Nonfat Dry Milk from Cell Signaling Technology

Product Specs
ItemNonfat Dry Milk
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number9999S
Quantity250 g
Research AreaRelated Products
ApplicationsW

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Variants in the CETP gene affect levels of HDL cholesterol by reducing the amount, and not the specific lipid transfer activity, of secreted CETPPLoS OneDecember 1, 2023Åsa Schawlann Ølnes, Marianne Teigen, Jon K. Laerdahl, Trond P. Leren, Thea Bismo Strøm, Katrine Bjuneweight marker. Non-specific binding sites were blocked using 5% Blotting Grade Blocker Non-Fat Dry Milk (9999S; Cell Signaling Technology, Danvers, MA) for 1 h at 22°C. A horseradish peroxidase-conjugated mouse

(2) Exploiting TLK1 and Cisplatin Synergy for Synthetic Lethality in Androgen-Insensitive Prostate CancerBiomedicinesNovember 7, 2023Siddhant Bhoir, Oluwatobi Ogundepo, Xiuping Yu, Runhua Shi, Arrigo De Benedettimembrane was blocked with 5% non-fat dry milk (Cell Signaling Technology, Danvers, MA, USA; Cat. No. 9999S) in 1X Tris-buffered saline with Tween-20 (TBST) for 1 h at room temperature. Following blocking, the

(3) Critical Velocity, Maximal Lactate Steady State, and Muscle MCT1 and MCT4 after Exhaustive Running in MiceInternational Journal of Molecular SciencesOctober 30, 2023Juan B. Orsi, Lara S. Araujo, Pedro P. M. Scariot, Emanuel E. C. Polisel, Luisa O. Cardoso, Claudio A. Gobatto, Fúlvia B. Manchado-Gobattoof a Li-COR Odyssey Fc imaging system. Blocking was performed in 1% milk (skimmed milk powder, cat# 9999, Cell Signaling Technology®, Danvers, MA, USA) in PBS (10 mL of PBS, 0.1 g of milk-NFDM) for 1 h and

(4) Synergistic roles of CBX4 chromo and SIM domains in regulating senescence of primary human osteoarthritic chondrocytesArthritis Research & TherapyOctober 12, 2023Yu-Hsiu Chen, Xin Zhang, David Attarian, Virginia Byers KrausTrans-blot turbo system (Bio-Rad,#1704274). The membrane was blocked with 5% w/v fat-free milk (CST 9999 s) for 1 h at room temperature. Membranes were washed three times with Tris-buffered saline Tween 20

(5) (‐)‐Epigallocatechin‐3‐gallate induced apoptosis by dissociation of c‐FLIP/Ku70 complex in gastric cancer cellsJournal of Cellular and Molecular MedicineSeptember 1, 2023Mahtab Shahriari Felordi, Mehdi Alikhani, Zahra Farzaneh, Mahmoud Alipour Choshali, Marzieh Ebrahimi, Hamidreza Aboulkheyr Es, Abbas Piryaei, Mustapha Najimi, Massoud Vosough(Sigma‐Aldrich. P2850); BCA assay (Thermo Fisher Scientific); nonfat dry milk (Cell Signaling Technology, #9999S) from; ECL western blotting detection reagent (Thermo Scientific, RPN2232); 0.1% NP‐40 (Sigma‐Aldrich,

(6) Ze-Qi decoction inhibits non-small cell lung cancer growth and metastasis by modulating the PI3K/Akt/p53 signaling pathwayJournal of Traditional and Complementary MedicineSeptember 1, 2023Jingtao Zhang, Zifan Zhuang, Minghao Guo, Kai Wu, Qingfeng Yang, Xin Min, Wenqiang Cui, Fei Xupolyvinylidene difluoride membranes (Millipore, MA, USA). The membranes were blocked with 5% non-fat dry milk (9999S; Cell Signaling Technology, Danvers, MA, USA) for 2 h, then incubated with primary antibodies at 4 °C

(7) Single-dose mucosal replicon-particle vaccine protects against lethal Nipah virus infection up to 3 days after vaccinationSci AdvAugust 4, 2023Stephen R. Welch, Jessica R. Spengler, Sarah C. Genzer, JoAnn D. Coleman-McCray, Jessica R. Harmon, Teresa E. Sorvillo, Florine E. M. Scholte, Sergio E. Rodriguez, T. Justin O'Neal, Jana M. Ritter, et al.washer. Wells were blocked for 1 hour at 37°C with blocking buffer [5% milk (Cell Signaling Technology, 9999S) in wash buffer (TBS-T)]. Serially diluted plasma in blocking buffer was added to the blocked plates

(8) Comprehensive characterization of human brain-derived extracellular vesicles using multiple isolation methods: Implications for diagnostic and therapeutic applicationsJournal of extracellular vesiclesAugust 1, 2023Zhang Z, Yu K, You Y, Jiang P, Wu Z, DeTure MA, Dickson DW, Ikezu S, Peng J, Ikezu T.nitrocellulose membranes (Cat# 1620115, Bio‐Rad). The membranes were blocked with 5% non‐fat milk (Cat# 9999S, Cell Signaling Technology) and incubated with primary antibodies overnight at 4°C. The following primary

(9) The Extract of Scutellaria baicalensis Attenuates the Pattern Recognition Receptor Pathway Activation Induced by Influenza A Virus in MacrophagesVirusesJuly 8, 2023Yang M, Ma L, Su R, Guo R, Zhou N, Liu M, Wu J, Wang Y, Hao Y.(No. ISEQ00010, Millipore, Billrica, MA, USA), then blocked in TBST solution with 5% non-fat milk (No. 9999S, CST, USA) for 1 h. The PVDF membranes were incubated with anti-NP (1:1000, mouse, No. ab128193, Abcam,

(10) Inherited ARPC5 mutations cause an actinopathy impairing cell motility and disrupting cytokine signalingNature communicationsJune 22, 2023Nunes-Santos CJ, Kuehn H, Boast B, Hwang S, Kuhns DB, Stoddard J, Niemela JE, Fink DL, Pittaluga S, Abu-Asab M, et al.(Invitrogen). All membranes were blocked in 5% (wt/vol) of nonfat dry milk (Cell Signaling Technology, Cat. 9999) in 1X Tris-buffered saline with 0.1% Tween 20 (TBS-T, Cell Signaling Technology, Cat. 9997), incubated

(11) Evaluation of therapeutic PD-1 antibodies by an advanced single-molecule imaging system detecting human PD-1 microclustersNature communicationsJune 6, 2023Nishi W, Wakamatsu E, Machiyama H, Matsushima R, Saito K, Yoshida Y, Nishikawa T, Takehara T, Toyota H, Furuhata M, et al.citrate buffer, pH 4.5, for 24 h at 37 °C, blocked with 5% nonfat dried milk (Cell Signaling Technology, 9999S) in PBS for 30 min at 37 °C, removed the cover glass, and left to stand in the assay medium (Hepes-buffered

(12) SPINK2 Protein Expression Is an Independent Adverse Prognostic Marker in AML and Is Potentially Implicated in the Regulation of Ferroptosis and Immune ResponseInternational Journal of Molecular SciencesJune 2, 2023Herbert Augustus Pitts, Chi-Keung Cheng, Joyce Sin Cheung, Murphy Ka-Hei Sun, Yuk-Lin Yung, Hoi-Yun Chan, Raymond S. M. Wong, Sze-Fai Yip, Ka-Ngai Lau, Wai Shan Wong, et al.for 1 h at room temperature with 5% non-fat dry milk (Cell Signaling Technology, Danvers, MA, USA, #9999) in TBS TweenTM 20 Buffer (#28360, ThermoFisher). This was followed by incubation with primary antibodies

(13) Activating an Adaptive Immune Response with a Telomerase-Mediated Telomere Targeting Therapeutic in Hepatocellular CarcinomaMolecular cancer therapeuticsJune 1, 2023Mender I, Siteni S, Barron S, Flusche AM, Kubota N, Yu C, Cornelius C, Tedone E, Maziveyi M, Grichuk A, et al.Membranes were blocked with TBST buffer containing 5% skim milk (Cell Signaling Technology, Catalog No. 9999) for 1 hour and incubated overnight at 4°C with various primary antibodies (1:1,000). Following three

(14) FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicityCell metabolismMay 2, 2023Wieder N, Fried JC, Kim C, Sidhom EH, Brown MR, Marshall JL, Arevalo C, Dvela-Levitt M, Kost-Alimova M, Sieber J, et al.(#1704155, BioRad) according to the manufacturer’s protocol. Membranes were blocked in 5% Nonfat Dry Milk (#9999S, Cell Signaling Technology) in PBS with 0.1% Tween 20 (PBS-T). AKT and pAKT blots were blocked instead

(15) Super Carbonate Apatite-miR-497a-5p Complex Is a Promising Therapeutic Option against Inflammatory Bowel DiseasePharmaceuticals (Basel)April 19, 2023Naoto Tsujimura, Takayuki Ogino, Masayuki Hiraki, Taisei Kai, Hiroyuki Yamamoto, Haruka Hirose, Yuhki Yokoyama, Yuki Sekido, Tsuyoshi Hata, Norikatsu Miyoshi, et al.#1620177). The membranes were blocked with 5% non-fat dry milk (Cell Signaling Technology, Inc. Cat, No. #9999, Beverly, MA, USA) in TBS with Tween-20 (TBS-T; 50 mM Tris, 158 mM NaCl, 2.7 mM KCl, pH 7.5, 0.1% Tween-20)

(16) Aberrant somatic calcium channel function in cNurr1 and LRRK2-G2019S micenpj Parkinson's DiseaseApril 7, 2023Olga Skiteva, Ning Yao, Ioannis Mantas, Xiaoqun Zhang, Thomas Perlmann, Per Svenningsson, Karima Cherguifor 1 h at room temperature with 5% (w/v) fat-free dry milk (Cell Signaling, Danvers, USA, Cat. No. 9999 S) in TBS-T (Tris base 0.05 mol/L, NaCl 0.15 mol/L, tween 0.1%). Immunoblotting was carried out with

(17) FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicitybioRxiv : the preprint server for biologyFebruary 20, 2023Wieder N, Fried JC, Kim C, Sidhom EH, Brown MR, Marshall JL, Arevalo C, Dvela-Levitt M, Kost-Alimova M, Sieber J, et al.(#1704155, BioRad) according to the manufacturer’s protocol. Membranes were blocked in 5% Nonfat Dry Milk (#9999S, Cell Signaling Technology) in PBS with 0.1% Tween® 20 (PBS-T). AKT and pAKT blots were blocked instead

(18) Pyrrolyldihydropyrazino[1,2-a]indoletrione Analogue Microtubule Inhibitor Induces Cell-Cycle Arrest and Apoptosis in Colorectal Cancer CellsMolecules (Basel, Switzerland)February 17, 2023Yang DL, Qin HX, Zhang NN, Zhang YJ, Huang JH, Hu CS, Zhang XX, Li Y, He LJ.difluoride (PVDF) membranes (MilliporeSigma). The membranes were blocked with nonfat dry milk (cat no. 9999S; Cell Signaling Technology, Inc., Danvers, MA, USA) for 1 h at room temperature; afterwards, they were

(19) Age- and Sex-Dependent Behavioral and Neurochemical Alterations in hLRRK2-G2019S BAC MiceBiomoleculesDecember 27, 2022Ning Yao, Olga Skiteva, Karima Chergui1 h at room temperature with 5% (w/v) fat-free dry milk (Cell Signaling, Danvers, MA, USA, Cat. No. 9999S) in TBS-T (Tris base 0.05 mol/L, NaCl 0.15 mol/L, tween 0.1%). Immunoblotting was carried out with primary

(20) Protocol to purify recombinant inflammatory caspases and assess their catalytic activity in vitroSTAR protocolsDecember 16, 2022Devant P, Kagan JC.Diagnostics,EC-892 TEMED,Sigma,T9281-25ML Ammonium persulfate,Thermo Fisher Scientific,17874 Nonfat dry milk,Cell Signaling Technologies,9999S SOC outgrowth media,NEB,B9020 NaCl,Sigma,S9625-5KG ,, Critical commercial assays,Critical commercial

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Nonfat Dry Milk from Cell Signaling Technology

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Nonfat Dry Milk from Cell Signaling Technology

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