Enhanced K-Blue® Substrate (TMB) from Neogen Corporation

Product Specs
ItemEnhanced K-Blue® Substrate (TMB)
CompanyNeogen Corporation
Price Supplier Page View Company Product Page
Catalog Number308176
Regulatory StatusFor Research and Manufacturing Use Only
Quantity500 mL
ApplicationsColorimetric HRP Microwell
FormatReady to use

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Neogen Corporation

944 Nandino Boulevard

Lexington, Kentucky 40511

United States

Phone: 800-477-8201
859-254-1221

Company Profile

Website: https://www.neogen.com/industries/toxicology/

Website: https://www.neogen.com/solutions/life-science-research/

(1) Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available AntibodiesAnimals (Basel)November 4, 2022Paulina Krzysica, Loes Verhoog, Sonja de Vries, Coen Smits, Huub F. J. Savelkoul, Edwin TijhaarELISA plates were washed with the washing solution and tapped dry. Enhanced K-Blue TMB Substrate (#308177, Neogen, Lexington, KY, USA) (50 µL/well) was added to the plates and incubated for the appropriate

(2) An antagonistic monoclonal anti-Plexin-B1 antibody exerts therapeutic effects in mouse models of postmenopausal osteoporosis and multiple sclerosisThe Journal of biological chemistrySeptember 1, 2022Vogler M, Oleksy A, Schulze S, Fedorova M, Kojonazarov B, Nijjar S, Patel S, Jossi S, Sawmynaden K, Henry M, et al.between each incubation step with PBS-0.5% Tween at room temperature. Finally, K-Blue Substrate (Neogen, 308176) was added to all wells followed by Red Stop solution to stop the colorimetric reaction. Absorbance

(3) Immuno-proteomic profiling reveals aberrant immune cell regulation in the airways of individuals with ongoing post-COVID-19 respiratory diseaseImmunityMarch 8, 2022Vijayakumar B, Boustani K, Ogger PP, Papadaki A, Tonkin J, Orton CM, Ghai P, Suveizdyte K, Hewitt RJ, Desai SR, et al.2040-05/2050-05) for 1 hour at room temperature. Plates were developed with TMB substrate (Neogen, 308177) and reactions stopped with 0.18M sulfuric acid before measurement of absorbance at 450nm using a SpectraMax

(4) Balanced T and B cell responses are required for immune protection against Powassan virus in virus-like particle vaccinationCell ReportsFebruary 15, 2022E. Taylor Stone, Mariah Hassert, Elizabeth Geerling, Colleen Wagner, James D. Brien, Gregory D. Ebel, Alec J. Hirsch, Cody German, Jessica L. Smith, Amelia K. Pintotemperature. Plates were washed with ELISA wash buffer and 100 μL/well of enhanced TBD substrate (Neogen 308176) was allowed to incubate for 10–15 minutes during development of color change. Finally, 50μL/well of

(5) Monoclonal antibodies to Cache Valley virus for serological diagnosisPLOS Neglected Tropical DiseasesJanuary 24, 2022Benjamin Skinner, Sierra Mikula, Brent S. Davis, Jordan A. Powers, Holly R. Hughes, Amanda E. Calvertperiod of one hour at 37°C, plates were washed again 10 times. Enhanced K-blue TMB substrate (Cat# 308175, Neogen, Lansing, MI, USA) was added to each well of the plate (100 μL/well) and incubated in the dark

(6) A tetravalent live attenuated dengue virus vaccine stimulates balanced immunity to multiple serotypes in humansNature CommunicationsDecember 1, 2021Usha K. Nivarthi et al.plates were washed and 75 μL/well of the TMB substrate (enhanced K-Blue TMB substrate, Neogen Cat# 308175) was added. The plates were incubated at room temperature for 30 min or until the positive control turned

(7) Phenotypic microbial response to antimicrobial exposure conditions with a molecular analysis quantification of species-specific 16S rRNA contentMethodsXJuly 24, 2021Jade Chen, Michael Tomasek, Eliseo Nuñez, Vincent GauSwitzerland or Cat. No. RK1000-IDAST, GeneFluidics, Irwindale, CA-Enhanced K-Blue® Substrate (TMB), Cat. No. 308177, Neogen, Lansing, MI or Cat. No. RK1000-IDAST, GeneFluidics, Irwindale, CA-AST sensor chip, Cat. No.

(8) Production and Purification of Dengue Virus-like Particles from COS-1 CellsBIO-PROTOCOLJune 20, 2019Jedhan Ucat Galula, Gwong-Jen J. Chang, Day-Yu Chao115-035-062)3,3’,5,5’-tetramethylbenzidine (TMB) substrate (Neogen Corp., Enhanced K-Blue TMB, catalog number: 308177)Sulfuric acid (Sigma, catalog number: 30743-1L-GL)Bio-Rad protein assay dye reagent concentrate (Bio-Rad,

(9) Evaluation of pharmacokinetics and toxicology of biosimilar APZ001 antibody in Macaca cynomolgusTropical Journal of Pharmaceutical ResearchOctober 3, 2018Xiaofei Wang, Jianmin Guo, Xinyu Deng, Caiguo Ye, Yuankeng Huang, Xialing Lei, Huiqing Liang, Wei Yangsecondary antibody was purchased from Jackson (#109035-088) and TMB buffer was purchased from Neogen (#308176). Quantification of serum antibody concentration The serum concentration of cetuximab and APZ001 antibody

(10) Multiplexing of receptor occupancy measurements for pharmacodynamic biomarker assessment of biopharmaceuticalsCytometry Part B: Clinical CytometryMarch 1, 2016Inna Vainshtein, Amy K. Schneider, Bo Sun, Martin Schwickart, Lorin K. Roskos, Meina LiangSite. Goat anti‐human IgG‐(H+L)‐HRP conjugate (A80‐319P) was from Bethyl. Tetramethylbenzidine (TMB, 308175) was from Neogen. Streptavidin horseradish peroxidase (HRP, RPN4401) was from GE Healthcare. Cynomolgus monkey

(11) Fully human monoclonal antibody inhibitors of the neonatal fc receptor reduce circulating IgG in non-human primatesFrontiers in immunologyJanuary 1, 2015Nixon AE, Chen J, Sexton DJ, Muruganandam A, Bitonti AJ, Dumont J, Viswanathan M, Martik D, Wassaf D, Mezo A, et al.PBST. Reactions were developed with 100 μl/well TMB substrate (Neogen Labs Enhanced K-blue, Catalog # 308177) at room temperature in the dark (no plate shaking) for approximately 10 min. Reactions were stopped

(12) Patients with Encapsulating Peritoneal Sclerosis Have Increased Peritoneal Expression of Connective Tissue Growth Factor (CCN2), Transforming Growth Factor-β1, and Vascular Endothelial Growth FactorPLoS ONENovember 10, 2014Alferso C. Abrahams et al.Grove, PA, USA) was added, followed by horseradish peroxidase substrate (Enhanced K-Blue TMB substrate, 308175; Neogen Corp., Lexington, KY, USA). The color intensity was allowed to develop, and read at 650 nm using

(13) Gadolinium-induced fibrosis is counter-regulated by CCN3 in human dermal fibroblasts: a model for potential treatment of nephrogenic systemic fibrosisJournal of Cell Communication and SignalingJune 1, 2012Bruce L. Riser, Narasimharao Bhagavathula, Patricia Perone, Kendra Garchow, Yiru Xu, Gary J. Fisher, Feridoon Najmabadi, Durga Attili, James Varaniby more washing and the addition of horseradish peroxidase substrate (Enhanced K-Blue TMB substrate, 308175; Neogen Corp., Lexington, KY). The color intensity was allowed to develop before being read at 650 nm

(14) CCN3/CCN2 regulation and the fibrosis of diabetic renal diseaseJournal of Cell Communication and SignalingMarch 1, 2010Bruce L. Riser, Feridoon Najmabadi, Bernard Perbal, Jo Ann Rambow, Melisa L. Riser, Ernest Sukowski, Herman Yeger, Sarah C. Riser, Darryl R. Peterson(5′–3′) hCCN3 Sense Anti-sense Sense Anti-sense Sense Anti-sense Sense Anti-sense Sense Anti-sense Sense Anti-sense ATGCAGAGTGTGCAGAGCAC TTACATTTTCCCTCTGGTAGTCTTCA TCTGTGGGATCTGCAGTGAC ATTGTTCTGAGGGCAGTTGG GCACCAAGAAATCCCTGAAA GAGGGCAGTTGGAGTAGCAG AGTCTCTTCTGCGACTTCGG GCAACTGCTTTGGAAGGACT AGCAGCTGGGAGAACTGTGT TGGTATTTGCAGCTGCTTTG TGCTGCCTTTTCTGTTCCTT AAGGTGCTGGGTAGGGAAGT rCcn3 mCcn3 rCcn2 mCcn2 rCol1 42 strate, 308175, Neogen Corp., Lexington, KY). The color intensity was allowed to develop before being read at 650 nm

(15) CCN3 (NOV) is a negative regulator of CCN2 (CTGF) and a novel endogenous inhibitor of the fibrotic pathway in an in vitro model of renal diseaseThe American journal of pathologyMay 1, 2009Riser BL, Najmabadi F, Perbal B, Peterson DR, Rambow JA, Riser ML, Sukowski E, Yeger H, Riser SC.added, followed by more washing and horseradish peroxidase substrate (Enhanced K-␤lue TMB substrate, 308175; Neogen Corp., Lexington, KY). The color intensity was allowed to develop before being read at 650 nm

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