Description
JustPURE “Good” Buffers
- Ultrapure zwitterionic buffers
- Optimized for research in cellular and molecular biology
- Low interference with biological reactions
Zwitterionic buffers possessing both positive and negative charges were described in 1966 by Good and co-workers as being suitable for work with biological molecules. Popularly known today as “Good” buffers, these organic buffers have several advantages compared to traditional inorganic buffering systems such as phosphate, borate, and bicarbonate. “Good” buffers approach the “ideal” buffer state by having pKa values at or near physiological pH and minimal interference with biological processes. JustPURE “Good” buffers from Fisher BioReagents have very high purity (assay > 99%) and only trace amounts of metal ions, which makes them useful for applications requiring tight control of elemental content. (Reference: Good, N.E., et al. (1966) Hydrogen Ion Buffers for Biological Research. Biochemistry 5(2): 467-477)
Advantages
- pKa values mostly independent of temperature and concentration
- High water solubility
- Chemically and enzymatically stable
- Minimal permeability to biological membranes
- Limited effect on biochemical reactions
- Minimal absorption in spectral range 240 to 700nm
Applications
- High efficiency transfection of mammalian cells
- Gel electrophoresis of RNA
- Protein isolation applications
- Cell cultures and enzyme assays
- Bioanalytical methods such as IEF, 2-D electrophoresis, SDS-PAGE