Phusion High-Fidelity PCR Master Mix with GC Buffer from New England Biolabs

Description

Phusion High-Fidelity PCR Master Mix with GC Buffer - 500 rxns (50 ul vol)

The unique Phusion® High-Fidelity DNA Polymerase offers robust performance and can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is the most accurate thermostable polymerase available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.

Phusion High-Fidelity PCR Master Mix is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl₂. All that is required is the addition of template, primers and water.

Product Specs
ItemPhusion High-Fidelity PCR Master Mix with GC Buffer
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0532L
Quantity500 reactions
ApplicationsDNA Analysis, PCR
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeMaster Mix
Temperature-20 C

Specifications/Features

Information:
Source: An E. coli strain that carries the Phusion DNA Polymerase gene.

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Transcriptional programming in a Bacteroides consortiumNature CommunicationsDecember 1, 2022Brian D. Huang, Thomas M. Groseclose, Corey J. Wilsonassemblies. Q5 polymerase (NEB M0491L) was used for PCR involved in cloning while Phusion polymerase (NEB M0532L) was used for colony PCR. T4 DNA ligase (NEB M0202L) and BsmBI-v2 (R0739L) were used for Golden Gate

(2) A Unique Histone 3 Lysine 14 Chromatin Signature Underlies Tissue-Specific Gene RegulationSSRN Electronic JournalApril 4, 2022Isabel Regadas, Olle Dahlberg, Roshan Vaid, Oanh Ho, Sergey Belikov, Gunjan Dixit, Sebastian Deindl, Jiayu Wen, Mattias Mannervikbeads Beckman Coulter Cat# A63881 Phusion High-Fidelity PCR Master Mix with GC Buffer NEB Cat# M0532L PCR Primer Cocktail Illumina Cat# 15026786 5x HOT FIREPol EvaGreen qPCR Mix Plus Solis Biodyne Cat#

(3) Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungiNew PhytologistApril 1, 2022Sam Keyes et al.high‐fidelity polymerase (M0530L; New England Biolabs, Hitchin, UK) and a PCR master mix with GC buffer (M0532L; New England Biolabs). Following PCR, electrophoresis gels were used to determine whether amplification

(4) PEAC: An Ultrasensitive and Cost-Effective MRD Detection System in Non-small Cell Lung Cancer Using Plasma SpecimenFrontiers in MedicineMarch 3, 2022Jianping Xu, Yue Pu, Rui Lin, Shanshan Xiao, Yingxue Fu, Tao Wangreverse primers (Supplementary Table 3) and Phusion® High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532L). The PCR conditions were 98°C: 3 min, (98°C: 10 s, 60°C: 30 s) × 30), 72°C: 3 min, and stored at 4°C.

(5) High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR ApproachBIO-PROTOCOLJanuary 5, 2020Franziska M Heydenreich, Tamara Miljuš, Dalibor Milić, Dmitry B Veprintsev12931S) 24. Phusion High-Fidelity PCR Master Mix with GC Buffer (New England Biolabs, catalog number: M0532L) or some other appropriate DNA polymerase master mix (see Procedure B. PCR) 25. PEG-8000 (Sigma-Aldrich,

(6) A genome-wide search for epigenetically [corrected] regulated genes in zebra finch using MethylCap-seq and RNA-seqScientific ReportsFebruary 19, 2016Sandra Steyaert et al.21 cycles of PCR amplification using Phusion High-Fidelity PCR Master Mix (New England BioLabs (NEB) M0532L) with primers of the Multiplexing Sample Preparation Oligonucleotide Kit (Illumina). PCR products were

Be the First to Write a Review!