Defined Keratinocyte SFM (1X) from Thermo Fisher Scientific

Product Specs
ItemDefined Keratinocyte SFM (1X)
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number10744019
Brand/TrademarkGibco™
Quantity1 unit
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures

Specifications/Features

Contains:
Defined Keratinocyte-SFM is a serum-free medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers (1,2). The growth-promoting activity of BPE has been replaced by the inclusion of growth promoting agents which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration (<0.1 mM) allowing for the isolation and culture of highly enriched populations of undifferentiated keratinocytes. Defined Keratinocyte-SFM is supplied as a kit consisting of a basal liquid medium and frozen supplement which is added at the time of use. The fully supplemented medium has a shelf-life of 10 weeks under normal use conditions. Product performance is confirmed by evaluation of human primary keratinocyte growth.

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Targeting PCSK9 reduces cancer cell stemness and enhances antitumor immunity in head and neck canceriScienceJune 16, 2023Yang QC, Wang S, Liu YT, Song A, Wu ZZ, Wan SC, Li HM, Sun ZJ.proteins" ,, PF-06446846,MedChemExpress,HY-120088A RPMI 1640 medium,ThermoFisher,C11875500BT KSFM serum-free keratinocyte medium,ThermoFisher,10744019 KGM-Gold serum-free keratinocyte medium,LONZA,00192151 hydrocortisone,MedChemExpress,HY-N0583 Recombinant mouse IL-2

(2) Abnormal downregulation of 10‐formyltetrahydrofolate dehydrogenase promotes the progression of oral squamous cell carcinoma by activating PI3K/Akt/Rb pathwayCancer MedicineMarch 1, 2023Yi Qu, Ying He, Hanjin Ruan, Lizheng Qin, Zhengxue Hanpenicillin‐streptavidin mixture and 400 ng/mL hydrocortisone. HOK cells were seeded in Keratinocyte SFM medium (10744019, Thermo Fisher Scientific). Cells were kept in a humidified incubator at 37°C with 5% CO2. LY294002

(3) SGOL2 promotes prostate cancer progression by inhibiting RAB1A ubiquitinationAging (Albany NY)December 31, 2022Tingting Lv, Dongwei He, Xiaokuan Zhang, Xiaojin Guo, Zijie Li, Aili Zhang, Bo Fan, Zhiyu WangC3010-0500) and 10% fetal bovine serum (FBS, Gibco,10091-148). RWPE-1 was cultured in Defined K-SFM (Gibco, 10744019) with 10% FBS. WPMY was cultured in DMEM (Meilunbio, MA0212) containing 10% FBS. All cell lines were

(4) CircRFWD3 promotes HNSCC metastasis by modulating miR-27a/b/PPARÎ³ signalingCell Death DiscoveryDecember 1, 2022Zihao Wei, Ying Wang, Jiakuan Peng, Honglin Li, Junjie Gu, Ning Ji, Taiwei Li, Xikun Zhou, Xin Zeng, Jing Li, et al.cells is shown in Appendix Table S3. HOK cells were cultured in a defined keratinocyte SFM medium (10744019, Thermo Fisher Scientific). UM1, UM2, HSC-3, CAL27, HN12, HN31, H413, and HEK293T cells were appropriately

(5) Chromatin conformation of human oral epithelium can identify orofacial cleft missing functional variantsInternational Journal of Oral ScienceDecember 1, 2022Yao Xiao, Shengbo Jiao, Miao He, Da Lin, Huanyan Zuo, Jiahao Han, Yonghua Sun, Gang Cao, Zhi Chen, Huan Liudifferentiation HIOEC were grown in Keratinocyte Serum Free Medium containing bovine pituitary extract (Cat No. 10744019, Lot No. 2120576; Thermo Fisher Scientific, USA) with antibiotics (100 U·mL−1 penicillin and 100 U·mL−1

(6) Gradual differentiation uncoupled from cell cycle exit generates heterogeneity in the epidermal stem cell layerNature cell biologyDecember 1, 2022Cockburn K, Annusver K, Gonzalez DG, Ganesan S, May DP, Mesa KR, Kawaguchi K, Kasper M, Greco V.ITGA6+/SCA1+/CD34− were collected in 400 μl Defined Keratinocyte serum-free medium (Thermo Fisher, catalogue number 10744019) with 1% DNaseI (Stem Cell Technologies, catalogue number 07900), loaded into Fluidigm C1 chips (Fluidigm)

(7) MicroRNA-145-5p modulates Krüppel-like factor 5 and inhibits cell proliferation, migration, and invasion in nasopharyngeal carcinomaBMC Molecular and Cell BiologyDecember 1, 2022Chien-Han Yuan, Wei-Chi Hsu, A.-Mei Huang, Ben-Chih Yuan, I.-Hung Chen, Chia-An Hsu, Rong-Feng Chen, Yih-Min Chu, Hui-Hui Lin, Hung-Lung Kepurchased from Sigma-Aldrich (SCC197). NP69 was cultured in keratinocyte serum-free medium (Gibco Cat. No. 10744019) supplemented with Keratinocyte-SFM Growth Supplement and 2% FBS. Cell transfection NPC cells were

(8) Epigenomic landscape study reveals molecular subtypes and EBV-associated regulatory epigenome reprogramming in nasopharyngeal carcinomaEBioMedicineNovember 11, 2022Larry Ka-Yue Chow, Dittman Lai-Shun Chung, Lihua Tao, Kui Fat Chan, Stewart Yuk Tung, Roger Kai Cheong Ngan, Wai Tong Ng, Anne Wing-Mui Lee, Chun Chung Yau, Dora Lai-Wan Kwong, et al.26140079) and 1% penicillin (GIBCO Cat# 10378016). NP69 was cultured in Keratinocyte SFM (GIBCO Cat# 10744019) supplemented with Bovine Pituitary Extract (BPE), 1 ng/ml epidermal growth factor (EGF), and 1% penicillin.

(9) Supply Chain Disruptions During COVID-19 Pandemic Uncover Differences in Keratinocyte Culture MediaJID InnovNovember 1, 2022Moran MC, Pope EM, Brewer MG, Beck LA.Gibco, Waltham, MA), KGM2 (number C-20211, PromoCell, Heidelberg, Germany), and defined media (number 10744019, Gibco) (Table 1). We focused on four KC assays: proliferation, differentiation marker expression, barrier

(10) Downregulation of SEPTIN5 inhibits prostate cancer progression by increasing CD8+ T cell infiltrationInternational Journal of Biological SciencesOctober 17, 2022Ming Chang, Yu He, Cong Liu, Risheng Lin, Xin Huang, Dongcuan Liang, Jian Zhang, Yi Lustreptomycin. The human prostate epithelial cell line RWPE1 was cultured using Defined Keratinocyte SFM (10744019, Gibco). All cells were maintained at 37 °C in a 5% CO2 incubator. Gene silencing Crispr sgRNAs were

(11) Identification of a Novel Cuproptosis-Related Gene Signature for Prognostic Implication in Head and Neck Squamous CarcinomasCancers (Basel)August 18, 2022Shouyi Tang, Li Zhao, Xing-Bo Wu, Zhen Wang, Lu-Yao Cai, Dan Pan, Ying Li, Yu Zhou, Yingqiang Shenhydrocortisone (500 ng/mL; MCE, HY-N0583). NOK cells were grown in defined keratinocyte-SFM (Gibco, 10744019) supplemented with Defined Keratinocyte-SFM Growth Supplement (Gibco, 10744019) and 1% penicillin–streptomycin

(12) Phenformin Down-Regulates c-Myc Expression to Suppress the Expression of Pro-Inflammatory Cytokines in KeratinocytesCellsAugust 5, 2022Liu G, Li D, Zhang L, Xu Q, Zhuang D, Liu P, Hu L, Deng H, Sun J, Wang S, et al.washed to obtain the cell pellets. The cell pellets were resuspended in the culture medium K-SFM (Cat. 10744019, Gibco, Grand Island, NY, USA) supplemented with 100 mg/mL streptomycin and 100 U/mL penicillin. Human

(13) Aristolochic acid I induces proximal tubule injury through ROS/HMGB1/mt DNA mediated activation of TLRsJournal of cellular and molecular medicineAugust 1, 2022Upadhyay R, Batuman V.flasks/6‐well plates (Corning) and incubated at 37°C with 5% CO2 in keratinocyte serum‐free medium (catalogue 10744019) (Gibco, Invitrogen, Thermo Fisher Scientific) supplemented with recombinant human epidermal growth

(14) 422 Keratinocyte media differences uncovered during COVID-19 supply shortagesJournal of Investigative DermatologyAugust 1, 2022E.M. Pope, M.C. Moran, M.G. Brewer, L.A. Beckacross three culture medias: KSFM (Gibco, #17005042), KGM2 (PromoCell, #C-20211), and Defined (Gibco, #10744019). We observed qualitative and quantitative differences in proliferation among KC grown in the different

(15) Aurora-A/FOXO3A/SKP2 axis promotes tumor progression in clear cell renal cell carcinoma and dual-targeting Aurora-A/SKP2 shows synthetic lethalityCell death & diseaseJuly 13, 2022Li P, Chen T, Kuang P, Liu F, Li Z, Liu F, Wang Y, Zhang W, Cai X.bovine serum and 1% penicillin-streptomycin. The HK-2 cell line was cultured in KSFM medium (Gibco, #10744019). And all cells cultured at 37 °C in a humidified atmosphere containing 5% CO2. All cell lines were

(16) Construction of Tissue-Engineered Bladder Scaffolds with Composite BiomaterialsPolymers (Basel)June 29, 2022Wenjiao Li, Na Qi, Tingting Guo, Chao Wang, Ziwei Huang, Zhouyuan Du, Dingwen Xu, Yin Zhao, Hong Tianat the bottom was used as a control group. The 3: 1 DMEM and keratinocyte serum-free medium (KSFM; 10744019, Thermo Fisher, Waltham, MA, USA) was a special medium in which DMEM contains 10% FBS and growth factor

(17) FBXW2 inhibits prostate cancer proliferation and metastasis via promoting EGFR ubiquitylation and degradationCellular and molecular life sciences : CMLSMay 2, 2022Zhou T, Chen T, Lai B, Zhang W, Luo X, Xia D, Fu W, Xu J.routinely cultured in RPMI-1640 medium (#21870076, Gibco). RWPE cell line was maintained in KSFM medium (#10744019, Gibco). 293 T cells were grown in DMEM medium (#11965092, Gibco). All mediums were supplemented with

(18) GIPC2 interacts with Fzd7 to promote prostate cancer metastasis by activating WNT signalingOncogeneApril 29, 2022Liang Wang, Jiayi Wang, Xiaolin Yin, Xin Guan, Ying Li, Chenqi Xin, Jing Liupenicillin–streptomycin (10378016, Gibco). RWPE-1 cells were maintained in Keratinocyte-SFM medium (10744019, Gibco). All cells were cultured in a humidified incubator (Heracell 2401, Thermo Fisher) at 37 °C with

(19) Differentiation of Human Induced Pluripotent Stem Cells into KeratinocytesCurr ProtocApril 1, 2022Koch PJ, Webb S, Gugger JA, Salois MN, Koster MI.SFM (DKSFM) 1 vial Defined Keratinocyte-SFM Growth Supplement (ThermoFisher Scientific, cat. no. 10744019 1% Penicillin-Streptomycin (5,000 U/ml; ThermoFisher Scientific, cat. no.15070063) Store up to 1

(20) Stabilization of p18 by deubiquitylase CYLD is pivotal for cell cycle progression and viral replicationnpj Precision OncologyDecember 1, 2021Yueshuo Li et al.Hong Kong.Cells were cultured in a 1:1 mixture of Defined Keratinocyte-SFM and EpiLife medium (Cat: 10744019 and MEPI500CA, Gibco). Cells were cultured in 37 °C incubators with 5% CO2. Reagents and antibodies MG132

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Defined Keratinocyte SFM (1X) from Thermo Fisher Scientific

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Thermo Fisher Scientific

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Defined Keratinocyte SFM (1X) from Thermo Fisher Scientific

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