WesternBright ECL Spray-HRP Substrate from Advansta

Product Specs
ItemWesternBright ECL Spray-HRP Substrate
CompanyAdvansta
Price Pricing Info Get Pricing
Catalog NumberK-12049-D50
Quantity500ml
ApplicationsWestern blotting
Brand/TrademarkAdvansta

Specifications/Features

WesternBright® ECL is more sensitive than other chemiluminescent substrates used for routine Western blots imaged using film, so you can conduct shorter exposures, load less sample, or use up to ten-times less antibody. Save time and money with WesternBright ECL. Features- Stronger signal than Amersham's ECL or Pierce's ECL. Detect low pg protein amounts. Use up to ten-times less antibody

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Advansta

2140 Bering Drive

San Jose, California 95131-2013

United States

Phone: 650.325.1980
650.325.1904

Company Profile

Website: http://advansta.com/

(1) MIF is a critical regulator of mononuclear phagocytic infiltration in hepatocellular carcinomaiScienceAugust 18, 2023Yunxi Liao, Chenyang Wu, Yang Li, Jinhua Wen, Dongyu Zhao,Thermo Fisher Scientific,Cat# EP0751 BCA Protein Assay Kit,Applygen,Cat# P1511 Enhanced Chemiluminescence Kit,Advansta,Cat# K-12049-D50 Human osteopontin ELISA Kit,Multisciences,Cat# 70-EK1135-48 Protease Inhibitor,Applygen,Cat# C1055 TRIzol® Reagent

(2) Polycomb repressive complex 2 binds and stabilizes NANOG to suppress differentiation-related genes to promote self-renewaliScienceJuly 21, 2023Yeh DW, Liu C, Hernandez JC, Tahara SM, Tsukamoto H, Machida K.,Thermo Fisher Scientific,A25776 SYBR™ Green PCR Master Mix,Thermo Fisher Scientific,4309155 WesternBright ECL-spray,Advansta,K-12049-D50 WesternBright ECL Quantum,Advansta,K-12042-D20 "Human Recombinant EZH2, GST tagged, full length",Creative BioMart

(3) Aurora kinase A/AURKA functionally interacts with the mitochondrial ATP synthase to regulate energy metabolism and cell deathCell Death DiscoveryJune 29, 2023Rakesh Kumar Sharma, Abderrahman Chafik, Giulia Bertolinone hour. They were again washed three times with TBS-T, and developed using westernBright ECL-spray (K-12049-D50, Advansta) and a CURIX 60 developer (Agfa Healthcare). Protein abundance was quantified using the Image

(4) Insight into the regulatory networks underlying the high lipid perennial ryegrass growth under different irradiancesPLOS ONEOctober 13, 2022Somrutai Winichayakul, Richard Macknight, Liam Le Lievre, Zac Beechey-Gradwell, Robyn Lee, Luke Cooney, Hong Xue, Tracey Crowther, Philip Anderson, Kim Richardson, et al.IgG-HRP (Sigma, A6154). Protein-antibody complexes were developed using the Western Bright ECL spray (K12049-D50, Advansta, CA) and visualized by the ChemiDoc™ MP Imaging system (Bio-Rad). Volume intensity of protein

(5) Caldesmon controls stress fiber force-balance through dynamic cross-linking of myosin II and actin-tropomyosin filamentsNature communicationsOctober 13, 2022Kokate SB, Ciuba K, Tran VD, Kumari R, Tojkander S, Engel U, Kogan K, Kumar S, Lappalainen P.RT. Finally, the blots were washed and protein bands were detected using WesternBright™ ECL-spray (K-12049-D50, Advansta) in the ChemiDoc XRS+ System (1708265, Biorad). Protein bands were quantified using Image

(6) An alternative angiosperm DGAT1 topology and potential motifs in the N-terminusFrontiers in Plant ScienceSeptember 16, 2022Somrutai Winichayakul, Amy Curran, Roger Moraga, Ruth Cookson, Hong Xue, Tracey Crowther, Marissa Roldan, Greg Bryan, Nick Roberts125-09481), 1:5000. Protein-antibody complexes were visualized using the Advansta Western Bright ECL spray (K12049-D50) and ChemiDoc MP Imager (Bio-Rad). In vitro cross-linking Microsomal protein (1 μg/μL) in PBS was

(7) Proglumide Reverses Nonalcoholic Steatohepatitis by Interaction with the Farnesoid X Receptor and Altering the MicrobiomeInternational journal of molecular sciencesFebruary 8, 2022Gay MD, Cao H, Shivapurkar N, Dakshanamurthy S, Kallakury B, Tucker RD, Kwagyan J, Smith JP.horseradish peroxidase, and developed by Western Bright ECL-Spray (Advansta, San Jose, CA, USA, Cat # K-12049-D50). The Spectra Multicolor Broad Range Protein Ladder (ThermoFisher, Waltham, MA, USA, Cat # 26634) was

(8) Circulating Exosomal miR-1290 for Diagnosis of Epithelial Ovarian CancerCurrent Issues in Molecular BiologyJanuary 9, 2022Hyeji Jeon et al.temperature. The signal was detected using ECL Western detection reagents (Advansta, San Jose, CA, USA, K-12049-D50) and a Molecular Images were captured by CheBi (Cellgentek, Deajeon, Chemi-luminescence Bioimaging Instrument

(9) Vaccination with Polyclonal Antibody Stimulator (PAS) Prevents Pancreatic Carcinogenesis in the KRAS Mouse ModelCancer prevention research (Philadelphia, Pa.)October 1, 2021Smith JP, Cao H, Chen W, Kallakury B, Phillips T, Sutton L, Cato A.IgG at RT for 1 hour, conjugated to HRP, and developed by WesternBright ECL-Spray (Advansta, cat # K-12049-D50). The Spectra Multicolor Broad Range Protein Ladder (ThermoFisher, cat # 26634) was used for molecular

(10) Structure of a Ty1 restriction factor reveals the molecular basis of transposition copy number controlNature communicationsSeptember 22, 2021Cottee MA, Beckwith SL, Letham SC, Kim SJ, Young GR, Stoye JP, Garfinkel DJ, Taylor IA.were detected with WesternBright enhanced chemiluminescence (ECL) detection reagent (Advansta cat. no. K-12049-D50). All imaging was done using a ChemiDoc MP (Bio-Rad). Precision Plus Kaleidoscope protein standards

(11) Astragaloside IV Suppresses Hepatic Proliferation in Regenerating Rat Liver after 70% Partial Hepatectomy via Down-Regulation of Cell Cycle Pathway and DNA ReplicationMoleculesMay 13, 2021Gyeong-Seok Lee, Hee-Yeon Jeong, Hyeon-Gung Yang, Young-Ran Seo, Eui-Gil Jung, Yong-Seok Lee, Kung-Woo Nam, Wan-Jong Kimby washing with PBS. Protein expression was detected by enhanced chemiluminescence (ECL) solution (K-12049-D50, Advansta Inc., San Jose, CA, USA) and chemiluminescence imaging system (GBox ichemi XL, Syngene, UK).

(12) Downstream neighbor of SON (DONSON) is associated with unfavorable survival across diverse cancers with oncogenic properties in clear cell renal cell carcinomaTranslational oncologyNovember 1, 2020Klümper N, Blajan I, Schmidt D, Kristiansen G, Toma M, Hölzel M, Ritter M, Ellinger J.Bio-Rad, Hercules, CA, USA). The chemiluminescence signal was depicted with the WesternBright ECL Spray (K-12049-D50, Advansta, San Jose, USA) and documented with a LAS 3000 Image Reader (Fujifilm, Tokyo, Japan). Immunohistochemistry DONSON

(13) Deficiency of GABARAP but not its Paralogs Causes Enhanced EGF-induced EGFR DegradationCellsMay 22, 2020Dobner J, Simons IM, Rufinatscha K, Hänsch S, Schwarten M, Weiergräber OH, Abdollahzadeh I, Gensch T, Bode JG, Hoffmann S, et al.fluorescent protein conjugated antibodies or after 2 min. incubation with Western Bright ECL spray (K-12049-D50, Advansta, San Jose, CA, USA) by chemiluminescence in case of HRP conjugated antibodies. The protein expression

(14) Pharmacological Activation of Pyruvate Kinase M2 Inhibits CD4⁺ T Cell Pathogenicity and Suppresses AutoimmunityCell metabolismFebruary 4, 2020Angiari S, Runtsch MC, Sutton CE, Palsson-McDermott EM, Kelly B, Rana N, Kane H, Papadopoulou G, Pearce EL, Mills KHG, et al.1203494-49-8 DSS,Thermo Scientific,Cat #: 21655; CAS: 68528-80-3 WesternBright ECL,Advansta,Cat #: K-12049-D50 Propidium Iodide,Invitrogen,Cat #: P1304MP; CAS: 25535-16-4 Pacific Blue™ Annexin V,BioLegend,Cat

(15) CHK1 dosage in germinal center B cells controls humoral immunityCell death and differentiationDecember 1, 2019Schoeler K, Jakic B, Heppke J, Soratroi C, Aufschnaiter A, Hermann-Kleiter N, Villunger A, Labi V.α-rat IgG/HRP (Cell Signaling 7077, 1:10,000). Secondary antibodies were detected using ECL (Advansta, K-12049-D50), and the chemiluminescent signal was visualized using X-ray ﬁlms (GE Healthcare 28906837; AGFA CURIX

(16) Checkpoint kinase 1 is essential for fetal and adult hematopoiesisEMBO reportsAugust 1, 2019Schuler F, Afreen S, Manzl C, Häcker G, Erlacher M, Villunger A.(Sigma‐Aldrich) and analyzed by Western blot analysis. For detection of proteins by chemoluminescence (Advansta, K‐12049‐D50), a mouse anti‐CHK1 (CS 2360, 2G1D5), rabbit anti‐ɣH2A.X (CS 2577), rabbit anti‐p53 (Santa Cruz sc‐6243),

(17) Checkpoint kinase 1 is essential for normal B cell development and lymphomagenesisNature communicationsNovember 22, 2017Schuler F, Weiss JG, Lindner SE, Lohmüller M, Herzog S, Spiegl SF, Menke P, Geley S, Labi V, Villunger A.(Sigma-Aldrich) and analyzed by immunoblotting. For detection of proteins by chemoluminescence (Advansta, K-12049-D50) a mouse anti-CHK1 (CS 2360, 2G1D5) 1:750, mouse anti-CHK1Ser345 (CS 2348) 1:500, mouse anti-MYC cMYC

(18) MYC selects against reduced BCL2A1/A1 protein expression during B cell lymphomagenesisOncogeneApril 1, 2017Sochalska M, Schuler F, Weiss JG, Prchal-Murphy M, Sexl V, Villunger A.immunoblotting as described.16 For detection of proteins by chemoluminescence (Advansta, Menlo Park, CA, USA, K-12049-D50) a rat anti-mA1 mAb, clone 6D6-1-1,33 rat anti-Bim, clone 3C5 (Enzo, Lausen, Switzerland), rabbit anti-Mcl1

(19) Conditional knockdown of BCL2A1 reveals rate-limiting roles in BCR-dependent B-cell survivalCell death and differentiationApril 1, 2016Sochalska M, Ottina E, Tuzlak S, Herzog S, Herold M, Villunger A.immunoblotting as described.28 For detection of proteins by chemoluminescence (Advansta, Menlo Park, CA, USA; K-12049-D50), a rat anti-mA1 mAb, clone 6D6-1-1,52 rat anti-Bim, clone 3C5 (Enzo, Lausen, Switzerland), rabbit anti-BclxL

(20) The NOXA–MCL1–BIM axis defines lifespan on extended mitotic arrestNature CommunicationsNovember 1, 2015Manuel D. Haschka, Claudia Soratroi, Susanne Kirschnek, Georg Häcker, Richard Hilbe, Stephan Geley, Andreas Villunger, Luca L. Favathroughout the whole procedure. Immunoblots were developed using Western Bright ECL Spray (Advansta K-12049-D50) and either Agfa Curix (34YAX) or GE Healthcare high performance chemoluminescence (28906837) films. Author

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