ScanLater™ Western Blot Detection System from Molecular Devices LLC

Product Specs
ItemScanLater™ Western Blot Detection System
CompanyMolecular Devices LLC
Price Get Pricing Get Quote for this Product
Catalog Number0200-7027
SoftwareSoftMax® Pro Software
ApplicationsProtein identification and quantitation, Epitope mapping, Amino acid composition and sequence analysis, Spots imprinting, Phosphoprotein endogenous or exogenous expression, Protein resillience, Structure domain analysis, Protein expression, Protein content in serum, QC elimination of albumin and LgG in serum, Protein expression the the cell cycle
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Molecular Devices LLC

3860 N First Street

San Jose, California 95134

United States

Phone: 408-747-1700

Company Profile

Website: http://www.moleculardevices.com/

(1) Spike-protein proteolytic antibodies in COVID-19 convalescent plasma contribute to SARS-CoV-2 neutralizationCell Chemical BiologyJuly 20, 2023Scott A. McConnell, Jaiprasath Sachithanandham, Nathan J. Mudrak, Xianming Zhu, Parsa Alba Farhang, Radames J.B. Cordero, Maggie P. Wear, Janna R. Shapiro, Han-Sol Park, Sabra L. Klein, et al.ChemiDoc Imaging System (BioRad). For Europium detection, membranes were imaged using the time-resolved fluorescence ScanLater Western Blot protocol on the SpectraMax iD5 (Molecular Devices). Indirect enzyme-linked immunosorbent assays

(2) RNAseq reveals extensive metabolic disruptions in the sensitive SF-295 cell line treated with schweinfurthinsScientific ReportsDecember 1, 2022J. S. Weissenrieder, J. D. Weissenkampen, J. L. Reed, M. V. Green, C. Zheng, J. D. Neighbors, D. J. Liu, Raymond J. Hohlintensity of color38,39. Western blotting Western blotting was carried out with the Molecular Devices ScanLater system (San Jose, CA), as described previously40. Briefly, cells were harvested in RIPA buffer with

(3) The PI3K inhibitor pictilisib and the multikinase inhibitors pazopanib and sorafenib have an impact on Rac1 level and migration of medulloblastoma in vitroJournal of Cellular and Molecular MedicineDecember 1, 2022Leonie F. Schoen, Rogerio B. Craveiro, Torsten Pietsch, Thomas Moritz, Anja Troeger, Silvia Jordans, Dagmar Dilloo(Bio‐Rad). Blots from Figure 1D were analysed with the SpectraMax i3X (Molecular Devices) equipped with a ScanLater module in high resolution scan. Densitometry of Western blots was performed using ImageJ 1.53c. 2.9

(4) Activation of glucagon-like peptide-1 receptors reduces the acquisition of aggression-like behaviors in male miceTranslational psychiatryOctober 13, 2022Vestlund J, Zhang Q, Shevchouk OT, Hovey D, Sebastian L, Westberg L, Jerlhag E.Country)",Dilution/incubation TPH2,"Rabbit anti-TPH2 LS‑C346253, Lifespan Bio, Seattle, WA, USA",1:500 in 5% skim milk/ overnight,"Scanlater Eu-labeled Goat Anti- Rabbit lgG R8029 Molecular Devices, Sunnyvale, CA, USA",1:5000 in 5% skim milk/

(5) Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsBiochimica et biophysica acta. Molecular and cell biology of lipidsJune 1, 2022Suliman M, Case KC, Schmidtke MW, Lazcano P, Onu CJ, Greenberg ML.(Molecular Devises, USA) was used, and membranes were imaged using a SpectraMax i3x plate reader with the ScanLater module (Molecular Devices, San Jose, CA). ImageJ was used to quantify the intensity of bands, which

(6) Effect of Structural Changes Induced by Deletion of 54FLRAPSWF61 Sequence in αB-crystallin on Chaperone Function and Anti-Apoptotic ActivityInternational Journal of Molecular SciencesOctober 5, 2021Sundararajan Mahalingam, Srabani Karmakar, Puttur Santhoshkumar, Krishna K. Sharmaincubated with primary protein-specific antibodies overnight at 4 °C. The membranes were incubated with the ScanLater Eu-labeled anti-rabbit or anti-mouse secondary antibodies (Molecular Devices) for 2 h. Blots were washed,

(7) Valproate activates the Snf1 kinase in Saccharomyces cerevisiae by decreasing the cytosolic pHJournal of Biological ChemistryOctober 1, 2021Michael Salsaa, Kerestin Aziz, Pablo Lazcano, Michael W. Schmidtke, Maureen Tarsio, Maik HÃ¼ttemann, Christian A. Reynolds, Patricia M. Kane, Miriam L. Greenbergdetected using a SpectraMax i3x multimode microplate reader with MiniMax 300 imaging cytometer and ScanLater Western blotting cartridge (Molecular Devices). Membranes were visualized with the SoftMax Pro software. ATP

(8) Antipsychotic drugs elicit cytotoxicity in glioblastoma multiforme in a calcium-dependent, non-D(2) receptor-dependent, mannerPharmacology research & perspectivesMay 1, 2021Weissenrieder JS, Reed JL, Moldovan GL, Johnson MT, Trebak M, Neighbors JD, Mailman RB, Hohl RJ.experiment). 2.6 Immunoblotting Western blot analysis was carried out as described previously using the ScanLater (Molecular Devices, San Jose, CA, USA) system. 13 Membranes were incubated with primary antibodies

(9) Effects of sub-chronic amylin receptor activation on alcohol-induced locomotor stimulation and monoamine levels in micePsychopharmacology (Berl)November 1, 2020Aimilia Lydia Kalafateli, Cajsa Aranäs, Elisabet Jerlhagfinal secondary antibody solutions were as follows: 1:5000 in TBS-T of Eu-labelled goat anti-mouse ScanLater™ (Molecular Devices, San Jose, CA, USA) was used against a-COXIV, and 1:5000 in TBS-T of Eu-labelled

(10) Low dose HSP90 inhibition with AUY922 blunts rapid evolution of metastatic and drug resistant phenotypes induced by TGF-β and paclitaxel in A549 cellsBiomedicine & PharmacotherapySeptember 1, 2020Nickolas A. Bacon, Isabel Larre, Abdalla A. Lawag, Carlen Merritt, Mackinzie Smith, Matthew Rosolen, Vincent E. Sollars(Fitzgerald Industries®, cat# 10R-G109a). Species-specific europium-conjugated secondary antibody (ScanLater goat anti mouse; part#: R7562; Molecular Devices®) was used. Afterward, membranes were washed with TBS-T

(11) The Dopamine D2 Receptor Contributes to the Spheroid Formation Behavior of U87 Glioblastoma CellsPharmacologyJanuary 1, 2020Weissenrieder JS, Reed JL, Green MV, Moldovan GL, Koubek EJ, Neighbors JD, Hohl RJ.spheroid counts. Immunoblotting Western blotting was performed as previously described [22] using the ScanLater (Molecular Devices, San Jose, CA, USA) visualization system, and 4–12% bis/tris gels (Life Technologies, Carlsbad,

(12) IODVA1, a guanidinobenzimidazole derivative, targets Rac activity and Ras-driven cancer modelsPloS oneJanuary 1, 2020Gasilina A, Premnauth G, Gurjar P, Biesiada J, Hegde S, Milewski D, Ma G, Kalin TV, Merino E, Meller J, et al.processed with ECL (film or Bio-Rad chemiluminescent system) or imaged on the SpectraMax i3 platform with ScanLater module (Molecular Devices). Retrovirus production and transduction pBabe puro HRasG12V plasmid was

(13) Unbiased peptoid combinatorial cell screen identifies plectin protein as a potential biomarker for lung cancer stem cellsScientific ReportsDecember 1, 2019Aaron C. Raymond, Boning Gao, Luc Girard, John D. Minna, D. Gomika Udugamasooriyawere transferred to a nitrocellulose membrane. The membrane was then blotted and visualized using the ScanLater Western Blot Kit (Molecular Devices, Sunnyvale, CA) as follows: the membrane is blocked for 1 hour in

(14) Dietary advanced glycation end-product consumption leads to mechanical stiffening of murine intervertebral discsDisease Models & MechanismsDecember 1, 2018Divya Krishnamoorthy, Robert C. Hoy, Devorah M. Natelson, Olivia M. Torre, Damien M. Laudier, James C. Iatridis, Svenja Illien-JüngerCA, USA) for 1 h at room temperature. After washing, the membranes were dried and scanned using the ScanLater (Molecular Devices) Western Blot cartridge in a SpectraMax i3 system (Molecular Devices). Membranes

(15) IDH2 Deficiency Aggravates Fructose-Induced NAFLD by Modulating Hepatic Fatty Acid Metabolism and Activating Inflammatory Signaling in Female MiceNutrientsMay 27, 2018Jeong Hoon Pan et al.primary antibodies for 12 h at 4 ◦ C. Subsequently, the membranes were washed and incubated with the ScanLater EU-labeled secondary antibodies (Molecular Devices) for 1 h at room temperature. The protein bands were

(16) Myosin II–interacting guanine nucleotide exchange factor promotes bleb retraction via stimulating cortex reassembly at the bleb membraneMolecular Biology of the CellMarch 1, 2018Meng Jiao, Di Wu, Qize Weiusing a Trans-Blot semidry electrophoretic transfer cell (Bio-Rad). The membrane was then blocked in ScanLater 1X blocking buffer (Molecular Devices) for 1 h at 22.5°C and incubated with primary antibodies for 12

(17) Serine 392 phosphorylation modulates p53 mitochondrial translocation and transcription-independent apoptosisCell Death & DifferentiationJanuary 1, 2018Cédric Castrogiovanni, Béranger Waterschoot, Olivier De Backer, Patrick Dumontalso measured. (d) Semi-quantitative western blot analysis for the expression of total p53 using the ScanLater western blot technology (Molecular Devices). HCT-116 p53 wt and S392A cells were treated or not (NT,

(18) Antioxidant and Anti-Inflammatory Activities of a Natural Compound, Shizukahenriol, through Nrf2 ActivationMoleculesSeptember 2, 2015Jong-Hyun Park, Ji Won Choi, Eun Ji Ju, Ae Nim Pae, Ki Duk ParkBiotechnology, Inc., 1:1000) at 4 °C. Then, membranes were incubated for 1 h at room temperature with ScanLater EU-labeled secondary antibodies (Molecular Devices, Sunnyvale, CA, USA, 1:10,000 dilution in 4% skim

(19) Identification of the GTPase-activating protein DEP domain containing 1B (DEPDC1B) as a transcriptional target of Pitx2Experimental Cell ResearchApril 10, 2015Di Wu, Xiaoxi Zhu, Kevin Jimenez-Cowell, Alexander J. Mold, Christopher C. Sollecito, Nicholas Lombana, Meng Jiao, Qize WeiSuperSignal West Pico Luminol/Enhancer solution (Pierce). Some of the immunoblots were processed with a ScanLater kit and then scanned by a SpectraMax i3 according to the manufacturer’s instructions (Molecular Devices).

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