Description
EMD Millipore‘s KOD DNA enzyme polymerases are specially formulated to meet the challenges of high fidelity PCR. They enable accurate, fast and high yielding amplification of long PCR fragments. These polymerases are optimized to efficiently amplify from crude samples and from challenging DNA templates (with high GC/TA content). Isolated from the extreme thermophile Thermococcus kodakaraensis, these enzymes exhibit higher processivity and fidelity than conventional enzymes enabling amplification with unmatched speed and fidelity.
• KOD DNA Polymerase is a high fidelity thermostable polymerase that amplifies target DNA with superior accuracy and yield. The enzyme’s 3´-> 5´exonuclease-dependent proofreading activity results in one of the lowest PCR mutation frequencies amongst DNA polymerases. The enhanced elongation rate (5x better than Pfu DNA polymerase) and processivity (10 to 15 times higher than Pfu), result in highly accurate and robust yields in short reaction times. The enzyme generates blunt-ended PCR products.
• KOD Hot Start is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3´→ 5´ exonuclease activities at ambient temperatures.KOD Hot Start combines the high fidelity, fast extension speed, and outstanding processivity of KOD with the high specificity of an antibody-mediated hot start. In addition, KOD Hot start amplifies up to 12kb genomic DNA templates and up to 21kb from plasmid or lambda DNA. Primer degradation during setup at room temperature is effectively inhibited due to blocked exonuclease activity which is restored after heating. KOD Hot Start Master Mix is a ready-to-use 2x mixture optimized for convenient high-fidelity PCR.
• KOD-XL is a blend of the native KOD enzyme and a mutant form deficient in exonuclease activity. It is designed for reliable amplification of long targets or crude samples, for multiplex PCR and excellent incorporation of derivatized dNTPs. This enzyme blend will give a mixture of blunt and overhanging 3’-dA ended products
• KOD XtremeTM is formulated for the most difficult targets allowing amplification from minimally processed blood and lysates from cells or animal tissues (eg mouse tails), plants or microbial extracts (e.g. yeast or fungi). This enzyme efficiently amplifies long templates from plasmid (up to 40 kb) or genomic (24 kb) DNA (see image). In addition, the enzyme will amplify DNA templates with up to 90% GC content. Collectively, the KOD Polymerase family is uniquely positioned for accurate quick PCR turnaround and discovery from the most complex targets.