GoTaq® Long PCR Master Mix contains the high-performance GoTaq® Hot Start Polymerase in a specially formulated mixture with a proprietary thermal stable proofreading polymerase. This optimized enzyme mixture allows efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA. The presence of a proofreading enzyme to repair DNA mismatches in the presence of a highly processive polymerase allows the polymerase to continue to elongate the DNA much further, resulting in longer DNA amplification.
The optimized formulation of the GoTaq® Long PCR Master Mix components have been engineered to enable simple reaction setup and provide consistently efficient, accurate and robust performance of long DNA amplicons.
The proven robust amplification of GoTaq® Polymerase is now available for long-range PCR (up to 30kb gDNA).
* Easy: Hot-start master mix for convenient handling and simple setup.
* Enhanced: Yield, sensitivity and specificity with optimized components.
* Accurate: Blend of thermostable DNA polymerases with enhanced processivity and proofreading.
* Confidence: Control primer pair and human gDNA template to control reactions and test template quality.
* Efficient: Perfect for cloning genes, mutational analysis and DNA sequencing.