PureGenome™ library preparation is a simple, two-step process followed by amplification using EMD Millipore's ultra-high fidelity KOD Hot Start DNA Polymerase Mastermix. This unique polymerase amplifies DNA with high processivity in highly TA- or GC-rich regions. The combined efficiency of library construction and accuracy of amplification enables maximum library yields from lower input DNA with minimal bias.
Sequencing data from libraries prepared using the PureGenome™ NGS library preparation reagents are of the highest quality with respect to Phred scoring (Q20>85%), minimal bias in GC coverage and higher ratios of matched:unmatched reads. Ultimately, better sequencing data translates into more meaningful biological results, especially in applications for which sensitivity and specificity are crucial, such as gene expression analysis or single-nucleotide polymorphism studies. The PureGenome™ NGS library preparation reagents are validated for Illumina® platform-compatible NGS libraries; however, end users have the flexibility to optimize for other platforms.
Supporting kits in the PureGenome™ workflow include the PureGenome™ Tissue DNA Extraction Kit, specifically validated for NGS library preparation from solid tissue, biological fluids and cell cultures, and the PureGenome™ On-Spot Tissue DNA Kit, which is used for extraction of DNA from targeted areas on tissue sections and enables analysis from minute samples. The PureGenome™ NGS Library Validator Kit enables highly accurate and cost-effective qPCR quantification of Illumina® platform-compatible NGS libraries.
More information, visit www.millipore.com/ngs