KnockOut Serum Replacement from Thermo Fisher Scientific

Product Specs
ItemKnockOut Serum Replacement
CompanyThermo Fisher Scientific
Price Pricing Info Get Pricing Get Quote for this Product
Catalog Number10828028
Quantity500mL
Brand/TrademarkGibco™
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Protocol for generating in vitro glioma models using human-induced pluripotent- or embryonic-stem-cell-derived cerebral organoidsSTAR ProtocolsSeptember 1, 2023Yilin Feng, Honghui Zheng, Jiyuan Tang, Xing Li, Ruilin Tian, Shaohua Ma11330032 D-PBS,Gibco,Cat# 14190144 Neurobasal medium,Gibco,Cat# 21103049 KnockOut Serum Replacement,Gibco,Cat# 10828028 GlutaMAX supplement,Gibco,Cat# 35050061 MEM non-essential amino acids,Gibco,Cat# 11140050 Mtesr Plus,Stem Cell Technologies

(2) An engineered Sox17 induces somatic to neural stem cell fate transitions independently from pluripotency reprogrammingSci AdvAugust 25, 2023Mingxi Weng, Haoqing Hu, Matthew S. Graus, Daisylyn Senna Tan, Ya Gao, Shimiao Ren, Derek Hoi Hang Ho, Jakob Langer, Markus Holzner, Yuhua Huang, et al.KSR-mESC medium: high-glucose DMEM supplemented with 15% knockout serum replacement (KSR, Gibco, no. 10828028), 1% GlutaMAX, 1% NEAA, 1% penicillin/streptomycin, 0.1 mM β-mercaptoethanol, and LIF (10 ng/ml). Developed

(3) Extravillous trophoblast cell lineage development is associated with active remodeling of the chromatin landscapeNature communicationsAugust 10, 2023Varberg KM, Dominguez EM, Koseva B, Varberg JM, McNally RP, Moreno-Irusta A, Wesley ER, Iqbal K, Cheung WA, Schwendinger-Schreck C, et al.μM A83-01 (04-0014, Reprocell, Beltsville, MD), 2.5 μM Y27632, 4% KnockOut Serum Replacement (KSR, 10828028, Thermo-Fisher), and 2% Matrigel® (CB-40234, Thermo-Fisher). On day 3 of EVT cell differentiation, the

(4) Rhox6 regulates the expression of distinct target genes to mediate mouse PGCLC formation and ESC self-renewalCell & bioscienceAugust 8, 2023Li X, Chen P, Ji J, Duan Q, Cao J, Huang R, Ye SD.20 ng/ml Activin A (C678, Novoprotein, China), 12 ng/ml bFGF (C044, Novoprotein, China) and 1% KSR (10828028, Invitrogen) to induce EpiLCs. Two days later, 2 × 105 EpiLCs were exposed to PGCLC-inductive medium

(5) Spatially resolved multiomics of human cardiac nichesNatureJuly 27, 2023Kazumasa Kanemaru, James Cranley, Daniele Muraro, Antonio M. A. Miranda, Siew Yen Ho, Anna Wilbrey-Clark, Jan Patrick Pett, Krzysztof Polanski, Laura Richardson, Monika Litvinukova, et al.(Life Technologies, A1217702) and replated at a density of 2 × 106 per well in RPMI/B27, 10% KOSR (Thermo Fisher, 10828028) and 10 μM Y-27632 dihydrochloride. The cells were then cultured in RPMI/B27, and media were changed

(6) Internet-connected cortical organoids for project-based stem cell and neuroscience educationbioRxivJuly 20, 2023Matthew A.T. Elliott, Hunter E. Schweiger, Ash Robbins, Samira Vera-Choqqueccota, Drew Ehrlich, Sebastian Hernandez, Kateryna Voitiuk, Jinghui Geng, Jess L. Sevetson, Yohei M. Rosen, et al.Medium (Thermo Fisher Scientific # 11710035), 10% Knockout Serum Replacement (Thermo Fisher Scientific # 10828028), 0.1 mM MEM Non-Essential Amino Acids (Thermo Fisher Scientific # 11140050), 1 mM Sodium Pyruvate (Millipore

(7) Characterising the RNA-binding protein atlas of the mammalian brain uncovers RBM5 misregulation in mouse models of Huntington's diseaseNature communicationsJuly 19, 2023Mullari M, Fossat N, Skotte NH, Asenjo-Martinez A, Humphreys DT, Bukh J, Kirkeby A, Scheel TKH, Nielsen ML.containing 9.5 mL DMEM/F12 (ThermoFisher, #10565018) and 0.5 mL KnockOut Serum Replacement (ThermoFisher, #10828028) and counted using a Neubauer counting chamber. For neural induction, hESC were plated on Laminin-111

(8) Modulation of neuronal activity in cortical organoids with bioelectronic delivery of ions and neurotransmittersbioRxivJuly 19, 2023Yunjeong Park, Sebastian Hernandez, Cristian O. Hernandez, Hunter E. Schweiger, Houpu Li, Kateryna Voitiuk, Harika Dechiraju, Nico Hawthorne, Elana M. Muzzy, John A. Selberg, et al.Medium (Thermo Fisher Scientific # 11710035), 10% Knockout Serum Replacement (Thermo Fisher Scientific # 10828028), 0.1 mM MEM NonEssential Amino Acids (Thermo Fisher Scientific # 11140050), 1 mM Sodium Pyruvate (Millipore

(9) Scalable projected Light Sheet Microscopy for high-resolution imaging of living and cleared samplesbioRxiv : the preprint server for biologyJuly 18, 2023Chen Y, Chauhan S, Gong C, Dayton H, Xu C, De La Cruz ED, Datta MS, Leong KW, Dietrich LEP, Tomer R.induction medium (NIM) daily, containing DMEM/F12 medium, 20% Knockout Serum Replacement (KSR, Gibco, 10828028), 1% Minimum Essential Medium non-essential amino acids (MEM-NEAA, Gibco, 11140050), 0.1 mM 2-mercaptoethanol

(10) Derivation of Human Extraembryonic Mesoderm-like Cells from Primitive EndodermInternational Journal of Molecular SciencesJuly 12, 2023Karin Farkas, Elisabetta Ferrettidissociated with TrypLE Select and frozen in KnockOut Serum Replacement (Thermo Fisher Scientific, 10828028) with 10% DMSO. Single-cell RNAseq was performed by Single Cell Discoveries (Utrecht, The Netherlands).

(11) Derivation of embryonic stem cells from wild-derived mouse strains by nuclear transfer using peripheral blood cellsScientific ReportsJuly 10, 2023Naomi Watanabe, Michiko Hirose, Ayumi Hasegawa, Keiji Mochida, Atsuo Ogura, Kimiko InoueThe zona-free blastocysts were cultured with KnockOut-DMEM including 15% Knockout Serum Replacement (10828028, Thermo Fisher Scientific K.K., Massachusetts, USA), 1 × Non-Essential Amino Acid (11140050, Thermo Fisher Scientific

(12) Inferring regulators of cell identity in the human adult pancreasNAR Genomics and BioinformaticsJuly 10, 2023Lotte Vanheer, Federica Fantuzzi, San Kit To, Andrea Schiavo, Matthias Van Haele, Tessa Ostyn, Tine Haesen, Xiaoyan Yi, Adrian Janiszewski, Joel Chappell, et al.(SIGMA #A7089) for 8 min at 37 °C after which 50% volume of KnockOut Serum Replacement (ThermoFisher #10828028) was added to stop the reaction. After centrifugation at 400 g for 5 min at room temperature, cells

(13) A stem cell zoo uncovers intracellular scaling of developmental tempo across mammalsCell stem cellJuly 6, 2023Lázaro J, Costanzo M, Sanaki-Matsumiya M, Girardot C, Hayashi M, Hayashi K, Diecke S, Hildebrandt TB, Lazzari G, Wu J, et al.Azide,Sigma-Aldrich,Cat# 71290-10G Laminin,Amsbio,Cat# AMS.892 021 Knockout serum replacement,Gibco,Cat# 10828028 Geltrex,Thermo Fisher Scientific,Cat# A1413302 Matrigel,Corning,Cat# 356231 DMEM-F12,Gibco,Cat# 11320033 mTESR1,STEM CELL Technologies

(14) Lipid droplet-associated lncRNA LIPTER preserves cardiac lipid metabolismNature cell biologyJuly 1, 2023Han L, Huang D, Wu S, Liu S, Wang C, Sheng Y, Lu X, Broxmeyer HE, Wan J, Yang L.medium containing Dulbecco’s modified Eagle medium (DMEM)/F12 (Gibco, 11330032), 20% KSR (Invitrogen, 10828028), penicillin/streptomycin (P/S; Gibco, 15140163), GlutaMAX Supplement (Gibco, 35050061), non-essential

(15) Particulate matter 10 exposure affects intestinal functionality in both inflamed 2D intestinal epithelial cell and 3D intestinal organoid modelsFrontiers in ImmunologyJune 26, 2023Ye Seul Son, Naeun Son, Won Dong Yu, Aruem Baek, Young-Jun Park, Moo-Seung Lee, Seon-Jin Lee, Dae-Soo Kim, Mi-Young SonGlutaMAX (Gibco, 35050061), 0.1% β-mercaptoethanol (Gibco, 21985023), 10% serum replacement (SR; Gibco, 10828028), and 8 ng/ml basic fibroblast growth factor (R&D Systems, Minneapolis, MN, USA, 4114-TC)]. The medium

(16) Generation of a Well-Characterized Homozygous Chromodomain-Helicase-DNA-Binding Protein 4G1003D Mutant hESC Line Using CRISPR/eCas9 (ULIEGEe001-A-1)International Journal of Molecular SciencesJune 23, 2023Ilyas Chohra, Subhajit Giri, Brigitte Malgrangecultured in suspension using EB media [Knock-out DMEM (Gibco™ #10829018), 20% KnockOut™ Serum (Gibco™ #10828028), 2 mM GlutaMax (Gibco™ #35050061), 1% PenStrep (Gibco™ #15140122), 0.1 mM NEAA (Gibco™ #11140050),

(17) Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathyNature CommunicationsJune 22, 2023Markus Grosch, Laura Schraft, Adrian Chan, Leonie Küchenhoff, Kleopatra Rapti, Anne-Maud Ferreira, Julia Kornienko, Shengdi Li, Michael H. Radke, Chiara Krämer, et al.TrypLE10x (A1217701, ThermoFisher) and RPMI+Insulin supplemented with 10% knock-out serum replacement (10828028, ThermoFisher) and 1.66 μM Thiazovivin (72252, StemCell Technologies). One day after passaging, the

(18) Molecular and cellular evidence for the impact of a hypertrophic cardiomyopathy-associated RAF1 variant on the structure and function of contractile machinery in bioartificial cardiac tissuesCommunications BiologyJune 21, 2023Saeideh Nakhaei-Rad, Fereshteh Haghighi, Farhad Bazgir, Julia Dahlmann, Alexandra Viktoria Busley, Marcel Buchholzer, Karolin Kleemann, Anne Schänzer, Andrea Borchardt, Andreas Hahn, et al.frozen at −20 °C: DMEM/F12 (Gibco, 10565018) supplemented with 15% Knock-out serum replacement (Gibco, 10828028) and 1x non-essential amino acids, 5 ng/mL bFGF (Peprotech, 100-18B-1MG) and 0.1 mM ß-mercaptoethanol

(19) WDR5 is required for DUX4 expression and its pathological effects in FSHD muscular dystrophyNucleic Acids ResearchJune 9, 2023Emanuele Mocciaro, Roberto Giambruno, Stefano Micheloni, Filippo M Cernilogar, Annapaola Andolfo, Cristina Consonni, Maria Pannese, Giulia Ferri, Valeria Runfola, Gunnar Schotta, et al.Sigma-Aldrich, #D9785) supplemented with 20% knockout serum replacement (KOSR, Thermo Fisher Scientific, #10828028), 3.151 g/l glucose, 10 mM MEM non-essential amino acids (Thermo Fisher Scientific, #11140050), 100

(20) Combined lineage tracing and scRNA-seq reveals unexpected first heart field predominance of human iPSC differentiationeLifeJune 7, 2023Francisco Xavier Galdos, Carissa Lee, Soah Lee, Sharon Paige, William Goodyer, Sidra Xu, Tahmina Samad, Gabriela V Escobar, Adrija Darsha, Aimee Beck, et al.resuspended in 4% PFA for 10 min and were rinsed with a 5% KnockOut Serum Replacement (Thermo Fisher Cat. 10828028) solution in 1 x PBS. Cells were permeabilized in a 0.5% Saponin (Sigma Cat. S7900) solution containing

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KnockOut Serum Replacement from Thermo Fisher Scientific

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KnockOut Serum Replacement from Thermo Fisher Scientific

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