Pre-miR™ miRNA Precursor, 5 nmol from Ambion

Description

  • Detailed Specifications
  • ItemPre-miR™ miRNA Precursor, 5 nmol
  • CompanyAmbion
  • PricePricing InfoGet QuoteGet a Quote for this Product
  • Catalog NumberAM17100
  • Quantity5 nmol
  • ReactivityInquire
  • TagInquire
  • TypemiRNA Inhibitor
  • TargetmiRNA
  • SpeciesInquire
  • Target/Molecule DescriptorPre-miR™
  • Original Item NamePre-miR™ miRNA Precursor Molecule

Product Description

A synthetic miRNA mimic for miRNA functional analysis and target site validation. 5 nmol is supplied.



• Ambion® Pre-designed molecules for miRNAs listed in miRBase—custom design available

• Optimized design results in correct strand utilization by RISC

• Purified and ready to transfect



Ambion® Pre-miR™ miRNA Precursor Molecules are small, chemically modified, double-stranded RNA molecules designed to mimic endogenous mature miRNAs. This ready-to-use miRNA mimic can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. They enable detailed study of miRNA biological effects via miRNA gain-of-function experiments, such as:



• Screening for miRNAs that regulate the expression of a gene

• miRNA target site identification and validation

• Screening for miRNAs that affect a cellular process



To select your miRNA sequence and order, please visit the GeneAssist™ miRNA Workflow Builder.



Accessory Products:

miRNA-related cellular functions can be further investigated using Anti-miR™ miRNA Inhibitors (SKU #AM17000 and #AM17001), which enable loss-of-function experiments through reduction of miRNA activity. To select your miRNA sequence and order, please visit the GeneAssist™ miRNA Workflow Builder. The Pre-miR™ miRNA Starter Kit (SKU #AM1540) can be used to optimize transfection of Pre-miR™ miRNA Precursors.

References

Inquire
Ambion
Ambion
2130 Woodward
Austin, Texas 78744
United States
Phone: 512-651-0200
512-651-0201
Company Profile
Website: http://www.ambion.com

  • Reagents For Up And Down-Regulation Of MiRNA Activity In Mammalian Cells

    More than 200 miRNAs have been cloned and sequenced from human and mouse samples. Each miRNA apparently regulates multiple genes, and miRNAs are thought to regulate as many as 10% of human genes [1]. Several reports predict mRNA target sites for miRNAs [2,3]. A handful of these predicted sites have been verified by introducing miRNAs into cells and monitoring the expression of the putative target gene, but thousands of predicted sites remain to be verified. To facilitate these studies, Ambion has developed a series of precursor miRNAs (Pre-miR™ miRNA Precursors) and a series of miRNA inhibitors (Anti-miR™ miRNA Inhibitors). Read More

  • Precursor MiRNAs For Successful MiRNA Functional Studies

    MicroRNAs (miRNAs) are small, regulatory RNAs that are expressed in animals and plants and affect the translation or stability of target mRNAs. The 17-24 nt, single-stranded (ss) miRNAs are derived from longer, primary transcripts termed "pri-miRNAs" [1]. The pri-miRNAs, which can be more than 1000 nt in length, contain an RNA hairpin in which one of the two strands includes the mature miRNA [1]. The hairpin, which typically comprises 60-120 nt, is cleaved from the pri-miRNA in the nucleus by the double-strand-specific ribonuclease, Drosha [1]. The resulting precursor miRNA, or "pre-miRNA," is transported to the cytoplasm via a process that involves Exportin-5 [2-4]. The pre-miRNA is further cleaved by Dicer [5] to generate a short, partially double-stranded (ds) RNA in which one strand is the mature miRNA. The mature miRNA is taken up by a protein complex that is similar, if not identical, to the RNA Induced Silencing Complex (RISC) that supports RNA interference (RNAi) [6], and miRNA-bound complex functions to regulate translation. Read More

  • Cancer-Related MiRNAs Uncovered By The mirVana™ MiRNA Microarray Platform

    Ambion has developed a complete line of research tools for the analysis of microRNAs (miRNAs). Recently, we introduced the mirVana™ miRNA microarray platform (consisting of the mirVana miRNA Labeling Kit and the mirVana miRNA Probe Set) and several miRNA tools for interrogating miRNA function and identifying miRNA target sites (Pre-miR™ miRNA Precursor Molecules, Anti-miR™ miRNA Inhibitors, and the pMIR-REPORT™ miRNA Expression Reporter Vector). In this article, these new tools are used to identify and characterize potential effectors of cancer progression. Read More