TMB Substrate Set from BioLegend

Product Specs
ItemTMB Substrate Set
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number421101
ApplicationsELISA
Quantity110 mL
TypeSOLUTION

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) An Animal Model for Chronic Meningeal Inflammation and Inflammatory Demyelination of the Cerebral CortexInternational journal of molecular sciencesSeptember 9, 2023Enz LS, Winkler A, Wrzos C, Dasen B, Nessler S, Stadelmann C, Schaeren-Wiemers N.blocking buffer, 405103, BioLegend). Thereafter, wells were washed, and 200 μL substrate solution (421101, BioLegend) was added per well. Upon sufficient color development, 100 μL stop solution (423001, BioLegend)

(2) Lung injury induces a polarized immune response by self-antigen-specific CD4+ Foxp3+ regulatory T cellsCell ReportsAugust 1, 2023Daniel S. Shin, Sneha Ratnapriya, Creel Ng Cashin, Lucy F. Kuhn, Rod A. Rahimi, Robert M. Anthony, James J. Moonrecombinant proteins","Chemicals, peptides, and recombinant proteins" ,, TMB Substrate Set,BioLegend,Cat#421101 Recombinant mouse IL-2,BioLegend,Cat#575402 2W peptide (EAWGALANWAVDSA),Genscript,N/A Aminoguanidine

(3) Tumor and local lymphoid tissue interaction determines prognosis in high-grade serous ovarian cancerCell Reports MedicineJuly 18, 2023Haonan Lu, Hantao Lou, Georg Wengert, Reema Paudel, Naina Patel, Saral Desai, Bill Crum, Kristofer Linton-Reid, Mitchell Chen, Dongyang Li, et al.anti-IgA: Thermo, PA1-74395; anti-GZMB: R&D, DY2906-05) and subsequently TMB substrates (Biolegend, 421101). After 15min incubation at room temperature, stop solution (0.16M H2SO4) was added and the absorbance

(4) Spatial dysregulation of T follicular helper cells impairs vaccine responses in agingNature ImmunologyJuly 1, 2023Alyssa Silva-Cayetano, Sigrid Fra-Bido, Philippe A. Robert, Silvia Innocentin, Alice R. Burton, Emily M. Watson, Jia Le Lee, Louise M. C. Webb, William S. Foster, Ross C. J. McKenzie, et al.Abcam) HRP-conjugated antibodies. The plates were developed with 100 µl per well of TMB solution (no. 421101, BioLegend) for up to 20 min, when the reaction was stopped with 50 µl per well of 0.5 M H2SO4. The

(5) The Src-Family Kinases SRC and BLK Contribute to the CLDN6-Adhesion SignalingCellsJune 23, 2023Naoki Ichikawa-Tomikawa, Kotaro Sugimoto, Korehito Kashiwagi, Hideki Chibawashes with TBS-T, the bound HRP was detected using 3,3′,5,5′-tetramethylbenzidine (TMB) substrate (421101, BioLegend, San Diego, CA, USA). The relative absorbance to the mean value of CLDN6C/GST was plotted

(6) Unmodified rabies mRNA vaccine elicits high cross-neutralizing antibody titers and diverse B cell memory responsesNature CommunicationsJune 22, 2023Fredrika Hellgren, Alberto Cagigi, Rodrigo Arcoverde Cerveira, Sebastian Ols, Theresa Kern, Ang Lin, Bengt Eriksson, Michael G. Dodds, Edith Jasny, Kim Schwendt, et al.cat# 246-GAMon/IgG(Fc)/PO, Nordic MUBio) followed by incubation with TMB substrate (BioLegend; cat# 421101) and stopped with a 1 M solution of H2SO4. Blocking and washing steps were used when appropriate. Absorbance

(7) Monoclonal antibodies against lipopolysaccharide protect against Pseudomonas aeruginosa challenge in miceFrontiers in Cellular and Infection MicrobiologyJune 22, 2023Jason Kang, Margalida Mateu-Borrás, Hunter L. Monroe, Emel Sen-Kilic, Sarah Jo Miller, Spencer R. Dublin, Annalisa B. Huckaby, Evita Yang, Gage M. Pyles, Mason A. Nunley, et al.After incubation, wells were washed five times with PBS-T and 100 µL of a TMB solution (BioLegend, #421101) was added to each well. After 10-15 min, the reaction was stopped by adding 50 µl of 2N sulfuric acid

(8) A linear B-cell epitope close to the furin cleavage site within the S1 domain of SARS-CoV-2 Spike protein discriminates the humoral immune response of nucleic acid- and protein-based vaccine cohortsFrontiers in ImmunologyMay 5, 2023Peter Lorenz, Felix Steinbeck, Franz Mai, Emil C. Reisinger, Brigitte Müller-Hilkesteps as described after the plasma sample incubation, the substrate reaction with TMB (BioLegend #421101) was initiated until the OD at 620nm (blue) of a chosen peptide/plasma sample used on each ELISA plate

(9) Delayed generation of functional virus-specific circulating T follicular helper cells correlates with severe COVID-19Nature communicationsApril 15, 2023Yu M, Charles A, Cagigi A, Christ W, Österberg B, Falck-Jones S, Azizmohammadi L, Åhlberg E, Falck-Jones R, Svensson J, et al.antibody from Thermo Fisher (cat no. A18781) followed by incubation with TMB substrate (BioLegend, cat no. 421101) which was stopped with a 1 M solution of H2SO4. Absorbance was read at 450 nm + 550 nm (background

(10) An epithelial cell-derived metabolite tunes immunoglobulin A secretion by gut-resident plasma cellsNature ImmunologyMarch 1, 2023Simona Ceglia, Alyssa Berthelette, Kelsey Howley, Yun Li, Benedikt Mortzfeld, Shakti K. Bhattarai, Nicole K. H. Yiew, Ying Xu, Robert Brink, Jason G. Cyster, et al.peroxidase (polyclonal, Southern Biotech) and visualized by the addition of TMB substrate set (Biolegend,#421101). Color development was stopped with 3 M of H2SO4 stop solution (Biolegend). Unlabeled mouse IgA (Southern Biotech

(11) Crosstalk between ILC2s and Th2 cells varies among mouse modelsCell reportsFebruary 28, 2023Gurram RK, Wei D, Yu Q, Butcher MJ, Chen X, Cui K, Hu G, Zheng M, Zhu X, Oh J, et al.washings with PBS buffer +0.05% Tween 20. The plates were developed with the TMB substrate solution (Cat# 421101; Biolegend). The stop solution for TMB substrate (Cat# 423001; Biolegend) was used to terminate the

(12) Urine biomarkers enable pancreatic cancer detection up to 2 years before diagnosisInternational journal of cancerFebruary 15, 2023Debernardi S, Blyuss O, Rycyk D, Srivastava K, Jeon CY, Cai H, Cai Q, Shu XO, Crnogorac-Jurcevic T.SEK11638; 1:500 urine dilutions) with TMB Substrate reagent and Stop Solution from BioLegend (Cat# 421101 and 423 001). Optical density was determined using the FLUOstar OMEGA Microplate Reader at 450 nm. Each

(13) Structural and Immunoreactivity Properties of the SARS-CoV-2 Spike Protein upon the Development of an Inactivated VaccineVirusesFebruary 9, 2023Larisa V. Kordyukova, Andrey V. Moiseenko, Marina V. Serebryakova, Marina A. Shuklina, Maria V. Sergeeva, Dmitry A. Lioznov, Andrei V. Shanko#A0170-1ML) was diluted at a ratio of 1:5000 and used as detecting antibodies, a TMB reagent (Biolegend 421101) was used as a substrate, and 2N H2SO4 was used as a stop solution. Absorbance was calculated by measuring

(14) The rapid and highly parallel identification of antibodies with defined biological activities by SLISYNature communicationsJanuary 3, 2023Lu S, Mattox AK, Aitana Azurmendi P, Christodoulou I, Wright KM, Popoli M, Chen Z, Sur S, Li Y, Bonifant CL, et al.1:10,000 in 1X TBST for 1 h at 4 °C. After 6 more 1X TBST washes, 100 µL of TMB substrate (Biolegend, 421101) was added and allowed to develop. The reaction was quenched with 50 µL of 2 N sulfuric acid. Absorbance

(15) Increased soluble HLA in COVID-19 present a disease-related, diverse immunopeptidome associated with T cell immunityiScienceDecember 22, 2022Annika Nelde, Jonas Rieth, Malte Roerden, Marissa L. Dubbelaar, Naomi Hoenisch Gravel, Jens Bauer, Reinhild Klein, Tobias Hoheisel, Hartmut Mahrhofer, Siri Göpel, et al.peptides, and recombinant proteins" ,, HLA monomer,Biolegend,Cat# 280301 TMB substrate,Biolegend,Cat# 421101 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, "Human HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DRA) ELISA kit

(16) CD47 restricts antiviral function of alveolar macrophages during influenza virus infectioniScienceDecember 22, 2022Wenzek C, Steinbach P, Wirsdörfer F, Sutter K, Boehme JD, Geffers R, Klopfleisch R, Bruder D, Jendrossek V, Buer J, et al.,R&D Systems,7859-TH-050 RNase-Free DNase Set,Qiagen,79254 ROTI Histofix,Roth,P087.3 TMB Substrate Solution,Biolegend,421101 Triton X-100,Roth,3051.4 Trypsin/EDTA,Sigma Aldrich,T4174 Trypsin from bovine prancreas,Sigma Aldrich,T8802 Tween 20

(17) Neutralization of the adaptor protein PAG by monoclonal antibody limits murine tumor growthMolecular Therapy - Methods & Clinical DevelopmentDecember 8, 2022Marianne Strazza, Emily K. Moore, Kieran Adam, Inbar Azoulay-Alfaguter, Adam Mortemperature. After the plate was washed, 3, 3′, 5, 5′ tetramethyl benzidine (TMB) substrate (catalog no. 421101; BioLegend) was added to each well. The reaction was stopped by adding 2N H2SO4. Absorbance was measured

(18) Omicron-specific mRNA vaccination alone and as a heterologous booster against SARS-CoV-2Nature CommunicationsDecember 1, 2022Zhenhao Fang et al.one hour. Plates were washed five times and developed with tetramethylbenzidine substrate (Biolegend, 421101). The reaction was stopped with 1 M phosphoric acid after 20 min at room temperature, and OD at 450

(19) Obesity and metabolic dysfunction drive sex-associated differential disease profiles in hACE2-mice challenged with SARS-CoV-2iScienceOctober 21, 2022Lee KS, Russ BP, Wong TY, Horspool AM, Winters MT, Barbier M, Bevere JR, Martinez I, Damron FH, Cyphert HA.nucleocapsid,Sino Biological,40588-V08B SARS-CoV-2 RBD,"Horspool et al., 2021",N/A TMB reagent,Biolegend,421101 Tween 20,Sigma-Aldrich,P1379-1L Dextrose (D-Glucose) Anhydrous,Fisher chemical,D16-500 ,, Critical

(20) B cell-intrinsic changes with age do not impact antibody-secreting cell formation but delay B cell participation in the germinal centre reactionAging cellSeptember 1, 2022Lee JL, Fra-Bido SC, Burton AR, Innocentin S, Hill DL, Linterman MA.#RPN1231V) for 30 min at room temperature. The plates were developed with 100 μl/well TMB (Biolegend #421101) for up to 20 min, and the reaction was stopped with 50 μl/well 2.5 M H2SO4. PHERAstar FS microplate

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TMB Substrate Set from BioLegend

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TMB Substrate Set from BioLegend

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