SureBeads™ Magnetic Beads System from Bio-Rad

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ItemSureBeads™ Magnetic Beads System
CompanyBio-Rad
Price Supplier Page View Company Product Page
Catalog NumberSureBeads
QuantityEA

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) RNA-Binding Proteins Regulate Post-Transcriptional Responses to TGF-β to Coordinate Function and Mesenchymal Activation of Murine Endothelial CellsArteriosclerosis, Thrombosis, and Vascular BiologyOctober 1, 2023Rhys Wardman, Merve Keles, Ihor Pachkiv, Shruthi Hemanna, Steve Grein, Jennifer Schwarz, Frank Stein, Roxana Ola, Gergana Dobreva, Matthias W. Hentze, et al.and homogenized by passing through a 21 gauge needle. Fifty microliters of Biorad Protein A magnetic Surebeads were coupled to 5 μg anti–hnRNP H1 or anti-Csde1 antibody overnight at 4 °C. Ten percent of lysate samples

(2) Customization of the translational complex regulates mRNA-specific translation to control CNS regenerationNeuronSeptember 20, 2023Schaeffer J, Vilallongue N, Decourt C, Blot B, El Bakdouri N, Plissonnier E, Excoffier B, Paccard A, Diaz JJ, Humbert S, et al.was spared for analysis. 950μl cell lysate was pre-cleared for 1h with 95μl protein G magnetic beads (SureBeads, Bio-Rad, 1614023) pre-washed 3x in lysis buffer, with end-over-end agitation. Beads were separated

(3) LRRC23 truncation impairs radial spoke 3 head assembly and sperm motility underlying male infertilitybioRxiv : the preprint server for biologySeptember 1, 2023Hwang JY, Chai P, Nawaz S, Choi J, Lopez-Giraldez F, Hussain S, Bilguvar K, Mane S, Lifton RP, Ahmad W, et al.lysates were centrifuged at 18,000 × g for 30 minutes at 4 °C and the supernatant was incubated with Surebeads™ Protein A Magnetic Bead (Bio-rad) conjugated with rabbit monoclonal DYKDDDDK antibody at RT for two

(4) An Improved Model for Circular RNA Overexpression: Using the Actin Intron Reveals High Circularization EfficiencyAdv Genet (Hoboken)September 1, 2023Feiya Li, Juanjuan Lyu, Yang Yang, Qiwei Yang, Cristian Santos, Burton B. Yangwestern blot). Immunoprecipitation Assays Immunoprecipitation assays were performed using magnetic beads (SureBeads, Cat#161‐4013, Bio‐Rad). Briefly, 100 µl magnetic beads were washed in PBS‐T (PBS + 0.1% Tween 20) and

(5) TRIM26 positively affects hepatitis B virus replication by inhibiting proteasome-dependent degradation of viral core proteinSci RepAugust 21, 2023Yuki Nakaya, Tsutomu Nishizawa, Hironori Nishitsuji, Hiromi Morita, Tomoko Yamagata, Daichi Onomura, Kazumoto Muratawith a protease inhibitor cocktail (nacalai tesque, Kyoto, Japan). The lysates were precleared using SureBeads Protein G Magnetic Beads (Bio-Rad, Hercules, CA, USA) and precleared lysates containing 1–2 mg protein

(6) Chemokine receptor CXCR7 activates Aurora Kinase A and promotes neuroendocrine prostate cancer growthThe Journal of clinical investigationAugust 1, 2023Gritsina G, Fong KW, Lu X, Lin Z, Xie W, Agarwal S, Lin D, Schiltz GE, Beltran H, Corey E, et al.protein G-conjugated magnetic beads for mouse-derived Ab and protein A-conjugated for rabbit-derived Ab (SureBeads; Bio-Rad). Bound proteins were eluted with 1.5 × sample buffer for 10 minutes at 95°C with shaking at

(7) HSPB8 frameshift mutant aggregates weaken chaperone-assisted selective autophagy in neuromyopathiesAutophagyAugust 1, 2023Tedesco B, Vendredy L, Adriaenssens E, Cozzi M, Asselbergh B, Crippa V, Cristofani R, Rusmini P, Ferrari V, Casarotto E, et al.cocktail (Sigma-Aldrich, P8340) and then centrifuged at maximum speed. For the immunoprecipitation, SureBeads Protein A Magnetic Beads (Bio-Rad, 161–4013) were used, following the manufacturers’ instructions. An

(8) LIN28B promotes cell invasion and colorectal cancer metastasis via CLDN1 and NOTCH3JCI InsightJuly 24, 2023Kensuke Sugiura, Yasunori Masuike, Kensuke Suzuki, Alice E. Shin, Nozomu Sakai, Hisahiro Matsubara, Masayuki Ohtsuka, Peter A. Sims, Christopher J. Lengner, Anil K. RustgiLIN28Bhi, Caco-2 Ctrl, DLD-1 LIN28Bhi). LIN28B ribonucleoprotein particles were immunoprecipitated using SureBeads Protein G Magnetic Beads (Bio-Rad, 161-4023) and RIP-Assay Kit (MBL, RN1001) according to the manufacturer’s

(9) Changes in SUMO-modified proteins in Epstein-Barr virus infection identifies reciprocal regulation of TRIM24/28/33 complexes and the lytic switch BZLF1PLOS PathogensJuly 6, 2023Carlos F. De La Cruz-Herrera, Michael H. Tatham, Umama Z. Siddiqi, Kathy Shire, Edyta Marcon, Jack F. Greenblatt, Ronald T. Hay, Lori Frappierp120-101) antibodies at 4°C for 16 hours with mixing. The lysate/antibody mix was added to 50 μl of SureBeads Protein A Magnetic beads (BioRad #161–4013) pre-washed in RIPA buffer, and incubated 1 hour with mixing.

(10) Oxime blot: A novel method for reliable and sensitive detection of carbonylated proteins in diverse biological systemsRedox BiologyJuly 1, 2023Romain Ladouce, Guillaume Fabien Combes, Katarina Trajković, Irena Drmić Hofman, Mladen MerćepSanta Cruz Biotechnology (Dallas, TX, USA). Acrylamide/bis-acrylamide 40% and protein A magnetic beads (SureBeads™) were obtained from Bio-Rad Laboratories (Hercules, CA, USA). N,N,N′,N′-tetramethylethylenediamine

(11) Ubiquitin Degradation of the AICAR Transformylase/IMP Cyclohydrolase Ade16 Regulates the Sexual Reproduction of Cryptococcus neoformansJournal of FungiJune 24, 2023Liantao Han, Yujuan Wu, Sichu Xiong, Tongbao Liuantibodies to ensure the correct tagged strains were obtained. Protein pull-down was performed using SureBeads™ anti-HA or anti-Flag Magnetic Beads (Bio-RAD) as described previously [24] to collect the tagged proteins,

(12) The hidden diversity of ancient bornaviral sequences from X and P genes in vertebrate genomesVirus EvolutionJune 22, 2023Bea Clarise B Garcia, Yahiro Mukai, Keizo Tomonaga, Masayuki Horieprotease inhibitor cocktail [Nacalai Tesque]) for 30 min at 4°C with rotation. In parallel, 500 μg of SureBeads Protein G magnetic beads (Bio-Rad) were incubated with 3 μg of mouse monoclonal anti-DDDDK antibody

(13) RNA Helicase DDX3 Regulates RAD51 Localization and DNA Damage Repair in Ewing SarcomabioRxivJune 15, 2023Matthew E. Randolph, Marwa Afifi, Aparna Gorthi, Rachel Weil, Breelyn A. Wilky, Joshua Weinreb, Paul Ciero, Natalie ter Hoeve, Paul J. van Diest, Venu Raman, et al.Biotechnology) or rabbit anti-DDX3 IgG (RRID:AB _2910140, Abcam, Waltham, MA, USA) were conjugated to SureBeads™ Protein G (1614023) or Protein A (1614013) Magnetic Beads (Bio-Rad Laboratories), respectively. Conjugation

(14) The Caulobacter NtrB-NtrC two-component system bridges nitrogen assimilation and cell developmentbioRxivJune 11, 2023Hunter North, Maeve McLaughlin, Aretha Fiebig, Sean Crossonadded to a final concentration of 1% (v/v). The sample was precleared via incubation with 30 μl of SureBeads Protein A magnetic agarose beads (BioRad) for 30 min at RT. The supernatant was transferred to a clean

(15) IRF7 and UNC93B1 variants in an infant with recurrent herpes simplex virus infectionJournal of Clinical InvestigationJune 1, 2023Megan H. Tucker, Wei Yu, Heather L Menden, Sheng Xia, Carl F. Schreck, Margaret I Gibson, Daniel A Louiselle, Tomi Pastinen, Nikita Raje, Venkatesh Sampathphosphatase inhibitors (MilliporeSigma). The clarified cell lysates were immunoprecipitated using protein G Surebeads (commercially available from Bio-Rad) following the manufacturer’s protocol, with a small aliquot taken

(16) Gαs slow conformational transition upon GTP binding and a novel Gαs regulatoriScienceMay 19, 2023Donghoon Ahn, Davide Provasi, Nguyen Minh Duc, Jun Xu, Leslie Salas-Estrada, Aleksandar Spasic, Min Woo Yun, Juyeong Kang, Dongmin Gim, Jaecheol Lee, et al.cells were washed with PBS and lysed with RIPA buffer in the presence of a protease inhibitor cocktail. SureBeads Protein G magnetic beads (Bio-Rad, Hercules, CA, USA) and anti-FLAG (1:1000) were added to the transferred

(17) mTORC2 interactome and localization determine aggressiveness of high-grade glioma cells through association with gelsolinScientific ReportsApril 29, 2023Naphat Chantaravisoot, Piriya Wongkongkathep, Nuttiya Kalpongnukul, Narawit Pacharakullanon, Pornchai Kaewsapsak, Chaiyaboot Ariyachet, Joseph A. Loo, Fuyuhiko Tamanoi, Trairak Pisitkun(16,000 × g for 10 min) was mixed with anti-RICTOR antibody (Abcam) overnight at 4 °C. After that, SureBeads™ Protein A magnetic beads (Bio-Rad) were mixed with the lysate for 60 min at room temperature for affinity purification

(18) R-loop landscape in mature human sperm: Regulatory and evolutionary implicationsFrontiers in GeneticsApril 17, 2023Maurice Scheuren, Jonas Möhner, Hans Zischlerpreviously described with minor modifications (Halász et al., 2017; Bou-Nader et al., 2022). Briefly, SureBeads™ Protein A Magnetic Beads (BioRad, United States) were pre-blocked with PBS containing 0.5% BSA and

(19) Regulation of c-SMAC formation and AKT-mTOR signaling by the TSG101-IFT20 axis in CD4+ T cellsCellular & Molecular ImmunologyApril 7, 2023Jiung Jeong, In Kang, Yumin Kim, Keun Bon Ku, Jang Hyun Park, Hyun-Jin Kim, Chae Won Kim, Jeongwoo La, Hi Eun Jung, Hyeon Cheol Kim, et al.The strep-tag II pooled protein samples were obtained from 1000 μg of lysates of HEK293T cells using SureBeads™ Protein A Magnetic Beads (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s protocol. Jurkat cells

(20) Derivation of a minimal functional XIST by combining human and mouse interaction domainsHuman Molecular GeneticsApril 6, 2023Maria Jose Navarro-Cobos, Suria Itzel Morales-Guzman, Sarah E L Baldry, Carolyn J BrownHNRNPK and IgG (as a negative control in a separate tube) with 650 μl of Dilution Buffer and 50 μl of Surebeads Protein G Magnetic Beads (BioRad) and left in rotation for 30 min at 4°C. Next, 300 μl of the lysate

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SureBeads Magnetic Beads Video

Protein G Magnetic Beads

Immunoprecipitation Workflow