BD FACSCelesta™ Flow Cytometer from BD Biosciences

Product Specs
ItemBD FACSCelesta™ Flow Cytometer
CompanyBD Biosciences
Price Get Pricing Get Quote for this Product
Catalog NumberBD FACSCelesta
QuantityEA
DetectionFSC, SSC, up to 12 fluorescence colors
Abs / Exc488 nm (Blue), 640 nm (Red), 405 nm (Violet), 561 nm (Yellow/Green), 355 nm (UV)
Sample Type5 mL (12 x 75 mm) tubes, 96- or 384-well high-throughput sampler option
Voltage100 to 230 VAC and 50 or 60 Hz
Throughput15 min per microtiter plate in high-throughput mode using a 2-second acquisition; 44 minutes in standard mode using a 10-second acquisition

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BD Biosciences

2350 Qume Drive

San Jose, California 95131

United States

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Website: http://www.bdbiosciences.com/

(1) Structure-based engineering of a novel CD3ε-targeting antibody for reduced polyreactivitymAbsDecember 31, 2023Catherine Y Liu, Cory L Ahonen, Michael E Brown, Ling Zhou, Martin Welin, Eric M Krauland, Robert Pejchal, Paul F Widboom, Michael B BattlesPBSF (500 g for 5 min at 4°C) and resuspended in 60 µl PBSF before analyzing 30 µl per sample on a BD FACSCelesta™ Cell Analyzer (BD Biosciences). Jurkat (NFAT-luc) T cell activation bioassay Jurkat T cells genetically

(2) Stereotactic Ablative Radiation Therapy in 3 Fractions Induces a Favorable Systemic Immune Cell Profiling in Prostate Cancer PatientsOncoImmunologyDecember 31, 2023Belinda Palermo, Marta Bottero, Mariangela Panetta, Adriana Faiella, Isabella Sperduti, Serena Masi, Giuseppe Frisullo, Maria Laura Foddai, Iole Cordone, Paola Nisticò, et al.cells were excluded by Fixable Viability Stain 700 (BD Bioscience 564997). Cells were acquired in the BD FACSCelesta and BD FACSCantoII flow cytometers and analyzed by BD FACSDiva and Flowjo software. Statistical analysis For

(3) A mechanism of IL-34-induced resistance against cytotoxic anti-cancer therapies such as radiation by X-ray and chemotherapy by OxaliplatinOncoImmunologyDecember 31, 2023Nanumi Han, Haruka Wada, Takuto Kobayashi, Ryo Otsuka, Ken-Ichiro SeinoFactor staining Buffer Set (InvitrogenTM) was used for intracellular staining. Data were acquired using BD FACSCelesta (BD Biosciences) flow cytometer, and analyzed using FlowJo (BD Biosciences, USA) software. Following

(4) Immunologic Signatures of Peripheral Blood T Cells Reveal the Outcome of p53MVA Vaccine and Pembrolizumab Treatment in Patients with Advanced Ovarian CancerCancer Research CommunicationsDecember 20, 2023Ferdynand J. Kos, Paul Frankel, Mihaela Cristea, Melissa Eng, Raechelle Tinsley, Shannon Dempsey, Nora Ruel, Daphne Stewart, Thanh H. Dellinger, Don J. DiamondCD45RO-PE-CF594, CD197-PE, HLA-DR-AF700, CD38-APC (BD Biosciences) and analyzed by flow cytometry (BD FACSCelesta, BD Biosciences). Data acquired in FACSDiva (BD Biosciences) were analyzed in FlowJo (FlowJo LLC). Normal

(5) Corilagin enhances the anti-tumor activity of 5-FU by downregulating the expression of GRP 78Sci RepDecember 19, 2023Simin Li, Xinquan Li, Xiliang Yang, Yumeng Lei, Mingxin He, Xiaochen Xiang, Qingming Wu, Hongyun Liu, Jiadun Wang, Qiang Wangtemperature. Then, the samples were washed three times in phosphate-buffered saline (1 × PBS). Detected by BD FACSCelesta flow cytometry (Becton, Dickinson and Company, USA). The results were analyzed using FlowJo V10 software.

(6) Treatment with the PPARα agonist fenofibrate improves the efficacy of CD8+ T cell therapy for melanomaMolecular Therapy - OncolyticsDecember 19, 2023Mohadeseh Hasanpourghadi, Arezki Chekaoui, Sophia Kurian, Raj Kurupati, Robert Ambrose, Wynetta Giles-Davis, Amara Saha, Xu Xiaowei, Hildegund C.J. Ertl(BioLegend, San Diego, CA) at 4°C for 30 min followed by washing the cells once and analyzing them by BD FACSCelesta Cell Analyzer (BD Biosciences, San Diego, CA). Data were calculated by FlowJo (TreeStar, Woodburn, OR). Immunohistochemistry The

(7) Hepatitis D infection induces IFN-β-mediated NK cell activation and TRAIL-dependent cytotoxicityFront ImmunolDecember 8, 2023Christopher Groth, Jovana Maric, Irene Garcés Lázaro, Tomáš Hofman, Zhenfeng Zhang, Yi Ni, Franziska Keller, Isabelle Seufert, Maike Hofmann, Christoph Neumann-Haefelin, et al.564997) according to the manufacturer’s instructions. Samples were acquired on a BD LSRFortessa™ X-20 or BD FACSCelesta™. Analysis of samples was done with FlowJo v. 10.8.1. Gating for flow cytometry analysis was based on

(8) CDC7 inhibition induces replication stress-mediated aneuploid cells with an inflammatory phenotype sensitizing tumors to immune checkpoint blockadeNature CommunicationsNovember 18, 2023Tomoko Yamamori Morita, Jie Yu, Yukie Kashima, Ryo Kamata, Gaku Yamamoto, Tatsunori Minamide, Chiaki Mashima, Miyuki Yoshiya, Yuta Sakae, Toyohiro Yamauchi, et al.200–400 μL of staining buffer and incubated at 4 °C for 0–24 h in the dark until the analyses with BD FACSCelesta. The antibodies used are listed in the Source Data file. Immunohistochemistry Samples were formalin-fixed

(9) Tumor cell-induced macrophage senescence plays a pivotal role in tumor initiation followed by stable growth in immunocompetent conditionJournal for ImmunoTherapy of CancerNovember 13, 2023Haruka Wada, Ryo Otsuka, Wilfred T V Germeraad, Tomoki Murata, Toru Kondo, Ken-ichiro SeinoF4/80+ cells as previously described.17 Flow cytometry Flow cytometry was performed using an FC500 or BD FACSCelesta (BD Biosciences, Franklin Lakes, New Jersey, USA); data were analyzed using FlowJo software (Tree Star,

(10) Antler stem cell-derived exosomes promote regenerative wound healing via fibroblast-to-myofibroblast transition inhibitionJournal of Biological EngineeringNovember 8, 2023Guokun Zhang, Dongxu Wang, Jing Ren, Jiping Li, Qianqian Guo, Liyan Shi, Chunyi Lisequentially stained with AF488-labeled secondary antibodies and then quantitatively analyzed using BD FACSCelesta (BD Biosciences, USA). The antibodies are listed in Table S1. Rat bone marrow MSCs (bMSCs) and human

(11) Immune microenvironment dynamics of HER2 overexpressing breast cancer under dual anti-HER2 blockadeFrontiers in ImmunologyOctober 31, 2023Sofia Batalha, Catarina Monteiro Gomes, Catarina Brito#301806) anti-CD16-APC, anti-PD-L1-BV785 (Panel 2). Samples were analyzed by flow cytometry on the BD FACSCelesta™ Cell Analyzer (BD Biosciences) and data analysis was performed using FlowJo™ v10.7.2 software (BD Life

(12) A humanized orthotopic mouse model for preclinical evaluation of immunotherapy in Ewing sarcomaFrontiers in ImmunologyOctober 6, 2023Wen Luo, Hai Hoang, Yanling Liao, Jian Pan, Janet Ayello, Mitchell S. Cairodevice in irradiator chamber for housing mice during irradiation.- Class II biological safety cabinet.- BD FACSCelesta Cell Analyzer (BD Biosciences, La Jolla, CA) or any flow cytometer capable of five-color cytometry.-

(13) Polydom/SVEP1 binds to Tie1 and promotes migration of lymphatic endothelial cellsJournal of Cell BiologySeptember 4, 2023Ryoko Sato-Nishiuchi, Masamichi Doiguchi, Nanami Morooka, Kiyotoshi Sekiguchi488–conjugated goat anti-mouse IgG (H+L) antibody for 30 min at 4°C. Cells were then analyzed on a BD FACSCelesta flow cytometer (BD Biosciences) and BD FACSDiva software (BD Biosciences). Mouse IgG was used as a control. The collected

(14) Minichromosome maintenance proteins in lung adenocarcinoma: Clinical significance and therapeutic targetsFEBS Open BioSeptember 1, 2023Kengo Tanigawa, Yuya Tomioka, Shunsuke Misono, Shunichi Asai, Naoko Kikkawa, Yoko Hagihara, Takayuki Suetsugu, Hiromasa Inoue, Keiko Mizuno, Naohiko Seki(BD Biosciences, Franklin Lakes, NJ, USA) was used for the evaluation of the cell cycle distribution on a BD FACSCelesta Flow Cytometer (BD Biosciences). The data were analyzed using FlowJo software (TreeStar, Ashland, OR, USA). The procedures

(15) Targeting macrophage M1 polarization suppression through PCAF inhibition alleviates autoimmune arthritis via synergistic NF-κB and H3K9Ac blockadeJ NanobiotechnologyAugust 19, 2023Jinteng Li, Feng Ye, Xiaojun Xu, Peitao Xu, Peng Wang, Guan Zheng, Guiwen Ye, Wenhui Yu, Zepeng Su, Jiajie Lin, et al.cells were resuspended in 200 µl of PBS and collected into tubes. All samples were analyzed using a BD FACSCelesta flow cytometer. Immunoprecipitation assays The cells were quickly harvested and homogenized on ice in lysis buffer

(16) Transcriptional and chromatin profiling of human blood innate lymphoid cell subsets sheds light on HIV‐1 pathogenesisThe EMBO JournalAugust 15, 2023Yetao Wang, Lawrence Lifshitz, Noah J Silverstein, Esther Mintzer, Kevin Luk, Pamela StLouis, Michael A Brehm, Scot A Wolfe, Steven G Deeks, Jeremy Luban(2019),http://satijalab.org/seurat Other,Other,Other Illumina NexSeq 500,Illumina, BD FACSAria IIu,BD, BD FACSCelesta,BD, 4D‐Nucleofector,Lonza, 4200 TapeStation,Agilent,

(17) Targeting of SLC25A22 boosts the immunotherapeutic response in KRAS-mutant colorectal cancerNature communicationsAugust 4, 2023Zhou Q, Peng Y, Ji F, Chen H, Kang W, Chan LS, Gou H, Lin Y, Huang P, Chen D, et al.eBioscience) and stained with antibodies against intracellular markers. Flow cytometry was performed on BD FACSCelesta (BD Biosciences), and data were analyzed by FlowJo software (FlowJo v10). Tumor models and treatments Male

(18) The Identification of CELSR3 and Other Potential Cell Surface Targets in Neuroendocrine Prostate CancerCancer Res CommunAugust 1, 2023Van Emmenis L, Ku SY, Gayvert K, Branch JR, Brady NJ, Basu S, Russell M, Cyrta J, Vosoughi A, Sailer V, et al.Labs #815) were similarly stained with the proprietary CELSR3 flow antibody. Samples were run on a BD FACSCelesta flow cytometer (BD Biosciences) and mean fluorescence intensities recorded for at least 10,000 events per sample. A

(19) Therapeutic Effects of Gingival Mesenchymal Stem Cells and Their Exosomes in a Chimeric Model of Rheumatoid ArthritisResearch SquareJuly 6, 2023Shane Bruckner, Vittoria M Capria, Braden Zeno, Binnaz Leblebicioglu, Kanu Goyal, William K Vasileff, Hisham Awan, William L Willis, Latha P Ganesan, Wael N Jarjourisolated and digested in collagenase to release adherent cells. The cell suspension was analyzed on a BD FACSCelesta ow cytometer (BD, Franklin Lakes, NJ) and data were analyzed by using FlowJo v10.8.1 (Ashland, OR). The

(20) Skullcapflavone II, a novel NQO1 inhibitor, alleviates aristolochic acid I-induced liver and kidney injury in miceActa Pharmacologica SinicaJuly 1, 2023Ya-ping Dong, Shu-zhen Chen, Hui-si He, Zhuo-ran Sun, Li-xuan Jiang, Yan-qiu Gu, Ying Zhang, Fei Feng, Chun Chen, Zhe-cai Fan, et al.sample tube. Cells and reagents were incubated at room temperature in the dark for 20 min, and then BD FACSCelesta (BD, Franklin Lakes, NJ, USA) was used for flow cytometry analysis. The quantitative real-time PCR

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(1)

12/28/2018Awesome Flow Cytometry Analyzer (12 Colors)Dr. Min Hee OhAs an immunologist, we heavily rely on flow cytometry for most experiments. Having the BD Celesta in our lab has greatly facilitated our research to perform multi-color flow cytometry. Routine ... Read Review
(1)

12/10/2018Great Cytometer Hong SunWe are interested in studying the mechanism of T cell differentiation through modulating the mTOR pathway. This flow cytometer is easy to set up and maintain and is sufficient for distinguishing ... Read Review
(1)

7/27/2018Reliable Flow Cytometer and Worth the Money Meng SunOur lab starting using this machine as our go-to procedure for identifying cells in specific tumor cells ex-vivo. This machine has variety of colors for each antibody so we were able to identify a ... Read Review
(1)

2/26/2018Great Flow Cytometry Analyzer For Up To 14 Parameters (12 Colors) Chirag PatelWe are an immunology lab that studies the adaptive and innate system. We primarily are a flow based lab since most of our experiments rely on analyzing different cell populations in the context to ... Read Review

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BD FACSCelesta™ Flow Cytometer from BD Biosciences

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BD FACSCelesta™ Flow Cytometer from BD Biosciences

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