The RealTime ready Cell Lysis Kit is designed for rapid, high-performance gene expression studies using cultured cells in two-step, real-time RT-PCR applications. The kit uses an advanced one-step protocol for lysing 3 to 30,000 cells within 5 minutes at room temperature. The resulting lysate can be used directly in cDNA synthesis, eliminating the time-consuming RNA purification step. Finally, the cDNA can be analyzed in real-time PCR experiments at any throughput (e.g., LightCycler® 480 Instrument, 96- and 384-well; LightCycler® 1536 Instrument, 1536-well).
Due to the user-friendly packaging and easy-to-use protocol, the RealTime ready Cell Lysis Kit can be easily adapted to all experimental and throughput requirements. In addition, the kit easily integrates into all manual and automated cell-based gene expression workflows.
Benefits:
* Increase convenience: Replace cumbersome multi-step RNA purification protocols with a simple one-step cell lysis procedure.
* Improve time to result: Lyse samples in just 5 minutes at room temperature without further steps.
* Reduce intermediate steps: Obtain faster, high-quality results by performing DNase treatment during cDNA synthesis.
* Benefit from broad applicability: Scale up from 3 to 30,000 cultured cells for real-time PCR applications in 96- to 1536-well formats with or without automation.
Background Information:
The RealTime ready Cell Lysis Kit is optimized when using the following downstream products for cDNA synthesis and real-time PCR analysis: Transcriptor cDNA Synthesis Kits, LightCycler® 480 DNA Masters, RealTime ready DNA Probes Masters, Universal ProbeLibrary probes, and RealTime ready assays.