STEMium is a serum-free medium designed for optimal growth of human embryonic stem cells and induced pluripotent stem cells under feeder-free conditions. It is a sterile, liquid medium containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals.
The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/ 95% air. STEMium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively promotes growth of normal human embryonic stem cells and induced pluripotent stem cells in vitro. This medium should be used in conjunction with Geltrex or BD Matrigel for feeder-free culture conditions.
STEMium consists of 500 ml of STEMium basal medium and 10 ml of StemGS® 50X human pluripotent stem cell growth supplement (Cat. No.5852).