PhusionHigh-Fidelity PCR Kit from New England Biolabs

Product Specs
ItemPhusionHigh-Fidelity PCR Kit
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberE0553L
Quantity200 reactions
ApplicationsDNA Analysis, PCR
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) GEARBOCS: An Adeno Associated Virus Tool for In Vivo Gene Editing in AstrocytesbioRxiv : the preprint server for biologyJanuary 19, 2023Bindu DS, Tan CX, Savage JT, Eroglu C.638948 Fast SYBR™ Green Master Mix,Applied Biosystems™,Cat#4385612 Phusion® High-Fidelity PCR Kit,NEB,Cat# E0553L ,, Experimental models: cell lines,Experimental models: cell lines, ,, HEK293T,ATCC,CRL-11268 ,, Experimental models

(2) Time-spatial analysis of T cell receptor repertoire in esophageal squamous cell carcinoma patients treated with combined radiotherapy and PD-1 blockadeOncoImmunologyDecember 31, 2022Cihui Yan et al.phusion master mix prepared by using a Phusion® High-Fidelity PCR Kit (New England Biolabs, USA, catalog: E0553L), and nuclease-free water to reach a total volume of 50 μL. The reactions were then transferred to a

(3) Multiple epigenetic factors co-localize with HMGN proteins in A-compartment chromatinEpigenetics & chromatinJune 27, 2022He B, Zhu I, Postnikov Y, Furusawa T, Jenkins L, Nanduri R, Bustin M, Landsman D.1 × NEB buffer 2. The HiC libraries were amplified by using the NEB Phusion High-Fidelity PCR kit (E0553L), using the following PCR conditions: 30 s at 98 °C; seven cycles of 10 s at 98 C, 30 s at 65 °C, 30

(4) An integrated model for Gpr124 function in Wnt7a/b signaling among vertebratesCell reportsMay 31, 2022America M, Bostaille N, Eubelen M, Martin M, Stainier DYR, Vanhollebeke B.,Thermo Fisher Scientific,Cat# 18080–051 In-Fusion HD Cloning Kit,Takara,Cat# 639650 Phusion High-Fidelity PCR Kit,NEB,Cat# E0553L ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, HEK293T,ATCC,CRL-3216;

(5) UNC5C Receptor Proteolytic Cleavage by Active AEP Promotes Dopaminergic Neuronal Degeneration in Parkinson's DiseaseAdvanced science (Weinheim, Baden-Wurttemberg, Germany)March 1, 2022Chen G, Ahn EH, Kang SS, Xia Y, Liu X, Zhang Z, Ye K.(Covance), Recombinant Mouse Netrin‐1 Protein (R&D systems, 1109‐N1/CF), Phusion High‐Fidelity PCR Kit (NEB, E0553L), In Situ Cell Death Detection Kit, TMR red (Roche, 12156792910), QuikChange Lightning Site‐Directed Mutagenesis Kit

(6) Fluorescence activation mechanism and imaging of drug permeation with new sensors for smoking-cessation ligandseLifeJanuary 4, 2022Aaron L Nichols et al.177756,Obtainable through Addgene Commercial assay or kit,Phusion High-Fidelity PCR Kit,New England Biolabs,E0553L, Commercial assay or kit,Q5 Site-Directed Mutagenesis Kit,New England Biolabs,E0554S, Commercial assay

(7) Netrin-1 receptor UNC5C cleavage by active δ-secretase enhances neurodegeneration, promoting Alzheimer's disease pathologiesScience advancesApril 1, 2021Chen G, Kang SS, Wang Z, Ahn EH, Xia Y, Liu X, Sandoval IM, Manfredsson FP, Zhang Z, Ye K.Lab), Recombinant Mouse Netrin-1 Protein (R&D Systems, 1109-N1/CF), Phusion High-Fidelity PCR Kit (NEB, E0553L), Recombinant Human Caspase-3 Protein (Novus, H00000836-P02), AENK (Millipore, 5.33796.0001), Pan Caspase Inhibitor Z-VAD-FMK

(8) Metagenomic 16S rDNA amplicon data of microbial diversity of guts in Vietnamese humans with type 2 diabetes and nondiabetic adultsData in BriefFebruary 1, 2021Hung The Hoang, Duc Hoang Le, Thi Thanh Huyen Le, Thi Tuyet Nhung Nguyen, Ha Hoang Chu, Nam Trung Nguyenparallel. All PCR reactions were performed with Phusion® High-Fidelity PCR Master Mix (catalogue No. E0553L, New England Biolabs, UK). Sequencing libraries were generated by using TruSeq® DNA PCR-Free Library

(9) Autoregulation of yeast ribosomal proteins discovered by efficient search for feedback regulationCommunications BiologyDecember 1, 2020Basab Roy, David Granas, Fredrick Bragg, Jonathan A. Y. Cher, Michael A. White, Gary D. Stormounder a T7 promoter. DNAs were amplified by PCR following the Phusion High-Fidelity PCR (NEB, catalog # E0553L) protocol. One microgram of the PCR product (linearized duplex DNA with 5′ T7 promoter and UTR or the

(10) Concentration-dependent splicing is enabled by Rbfox motifs of intermediate affinityNature Structural & Molecular BiologyOctober 1, 2020Bridget E. Begg, Marvin Jens, Peter Y. Wang, Christine M. Minor, Christopher B. Burgeperformed as previously described26,28,38. Briefly: Library was amplified with Phusion Polymerase (NEB, #E0553L, primers below, Integrated DNA Technologies (IDT)), in vitro-transcribed, treated with Turbo DNase (Thermofisher,

(11) Community Structure of Arbuscular Mycorrhizal Fungi in Soils of Switchgrass Harvested for BioenergyApplied and Environmental MicrobiologySeptember 17, 2020Alden C. Dirks, Randall D. Jacksonhttps://www.pacb.com/wp-content/uploads/Procedure-Checklist-Preparing-SMRTbell-Libraries-using-PacBio-Barcoded-Universal-Primers-for-Multiplexing-Amplicons.pdf. PCRs were prepared using the Phusion high-fidelity PCR kit from New England Biolabs (catalog no. E0553L) in 25-μl reaction mixtures, as follows: 1 μl of template DNA at a concentration of 0.1 to 40 ng · μl−1,

(12) A comparison of humans and baboons suggests germline mutation rates do not track cell divisionsPLOS BiologyAugust 17, 2020Felix L. Wu, Alva I. Strand, Laura A. Cox, Carole Ober, Jeffrey D. Wall, Priya Moorjani, Molly Przeworskispecificity using NCBI PrimerBlast [89]. Using the high-fidelity Phusion polymerase (New England BioLabs E0553L), we attempted to PCR-amplify the 87 target sites in all 3 members of the H14 trio (H14, H11, H12).

(13) Design and Construction of a Focused DNA-Encoded Library for Multivalent Chromatin Reader ProteinsMoleculesFebruary 22, 2020Justin M. Rectenwald et al.ThermoFisher Scientific (catalog number 65001). A Phusion High-Fidelity Polymerase PCR kit (catalog number E0553L), T4 DNA Ligase (catalog number M0202M), and DNA Polymerase I, Large (Klenow) Fragment (catalog M0210M)

(14) A Receptor of the Immunoglobulin Superfamily Regulates Adaptive ThermogenesisCell ReportsJuly 16, 2019Carmen Hurtado Hurtado del Pozo et al."DMEM, high glucose, HEPES, no phenol red",Life Technologies,21063-029 Phusion® High-Fidelity PCR Kit,"New England Biolabs, Inc",E0553L Dual-Glo® Luciferase Assay System,Promega,E2940 "Glo Lysis Buffer, 1X",Promega,E2661 QIAzol Lysis

(15) CRISPR–Cas9 genome engineering of primary CD4+ T cells for the interrogation of HIV–host factor interactionsNature ProtocolsJuly 9, 2019Judd F. Hultquist, Joseph Hiatt, Kathrin Schumann, Michael J. McGregor, Theodore L. Roth, Paige Haas, Jennifer A. Doudna, Alexander Marson, Nevan J. KrogandNTPs (NEB, cat. no. N0447L) Phusion High-Fidelity (HF) PCR Kit with 5× HF buffer (NEB, cat. no. E0553L) Oligonucleotides for TIDE analysis (IDT, target-specific sequences) Formaldehyde (Sigma, cat. no.

(16) Engineered Orthogonal Quorum Sensing Systems for Synthetic Gene Regulation in Escherichia coliFrontiers in Bioengineering and BiotechnologyApril 17, 2019Stefan J. Tekel, Christina L. Smith, Brianna Lopez, Amber Mani, Christopher Connot, Xylaan Livingstone, Karmella A. Haynes5′-GGATTTCTGCAGCGGCCGCTACTAGTA with the following conditions: in a 50 μL reaction: 10 μL 5x HF buffer (New England Biolabs, NEB #E0553L), 2 μL 10 mM dNTP mix (NEB #N0447L), 1 μL 100 μM forward and reverse primer, 25 ng g block template,

(17) Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterningeLifeNovember 15, 2017Jose L Pelliccia, Granton A Jindal, Rebecca D Burdinewater. PCR was performed using 5 μl of the diluted DNA extract. Phusion High-Fidelity PCR Kit (NEB, E0553L) was used with forward primer 5’-CAGATCCCAAAATCTTCATGC-3’ and reverse primer 5’-AAGTGTGGTCCGTAAGATCCTC-3’

(18) Osteoblastic Lrp4 promotes osteoclastogenesis by regulating ATP release and adenosine-A_2AR signalingThe Journal of cell biologyMarch 6, 2017Xiong L, Jung JU, Guo HH, Pan JX, Sun XD, Mei L, Xiong WC.CCA) point mutations were made from the Flag-Lrp4 construct using the Phusion High-Fidelity PCR kit (E0553L; New England Biolabs, Inc.) and Restriction Endonucleases DpnI (R0176L; New England Biolabs, Inc.).

(19) Production of Guide RNAs in vitro and in vivo for CRISPR Using Ribozymes and RNA Polymerase II PromotersBIO-PROTOCOLFebruary 20, 2017Tao Zhang, Yangbin Gao, Rongchen Wang, Yunde Zhao(New England Biolabs, catalog number: M0208L) Phusion High-Fidelity PCR Kit (New England Biolabs, catalog number: E0553L) LB medium Appropriate antibiotics QIAGEN Plasmid Mini Kit MfeI (New England Biolabs, catalog

(20) A rationally designed nanoparticle for RNA interference therapy in B-lineage lymphoid malignanciesEBioMedicineJanuary 20, 2015Fatih M. Uckun, Sanjive Qazi, Hong Ma, Lichen Yin, Jianjun Chenggenerated by PCR amplification using the Phusion High Fidelity PCR Kit (New England Biolabs, catalog no. E0553L) with the following primer sets: full-length Fwd/Rev 5′-CTTGGTGCTAGCATGCATCTCCTCGGC-3′/5′CCGGTCTCGAGGATGTTTGAGGATCACATAGTC-3′,

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