pTARGET Mammalian Expression Vector System from Promega

Product Specs
ItempTARGET Mammalian Expression Vector System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA1410
Quantity20 reactions
Selectable Markerneomycin
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Neomycin

Specifications/Features

Advantages:
Simple PCR Cloning; Strong, Constitutive Expression; Blue/White Screening; Stable Transfectants

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) SIX1 and EWS/FLI1 co-regulate an anti-metastatic gene network in Ewing SarcomaNature CommunicationsJuly 19, 2023Connor J. Hughes, Kaiah M. Fields, Etienne P. Danis, Jessica Y. Hsu, Deepika Neelakantan, Melanie Y. Vincent, Annika L. Gustafson, Michael J. Oliphant, Varsha Sreekanth, Vadym Zaberezhnyy, et al.treated with increasing doses of Vincristine (Sigma-Aldrich, V8388) or Actinomycin D (Sigma-Aldrich, A1410) and incubated at 37 °C for 48 h. After incubation, 100ul of CellTiter-Glo 2.0 (Promega, G9243) was

(2) Cryptosporidium uses CSpV1 to activate host type I interferon and attenuate antiparasitic defensesNature communicationsMarch 16, 2023Deng S, He W, Gong AY, Li M, Wang Y, Xia Z, Zhang XT, Huang Pacheco AS, Naqib A, Jenkins M, et al.into the pTargeT™ vector using the pTargeT™ Mammalian Expression Vector System (Promega, catalogue no. A1410). Single-strand CSpV1-RNAs were synthesized using SP6/T7 Transcription Kit (Sigma-Aldrich, catalogue

(3) Quantitative trait loci on chromosomes 9 and 19 modulate AII amacrine cell number in the mouse retinaFrontiers in NeuroscienceFebruary 2, 2023Bridget Kulesh, Rachel Bozadjian, Ryan J. Parisi, Stephanie A. Leong, Amanda G. Kautzman, Benjamin E. Reese, Patrick W. KeeleyPurified amplicons were cloned into the multiple cloning site of the pTargeT Mammalian Expression Vector (#A1410; Promega), which uses a CMV enhancer/promoter to drive expression of the coding sequence and contains

(4) The tamoxifen-regulated, long non-coding RNA LINC00992 affects proliferation, migration, and expression of tamoxifen resistance-associated genes in MCF-7 breast cancer cellsWspółczesna OnkologiaJanuary 26, 2023Sebastian Graf, Johannes Haybaeck, Gerhard Behre, Thomas Kalinski, Norbert Nassinsert. Up-regulation of LINC00992 was performed via transient transfection with the vector pTarget (PN: A1410) by Promega GmbH (Walldorf, Germany), into which the cDNA of LINC00992 was previously integrated using

(5) Neurog2 regulates Isl1 to modulate horizontal cell numberDevelopmentJanuary 1, 2023Patrick W. Keeley, Pooja S. Patel, Matthew S. Ryu, Benjamin E. Reesethe brain of a 1-day-old B6/J mouse, and then cloned into the pTARGET Mammalian Expression Vector (A1410, Promega), in which the expression of Neurog2 is driven by the CMV promoter. The entire 5′UTR of the

(6) AQP4-dependent glioma cell features affect the phenotype of surrounding cells via extracellular vesiclesCell & BioscienceSeptember 7, 2022Laura Simone, Francesco Pisani, Elena Binda, Antonio Frigeri, Angelo L. Vescovi, Maria Svelto, Grazia P. Nicchiacalled AQP4-tetramers) and M23-AQP4 (also called AQP4-OAPs) coding sequences were cloned into pTarget (A1410, www.Promega.com) vectors. The previously characterized mutated form of M1-AQP4 (M23I), demonstrated

(7) Predisposition to Proinsulin Misfolding as a Genetic Risk to Diet-Induced DiabetesDiabetesNovember 1, 2021Maroof Alam, Anoop Arunagiri, Leena Haataja, Mauricio Torres, Dennis Larkin, John Kappler, Niyun Jin, Peter Arvanrabbit, or mouse 2°",Jackson ImmunoResearch,,"1:5,000" Recombinant DNA reagent,pTARGET (vector),Promega,A1410, Recombinant DNA reagent,Myc-WT proinsulin (plasmid),Liu et al.57,,Human proinsulin Commercial assay

(8) DZIP1 regulates mammalian cardiac valve development through a Cby1-β-catenin mechanismDevelopmental dynamics : an official publication of the American Association of AnatomistsOctober 1, 2021Guo L, Beck T, Fulmer D, Ramos-Ortiz S, Glover J, Wang C, Moore K, Gensemer C, Morningstar J, Moore R, et al.sequence at the C terminus using ptarget tm Mammalian Expression Vector System (Promega, Cat No: A1410). The Dzip1 mutation was incorporated through a QuickChange II XL Site‐Directed Mutagenesis Kit (Agilent

(9) Distinct states of proinsulin misfolding in MIDYCellular and molecular life sciences : CMLSAugust 1, 2021Haataja L, Arunagiri A, Hassan A, Regan K, Tsai B, Dhayalan B, Weiss MA, Liu M, Arvan P.rabbit or mouse 2°",Jackson ImmunoResearch,,(1:5000) Recombinant DNA reagent,pTARGET (vector),Promega,A1410, Recombinant DNA reagent,Myc-wild-type proinsulin (plasmid),Liu et al. PLoS One [12],,hum. proins Recombinant

(10) Ectopic expression of pericentric HSATII RNA results in nuclear RNA accumulation, MeCP2 recruitment, and cell division defectsChromosomaMarch 1, 2021Catherine C. Landers, Christina A. Rabeler, Emily K. Ferrari, Lia R. D’Alessandro, Diana D. Kang, Jessica Malisa, Safia M. Bashir, Dawn M. Carone,StrataClone PCR Cloning Kit,Agilent,Agilent:240205, Commercial assay or kit,pTargeT™ Mammalian Expression Vector System,Promega,Promega:A1410, Commercial assay or kit,iScript™ Reverse Transcription Supermix for RT-qPCR,Bio-Rad,Bio-Rad:1708841, Commercial

(11) The Role of NF-kB in the Downregulation of Organic Cation Transporter 2 Expression and Renal Cation Secretion in Kidney DiseaseFrontiers in medicineJanuary 1, 2021Han C, Zheng J, Wang F, Lu Q, Chen Q, Hu A, Visentin M, Kullak-Ublick GA, Gai Z, Chu L.transcription starting site of the SLC22A2 gene was isolated and blunt ligated into the pTargeT vector (A1410, Promega, Madison, WI, USA). The insert was then subcloned in pMCS-Luc vector (16146, ThermoFisher scientific,

(12) A non-canonical role for the EDC4 decapping factor in regulating MARF1-mediated mRNA decayeLifeJune 8, 2020William R Brothers, Steven Hebert, Claudia L Kleinman, Marc R FabiansiRNA,Dharmacon,L-008425-01-0005,SMARTpool "Peptide, recombinant protein",Actinomycin D,Sigma-Aldrich,A1410, Commercial assay or kit,GoTaq qPCR Master Mix,Promega,A6001,Reagent for all qPCR assays Commercial

(13) krCRISPR: an easy and efficient strategy for generating conditional knockout of essential genes in cellsJournal of Biological EngineeringDecember 1, 2019Bei Wang, Zishi Wang, Daqi Wang, Baolong Zhang, Sang-Ging Ong, Mingqing Li, Wenqiang Yu, Yongming Wangfor PCR-amplification. The PCR products containing gRNA targeting sites were cloned into T-vector (A1410, Promega) according to the manufacturer’s instructions for Sanger sequencing analysis. For plasmid replacement

(14) Transcriptional down-regulation of ccr5 in a subset of HIV+ controllers and their family memberseLifeApril 9, 2019Gonzalo-Gil E, Rapuano PB, Ikediobi U, Leibowitz R, Mehta S, Coskun AK, Porterfield JZ, Lampkin TD, Marconi VC, Rimland D, et al.calcium salt,Sigma,I3909, "Chemical compound, drug",Actinomycin D,Sigma. From Streptomyces sp,Cat # A1410, "Chemical compound, drug",Digitonin,Promega,G944A, Other,Power SYBR Green PCR Master Mix,ThermoFisher,Cat

(15) Inactivation of CDK12 Delineates a Distinct Immunogenic Class of Advanced Prostate CancerCellJune 14, 2018Yi-Mi Yi-Mi et al.Recombinant Proteins","Chemicals, Peptides, and Recombinant Proteins" Actinomycin D,Sigma-Aldrich,A1410–10MG RQ1 RNase-Free DNase,Promega,M6101 Superscript II Reverse Transcriptase,Invitrogen,18064–071 RNase

(16) Connexin 43 enhances paclitaxel cytotoxicity in colorectal cancer cell linesExperimental and therapeutic medicineAugust 1, 2017Wang S, Zhang S, Zhao Z, Zhang C, Yang X, Wang Y.Hercules, CA, USA). A total of 45 ng purified PCR product was inserted into 140 ng pTARGET vector (A1410; Promega Corporation, 1213 Madison, WI, USA). Constructed expression vector (0.8 µg/106 cells) was

(17) Induction of VMAT-1 and TPH-1 Expression Induces Vesicular Accumulation of Serotonin and Protects Cells and Tissue from Cooling/Rewarming InjuryPLoS ONEJanuary 12, 2012Fatemeh Talaei, Martina Schmidt, Robert H. Henninggel at 100V for 20 min (BIO-RAD, PowerPac 300). The sequences were inserted into pTARGET (Promega, A1410) and transformed into 5 HD-α E. coli. Plasmid DNA from positive colonies were isolated (nucleobond PC100,

(18) Targeting interferon regulatory factors to inhibit activation of the type I IFN response: Implications for treatment of autoimmune disordersCellular ImmunologyAugust 10, 2011Susan E. Sweeneypoly (I-C) treatment, THP-1 cells were treated with 5μg/mL actinomycin D to inhibit transcription (A1410) purchased from Sigma (St. Louis, MO), for 0.5h, 1h, 2h, 6h, or 18h. Cells were harvested using RNA

(19) Localizing the reovirus packaging signals using an engineered m1 and s2 ssRNAVirologyAugust 14, 2006Michael R. Roner, Bradley G. Steele

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