10-Acetyl-3,7-dihydroxyphenoxazine, which has been marketed under the name Amplex Red™ reagent, is regarded as the best fluorogenic substrate for peroxidase because it is highly specific and stable. The substrate itself is nearly colorless and nonfluorescent until it is oxidized by H2
in the presence of horseradish peroxidase (HRP) to become the highly red fluorescent resorufin. High purity resorufinis also available from Biotium to be used as a standard.
- lex\lem(pH 9)= 571/585 nm (end product)
- e= 54,000 (end product)
- White to off-white solid soluble in DMSO or DMF
- Store desiccated at 20 degrees C and protect from light
- Mwt: 257.24