pCI Mammalian Expression Vector from Promega

Product Specs
ItempCI Mammalian Expression Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1731
Quantity20microg
Selectable MarkerAmp
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Ampicillin

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Characterization of two pathological gating-charge substitutions in Cav1.4 L-type calcium channelsChannels (Austin)December 31, 2023Thomas Heigl, Michael A. Netzer, Lucia Zanetti, Matthias Ganglberger, Monica L. Fernández-Quintero, Alexandra Koschakp.(Arg975Gly) and p.(Arg1299Leu); UNIPROT O60840–1) were inserted into the “pCI mammalian expression vector” (E1731, Promega) by applying splicing by overlap extension PCR. In short, the human Cav1.4 coding sequence

(2) Seizures, behavioral deficits, and adverse drug responses in two new genetic mouse models of HCN1 epileptic encephalopathyeLifeAugust 16, 2022Merseburg A, Kasemir J, Buss EW, Leroy F, Bock T, Porro A, Barnett A, Tröder SE, Engeland B, Stockebrand M, et al.experiments to express human HCN1 and mouse TRIP8b Recombinant DNA reagent,pCI (plasmid),Promega,U47119;Cat# E1731,Used for HEK293T experiments to express mouse HCN2 Recombinant DNA reagent,pmaxGFP (plasmid),Amaxa

(3) Internal Introns Promote Backsplicing to Generate Circular RNAs from Spinal Muscular Atrophy GeneGenes (Basel)June 25, 2022Diou Luo, Natalia Nikolaevna Singh, Ravindra Narayan Singhwas constructed by inserting PCR amplified products in pCI vector (Promega, Madison, WI, USA, Cat No. E1731) using cDNA and/or genomic DNA as template, as described in Supplementary Figure S1. Other expression

(4) Rixosomal RNA degradation contributes to silencing of Polycomb target genesNatureApril 7, 2022Haining Zhou et al.mutated from 5′-AGACCTAAGTTCTGTCGAA-3′ to 5′-CGGCCGAAATTTTGCAGGA-3′ and integrated into the pCI (Promega, E1731) plasmid for ectopic protein expression. For bacteria protein expression, cDNA was integrated to pGEX-6P-1

(5) Dual RNA 3’-end processing of H2A.X messenger RNA maintains DNA damage repair throughout the cell cycleNature CommunicationsDecember 1, 2021Esther Griesbach, Margarita Schlackow, William F. Marzluff, Nick J. Proudfootbacteriophage SP6 promoter sequence were cloned downstream of the T7 promoter of the pCI vector (Promega, #E1731). PCR products using T7 and SP6 forward primers were used as template for in vitro transcription. The

(6) Tau phosphorylation by glycogen synthase kinase 3β modulates enzyme acetylcholinesterase expressionJournal of NeurochemistryJune 1, 2021María‐Ángeles Cortés‐Gómez, Esther Llorens‐Álvarez, Jordi Alom, Teodoro del Ser, Jesús Avila, Javier Sáez‐Valero, María‐Salud García‐Ayllón1994); the tau triple mutant tau‐VLW also inserted into the pSG vector. The “empty” pCI vector (Cat. No. E1731: Promega) served as a negative control. We also employed a GFP‐PCI vector (Promega) as a control to

(7) Semiochemical responsive olfactory sensory neurons are sexually dimorphic and plasticeLifeNovember 24, 2020Vihani A, Hu XS, Gundala S, Koyama S, Block E, Matsunami H.the manufacturer’s guidelines. Amplified fragments were cloned into pCI expression vectors (Promega E1731) containing the first 20 residues of human rhodopsin (Rho-pCI) and were verified by sequencing. Generation

(8) IFN-γ treatment protocol for MHC-I^lo/PD-L1⁺ pancreatic tumor cells selectively restores their TAP-mediated presentation competence and CD8 T-cell priming potentialJournal for ImmunoTherapy of CancerAugust 31, 2020Katja Stifter, Jana Krieger, Leonie Ruths, Johann Gout, Medhanie Mulaw, Andre Lechel, Alexander Kleger, Thomas Seufferlein, Martin Wagner, Reinhold Schirmbecksynthesized (codon optimized) by GeneArt (Regensburg, Germany) and cloned into the pCI vector (cat. no. E1731, Promega, Mannheim, Germany) using the NheI and NotI restriction sites. All antigen modifications were

(9) Herpes Simplex Virus 1 ICP34.5 Alters Mitochondrial Dynamics in NeuronsJournal of virologyJuly 1, 2020Manivanh R, Mehrbach J, Charron AJ, Grassetti A, Cerón S, Taylor SA, Cabrera JR, Gerber S, Leib DA.viruses, the mCherry open reading frame was cloned into the multiple-cloning site of plasmid pCI (Promega E1731). The BglII-BamHI region of the resulting plasmid, which contains mCherry under the CMV immediate early

(10) A non-canonical role for the EDC4 decapping factor in regulating MARF1-mediated mRNA decayeLifeJune 8, 2020William R Brothers, Steven Hebert, Claudia L Kleinman, Marc R Fabianbackbone used for selecting transfected cells with puromycin Recombinant DNA reagent,pCI-neo,Promega,E1731,For all constructs labelled ‘LNHA’ and ‘RL’ Recombinant DNA reagent,pBABE-3xFLAG-MARF1Cterm,"Nishimura

(11) Probing Morbillivirus Antisera Neutralization Using Functional Chimerism between Measles Virus and Canine Distemper Virus Envelope GlycoproteinsVirusesJuly 27, 2019Muñoz-Alía MA, Russell SJ.containing the human elongation factor was followed by a chimeric intron (pCI vector; Promega Corp, Cat. #E1731), T7opt, HHRbz, and cloning sites were synthesized and ligated into the vector. A SV40 polyadenylation

(12) A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA SequencingCell SystemsMarch 20, 2019Eric M. Jones et al.DNA,Recombinant DNA,Recombinant DNA pCRE-Luc,Stratagene,219076 pRL-SV40,Promega,E2231 pCI,Promega,E1731 Super PiggyBac Transposase,Systems Bioscience,PB210PA-1 ,, Software and Algorithms,Software and Algorithms,Software

(13) Preproinsulin Designer Antigens Excluded from Endoplasmic Reticulum Suppressed Diabetes Development in NOD Mice by DNA VaccinationMolecular therapy. Methods & clinical developmentMarch 15, 2019Stifter K, Schuster C, Krieger J, Spyrantis A, Boehm BO, Schirmbeck R.constructs by standard cloning techniques. All constructs were cloned into the pCI vector (cat. no. E1731, Promega, Mannheim, Germany) using the NheI and NotI restriction sites. Batches of DNA were produced

(14) Endogenously Expressed Antigens Bind Mammalian RNA via Cationic Domains that Enhance Priming of Effector CD8 T Cells by DNA VaccinationMolecular TherapyMarch 1, 2019Jana Krieger, Petra Riedl, Katja Stifter, Gleyder Roman-Sosa, Thomas Seufferlein, Martin Wagner, Reinhold Schirmbeck(cat. no. E05548; NEB, Frankfurt, Germany). All sequences were cloned into the pCI vector (cat. no. E1731; Promega, Mannheim, Germany). Batches of DNA were produced in E. coli using the QIAGEN Plasmid Mega

(15) Downregulation of ERG and FLI1 expression in endothelial cells triggers endothelial-to-mesenchymal transitionPLoS geneticsNovember 1, 2018Nagai N, Ohguchi H, Nakaki R, Matsumura Y, Kanki Y, Sakai J, Aburatani H, Minami T.NM_001302153.1) or Fli1 (Gene accession: NM_008026) using pCI Mammalian Expression Vector (Promega, E1731) were used as samples. Anti-ERG [9FY] (ab136152) and Anti-FLI1 (ab15289) have specific binding activities

(16) Exploring the induction of preproinsulin-specific Foxp3(+) CD4(+) Treg cells that inhibit CD8(+) T cell-mediated autoimmune diabetes by DNA vaccinationScientific reportsJuly 11, 2016Stifter K, Schuster C, Schlosser M, Boehm BO, Schirmbeck R.synthesized by GeneArt (Regensburg, Germany). All constructs were cloned into the pCI vector (cat. no. E1731, Promega, Mannheim, Germany) using the NheI and NotI restriction sites. Batches of DNA were produced

(17) Endosomal MR1 Trafficking Plays a Key Role in Presentation of Mycobacterium tuberculosis Ligands to MAIT CellsPLoS pathogensMarch 1, 2016Harriff MJ, Karamooz E, Burr A, Grant WF, Canfield ET, Sorensen ML, Moita LF, Lewinsohn DM.28704), the 1.8kb MR1 eGFP insert was cloned into the pCI mammalian expression vector (Promega Cat. E1731) using EcoRI and KpnI restriction sites. Confirmatory sequencing of our construct revealed a missense

(18) A missing PD-L1/PD-1 coinhibition regulates diabetes induction by preproinsulin-specific CD8 T-cells in an epitope-specific mannerPloS oneJanuary 1, 2013Schuster C, Brosi H, Stifter K, Boehm BO, Schirmbeck R.synthesized by GeneArt (Regensburg, Germany). All constructs were cloned into the pCI vector (cat.no. E1731, Promega, Mannheim, Germany) using the NheI and NotI restriction sites. Batches of plasmid DNA were

(19) The Immunodominant CD8 T Cell Response to the Human Cytomegalovirus Tegument Phosphoprotein pp65_495–503Epitope Critically Depends on CD4 T Cell Help in Vaccinated HLA-A*0201 Transgenic MiceThe Journal of ImmunologySeptember 1, 2011Michael Reiser, Andreas Wieland, Bodo Plachter, Thomas Mertens, Jochen Greiner, Reinhold Schirmbecksequences were synthesized by GeneArt (Regensburg, Germany) and cloned into the pCI vector (catalog no. E1731; Promega, Mannheim, Germany) with the NheI and NotI restriction sites. Transient expression of pp65

(20) Processing in the Endoplasmic Reticulum Generates an Epitope on the Insulin A Chain that Stimulates Diabetogenic CD8 T Cell ResponsesThe Journal of ImmunologyDecember 1, 2009Helen Brosi, Michael Reiser, Tarvo Rajasalu, Andreas Spyrantis, Franz Oswald, Bernhard Otto Boehm, Reinhold Schirmbeck

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