Blotto, non-fat dry milk from Santa Cruz Biotechnology, Inc.

Product Specs
ItemBlotto, non-fat dry milk
CompanySanta Cruz Biotechnology, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Numbersc-2324
Quantity250 g
TypeBlocking Reagents

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Mass isolation of staged Drosophila pupal intestines for analysis of protein ubiquitylationSTAR ProtocolsDecember 1, 2023Ruoxi Wang, Panagiotis D. Velentzas, Eric H. BaehreckeBCA protein assay kits,Thermo Scientific,23225 "Blotto, non-fat dry milk",Santa Cruz Biotechnology,sc-2324 10× Tris/glycine/SDS,Bio-Rad,1610732 Triton X-100,Sigma-Aldrich,T8787 NaCl,Fisher Scientific,BP358-212 KCl,Fisher

(2) USP24-dependent stabilization of Runx2 recruits a p300/NCOA3 complex to transactivate ADAMTS genes and promote degeneration of intervertebral disc in chronic inflammation miceBiology DirectJuly 6, 2023Xingguo Li, Jun Zhang, Bing Wang, Chao Chen, Enyu Zhang, Zhengpin Lv, Qicong He, Yaoquan Hu, Xuenan Wang, Fan ZhangScientific; #88,518). After incubation with 5% non-fat milk (Santa Cruz Biotechnology; Shanghai, China; #sc2324), the PVDF membranes were probed with primary antibodies (Table S4) and secondary antibodies (Table

(3) Distinct hyperactive RAS/MAPK alleles converge on common GABAergic interneuron core programsDevelopmentMay 15, 2023Sara J. Knowles, April M. Stafford, Tariq Zaman, Kartik Angara, Michael R. Williams, Jason M. Newbern, Daniel Vogtsaline with 0.1% Tween 20 (TBST) and blocked for 1 h in TBST containing 5% non-fat dry milk (blotto, sc-2324 Santa Cruz Biotechnology). Membranes were incubated with primary antibodies overnight at 4°C, washed

(4) Decidualization of human endometrial stromal cells requires steroid receptor coactivator-3Frontiers in Reproductive HealthNovember 24, 2022Vineet K. Maurya, Maria M. Szwarc, David M. Lonard, William E. Gibbons, San-Pin Wu, Bert W. O’Malley, Francesco J. DeMayo, John P. Lydonmembranes were blocked for 1 h with 5% non-fat dry milk [Santa Cruz Biotechnology Inc., Dallas, TX; (# SC-2324)] in Tris-buffered saline with Tween 20 (TBS-T) and incubated overnight at 4°C with the following primary

(5) A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasmaNature Biomedical EngineeringAugust 8, 2022Devora Najjar, Joshua Rainbow, Sanjay Sharma Timilsina, Pawan Jolly, Helena de Puig, Mohamed Yafia, Nolan Durr, Hani Sallum, Galit Alter, Jonathan Z. Li, et al.E9508) dissolved in 10 mM PBS at pH 7.4 for 30 min and blocked with 5% Blotto (Santa Cruz Biotechnology, sc-2324) in 10 mM PBS containing 0.05% Tween 20 (Sigma Aldrich, P9416). The fabricated sensor was then used

(6) Nutrient sensitive protein O-GlcNAcylation modulates the transcriptome through epigenetic mechanisms during embryonic neurogenesisLife Science AllianceAugust 1, 2022Shama Parween, Thilina T Alawathugoda, Ashok D Prabakaran, S Thameem Dheen, Randall H Morse, Bright Starling Emerald, Suraiya A Ansarifor 1 h at 4°C. After transfer, the membrane was blocked using 5% Blotto, non-fat dry milk (Cat. no. sc-2324; Santa Cruz Biotechnology) or 5% BSA (Cat. no. A7030; Sigma-Aldrich) for 1 h at RT. The membrane was

(7) A Synthetic Small RNA Homologous to the D-Loop Transcript of mtDNA Enhances Mitochondrial BioenergeticsFrontiers in PhysiologyApril 6, 2022Theodore L. Mathuram et al.0.1% of Tween (TBST) supplemented with 5% of non-fat dry milk (Blotto; Santa Cruz Biotechnology, cat. #sc-2324) for 1 h at room temperature, washed with TBST twice, and incubated with primary antibodies in either

(8) MACC1 regulates the AKT/STAT3 signaling pathway to induce migration, invasion, cancer stemness, and suppress apoptosis in cervical cancer cellsBioengineeredJanuary 1, 2022Jie Mei, ChengYa Zhu, LiuLiu Pan, Mian Lifluoride membranes (PVDF, Milipore, USA). After incubating in 5% nonfat milk (Santa Cruz Biotechnology, sc-2324, USA) for 2 h, the membranes were incubated with primary antibodies at 4°C for 10 h. Next, the PVDF

(9) YAP facilitates melanoma migration through regulation of actin-related protein 2/3 complex subunit 5 (ARPC5)Pigment cell & melanoma researchJanuary 1, 2022Lui JW, Moore SPG, Huang L, Ogomori K, Li Y, Lang D.(Proteintech – 22025-1-AP), 1:10000 for GAPDH (Cell Signaling – D16H11)) in nonfat 5% milk (Santa Cruz – sc-2324). The membranes were washed with 1X TBS-T four times for 15 minutes and incubated with 1:4000 anti-rabbit IgG HRP-linked

(10) Striatal dopaminergic neurons as a potential target for GDNF based ischemic stroke therapyTURKISH JOURNAL OF MEDICAL SCIENCESNovember 11, 2021Mustafa Çağlar Beker, Merve Beker, Ahmet Burak Çağlayan, Busenur Bolat, Ülkan Kılıç, Gamze Torun Köse, Ertuğrul KılıçNF-κB (ab16502, Abcam) and tyrosine hydroxylase (AB152, Millipore) were diluted in %5 non-fat dry milk (sc-2324, Santa Cruz Biotechnology) with TBS-T and incubated overnight on membranes. Next day after several washing

(11) Spatial Stem Cell Fate Engineering via Facile Morphogen LocalizationAdvanced healthcare materialsNovember 1, 2021Jin G, Floy ME, Simmons AD, Arthur MM, Palecek SP.times, and incubated in diluted primary antibodies in nonfat dry milk (5%) (Santa Cruz Biotechnology, sc-2324)/Triton X-100 (0.4%) (Fisher Scientific, BP151-500) PBS overnight at 4°C. Then, the cells were washed

(12) The role of circadian rhythm in the regulation of cellular protein profiles in the brainTURKISH JOURNAL OF MEDICAL SCIENCESOctober 21, 2021Mustafa Çağlar Caglar Beker, Ertuğrul KILIÇBio-Rad, Hercules, CA, USA). Thereafter, the membranes were blocked in 5% nonfat dry milk (Blotto; sc-2324, Santa Cruz Biotechnology, Dallas, TX, USA) in 50 mMol Tris-buffered saline (TBS) containing 0.1% Tween (TBS-T;

(13) TBK1 Inhibitor Exerts Antiproliferative Effect on Glioblastoma Multiforme CellsOncology Research Featuring Preclinical and Clinical Cancer TherapeuticsSeptember 7, 2021Sarah A. Scuderi, Marika Lanza, Giovanna Casili, Francesca Esposito, Cristina Colarossi, Dario Giuffrida, Paterniti Irene, Salvatore Cuzzocrea, Emanuela Esposito, Michela CampoloAbcam), and anti-IFN-γ (1:500; sc-390800; Santa Cruz Biotechnology) in 1 × PBS, 5% w/v nonfat dried milk (sc-2324; Santa Cruz Biotechnology), 0.1% Tween-20 (P9416; Sigma-Aldrich) at 4°C overnight. The day after, the

(14) Who''s in, who''s out? Re-evaluation of lipid raft residentsJournal of neurochemistryAugust 1, 2021Mlinac-Jerkovic K, Ilic K, Zjalić M, Mandić D, Debeljak Ž, Balog M, Damjanović V, Maček Hrvat N, Habek N, Kalanj-Bognar S, et al.Macherey‐Nagel. Non‐fat dry milk used for blocking in western blotting was from Santa Cruz Biotechnology (cat.no. sc‐2324). Primary antibody for transferrin receptor (monoclonal mouse anti‐TfR, RRID:AB_2533029) and horseradish

(15) Alzheimer’s protection effect of A673T mutation may be driven by lower Aβ oligomer binding affinityJournal of NeurochemistryMay 1, 2021Colleen S. Limegrover, Harry LeVine, Nicholas J. Izzo, Raymond Yurko, Kelsie Mozzoni, Courtney Rehak, Kelsey Sadlek, Hank Safferstein, Susan M. Catalanomembrane was boiled in PBS for 5 min and blocked with 5% Blotto (Santa Cruz Biotechnology; catalog SC‐2324; year of purchase 2014) overnight at 4°C. The membrane was probed for 1 hr at 20°C with 6E10 monoclonal

(16) Eltrombopag Improves Erythroid Differentiation in a Human Induced Pluripotent Stem Cell Model of Diamond Blackfan AnemiaCellsMarch 26, 2021Husam Qanash, Yongqin Li, Richard H. Smith, Kaari Linask, Sara Young-Baird, Waleed Hakami, Keyvan Keyvanfar, John S. Choy, Jizhong Zou, Andre Larochellemembranes (PB5210, Thermo Fisher Scientific). Membranes were blocked with Blotto, non-fat dry milk (sc-2324, Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 1 h at 4 °C, followed by incubation with 1:1000

(17) EpCAM-Targeted 3WJ RNA Nanoparticle Harboring Delta-5-Desaturase siRNA Inhibited Lung Tumor Formation via DGLA PeroxidationMolecular Therapy - Nucleic AcidsDecember 4, 2020Lizhi Pang, Harshit Shah, Hongzhi Wang, Dan Shu, Steven Y. Qian, Venkatachalem Sathishprogram as the instruction manual. After transfer, the membrane was incubated in the 5% non-fat dry milk (sc-2324; Santa Cruz Biotechnology) for 1 h to block the non-specific binding. Then the PVDF membrane was incubated

(18) Generation of Functional Mouse Hippocampal NeuronsBIO-PROTOCOLAugust 5, 2020Francesco Tomassoni-Ardori, Zhenyi Hong, Gianluca Fulgenzi, Lino Tessarollo(Sigma Aldrich, catalog number: P1379) Non-fat dry milk (Santa Cruz Biotechnology, catalog number: sc-2324) Super Signal West Dura Extended Duration Substrate (Thermo Fisher Scientific, catalog number: 34076)

(19) DT2216-a Bcl-xL-specific degrader is highly active against Bcl-xL-dependent T cell lymphomasJournal of hematology & oncologyJuly 16, 2020He Y, Koch R, Budamagunta V, Zhang P, Zhang X, Khan S, Thummuri D, Ortiz YT, Zhang X, Lv D, et al.(TBST, Cat. No. J77500, Affymetrix, Santan Clara, CA, USA) containing 5% non-fat dry milk (Cat. No. sc-2324, Santa Cruz Biotechnology, Dallas, TX, USA) and subsequently probed with primary antibodies at a predetermined

(20) Using proteolysis-targeting chimera technology to reduce navitoclax platelet toxicity and improve its senolytic activityNature communicationsApril 24, 2020He Y, Zhang X, Chang J, Kim HN, Zhang P, Wang Y, Khan S, Liu X, Zhang X, Lv D, et al.(TBST, Cat. No. J77500, Affymetrix, Santan Clara, CA, USA) containing 5% non-fat dry milk (Cat. No. sc-2324, Santa Cruz Biotechnology, Dallas, TX, USA), and subsequently probed with primary antibodies at a predetermined

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Blotto, non-fat dry milk from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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Blotto, non-fat dry milk from Santa Cruz Biotechnology, Inc.

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