamara Matute, Jim Haseloff, Neil Dalchau, Jenny Molloy, Keith Pardee, Fernán Federiciwas produced by midi prepping an overnight culture of 200 ml LB with the appropriate strain (Promega, A2492) and cleaned again using PCR cleanup (Promega, A6754). Plasmid DNA inputs were used in a set of concentrations

(17) Dual modes of CRISPR-associated transposon homingCellApril 1, 2021Makoto Saito, Alim Ladha, Jonathan Strecker, Guilhem Faure, Edwin Neumann, Han Altae-Tran, Rhiannon K. Macrae, Feng ZhangKit,Qiagen,28106 QIAprep Spin Miniprep Kit,Qiagen,27106 PureYield™ Plasmid Midiprep System,Promega,A2495 Gibson Assembly® Master Mix,New England Biolabs,E2611 NEBuilder® HiFi DNA Assembly Master Mix,New England Biolabs

(18) Disabling VEGF-Response of Purkinje Cells by Downregulation of KDR via miRNA-204-5pInternational Journal of Molecular SciencesFebruary 22, 2021Julian Gehmeyr, Abdelouahid Maghnouj, Jonas Tjaden, Matthias Vorgerd, Stephan Hahn, Veronika Matschke, Verena Theis, Carsten Theissrpm and a midi-preparation was performed the subsequent day using PureYield Plasmid Midiprep System (A2492; Promega). The correct sequence was verified via standard sequencing and the resulting plasmid was stored

(19) Notch signaling induces either apoptosis or cell fate change in multiciliated cells during mucociliary tissue remodelingDevelopmental CellFebruary 1, 2021Alexia Tasca, Martin Helmstädter, Magdalena Maria Brislinger, Maximilian Haas, Brian Mitchell, Peter WalentekAssays,Critical Commercial Assays M-MLV reverse transcriptase,Promega,M3681 PureYield Midiprep kit,Promega,A2495 Ambion mMessage Machine kit,Thermo Fisher,AM1340 Q5 Site-directed mutagenesis kit,NEB,E0554S LysoTracker,Invitrogen,L7526

(20) Mutational resilience of antiviral restriction favors primate TRIM5Î± in host-virus evolutionary arms raceseLifeSeptember 15, 2020Jeannette L Tenthorey, Candice Young, Afeez Sodeinde, Michael Emerman, Harmit S Malik,NEBuilder HiFi DNA assembly cloning kit,NEB,E5520S,Gibson assembly kit Commercial assay or kit,PureYield plasmid miniprep kit,Promega,A2495,Transfection-quality plasmid miniprep (low LPS) Commercial assay or kit,NuceloBond Xtra midiprep kit,Takara

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5/18/2016Large High Quality Plasmid DNA Kit in Nominal Cost Sivaraman NatarajanOur lab regularly uses promega midi prep kit for large scale plasmid isolation from 100-200ml bacterial culture for transfection. We observed the quality of plamid DNA good enough for transfection. We... Read Review

PureYield™ Plasmid Midiprep System From Promega
Many protein expression procedures require large amounts of pure plasmid DNA. Over the years, a ... Read More
PureYield™ Plasmid Midiprep System from Promega
In addition to being very simple to use, all reagents can be stored at room temperature. So, no more... Read More