Mouse Primary iPS Cells-Nneo from Stemgent

Mouse Primary iPS Cells-Nneo from Stemgent

Description

Stemgent Mouse Primary iPS Cells-NNeo harbor the reverse tetracycline transactivator (rtTA) targeted to the ROSA26locus (ROSA26-M2rtTA), Oct4 cDNA under the control of tetracycline operator targeted to the type I collagen locus, a neomycin (neo) resistance gene in the endogenous Nanog locus, and the DOX-inducible transcription factors [Klf4 (3 proviral integrations), Sox2 (2 proviral integrations) and c-Myc (1 proviral integrations)] inserted during lentivirus transduction. Injection of these cells into blastocysts results in the generation of chimeric mice from which a variety of cell types (neural progenitor, adrenal gland, keratinocyte, muscle, and mouse embryonic fibroblast) can be isolated for secondary reprogramming studies1. The simple addition of DOX to the growth medium used to propagate the isolated cell types from the chimeric mouse results in the upregulation in expression for the aforementioned 3 transcription factors implicated in the reprogramming process.

Specifications

Stemgent
Stemgent
10575 Roselle Street
San Diego, California 92121
United States
Phone: 1.877.2BUYSTEM
1.617.245.0098
1.617.245.0097