Nuclei Lysis Solution from Promega

Product Specs
ItemNuclei Lysis Solution
CompanyPromega
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberA7943
Quantity1 liter
Nucleic Acid TypeGenomic DNA

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) ANT-dependent MPTP underlies necrotic myofiber death in muscular dystrophyScience advancesAugust 25, 2023Bround MJ, Havens JR, York AJ, Sargent MA, Karch J, Molkentin JD.(Thermo Scientific). Briefly, tissue was digested overnight at 55°C in Nuclei Lysis Solution (Promega; PR-A7943) supplemented with 0.1 M EDTA (VWR), Proteinase K (1 mg/ml; Thomas Scientific; C755F45), and RNAse A

(2) RNAi suppression of xylan synthase genes in wheat starchy endospermPLOS ONEAugust 19, 2021Mark D. Wilkinson, Ondrej Kosik, Kirstie Halsey, Hannah Walpole, Jessica Evans, Abigail J. Wood, Jane L. Ward, Rowan A. C. Mitchell, Alison Lovegrove, Peter R. Shewrywas extracted from leaf material (Promega Wizard genomic DNA purification kit; Nuclei Lysis Solution A7943; Protein Preciptation Solution A7953; Promega UK Ltd- 2 Benham Road, Southampton Science Park, Chilworth

(3) Role for Cell-Surface Collagen of Streptococcus pyogenes in InfectionsACS Infectious DiseasesJuly 10, 2020Aubrey J. Ellison, Felix Dempwolff, Daniel B. Kearns, Ronald T. Rainesgently. The solution was then incubated for 30 min at 37 °C, and 600 μL of nuclei lysis solution (Promega A7943) was added. The resulting mixture was incubated for 2–3 min at room temperature, 200 μL of protein precipitation

(4) High quality reference genomes for toxigenic and non-toxigenic Vibrio cholerae serogroup O139Scientific ReportsDecember 1, 2019Matthew J. Dorman, Daryl Domman, Muhammad Ikhtear Uddin, Salma Sharmin, Mokibul Hassan Afrad, Yasmin Ara Begum, Firdausi Qadri, Nicholas R. Thomson25% w/v sucrose in TE buffer (10 mM Tris pH 8.0, 1 mM EDTA pH 8.0). Nuclei Lysis Solution (Promega, #A7941, 6.0 ml) was added and samples were lysed by incubation at 80 °C for five minutes. Samples were mixed

(5) Brush border protocadherin CDHR2 promotes the elongation and maximized packing of microvilli in vivoMolecular biology of the cellJanuary 1, 2019Pinette JA, Mao S, Millis BA, Krystofiak ES, Faust JJ, Tyska MJ.genotyping, villus-enriched epithelial fractions were incubated in Nuclei Lysis Solution (Promega #A7941) with EDTA and Proteinase K at 55°C for 1 h. Protein Precipitation Solution (Promega #A795A) was then

(6) Absence of MGST1 mRNA and protein expression in human neuroblastoma cell lines and primary tissueFree Radical Biology and MedicineApril 1, 2014Michael J. Kelner, Mitchell B. Diccianni, Alice L. Yu, Mary R. Rutherford, Leita A. Estes, Ralf Morgensternextracted from cells by the Promega Wizard Genomic DNA purification method (Promega Lysis Solution #A7943, Protein Precipitation Solution #A7953, and RNase Solution #A7973) and DNA content quantified by spectrophotometry.

(7) Krüppel like factor 4 promoter undergoes active demethylation during monocyte/macrophage differentiationPloS oneJanuary 1, 2014Karpurapu M, Ranjan R, Deng J, Chung S, Lee YG, Xiao L, Nirujogi TS, Jacobson JR, Park GY, Christman JW.(sc-8035). Genomic DNA was isolated using Promega genomic DNA isolation reagents (Nuclei lysis solution #A7943; Protein precipitation solution #A7953), Bisulfite conversion of methylated genomic DNA was carried

(8) Single-molecule and population probing of chromatin structure using DNA methyltransferasesMethodsMarch 1, 2007Jessica A KILGORE, Scott A HOOSE, Tanya L GUSTAFSON, Weston PORTER, Michael P KLADDEManuscript 1. Promega Wizard genomic DNA purification kit (cat. #A1120). 2. Promega nuclei lysis solution (cat. #A7941). 3. Promega RNase A solution, 4 mg/ml (cat. #A7973). 4. Promega protein precipitation solution

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