NGC and ChromLab 6.1 from Bio-Rad

Product Specs
ItemNGC and ChromLab 6.1
CompanyBio-Rad
Price Get Pricing Get Quote for this Product
Catalog Number7880003 / 7880007 / 7880009 / 7880011
Dimensions(WxDxH) 61 x 49 x 56 cm (24 x 19 x 22 inches)
QuantityEA
ApplicationsBiomolecule Purification
DetectionSingle- Wavelength (UV)/ Multi - Wavelength (UV/Vis)
Flow Rate0.001–10 mL/minute / 0.01 -10 mL/minute
Brand/TrademarkBio-Rad Laboratories, Life Science Group
Product TypeChromatography System

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) An Exploration of Small Molecules That Bind Human Single-Stranded DNA Binding Protein 1Biology (Basel)November 6, 2023Zachariah P. Schuurs, Alexander P. Martyn, Carl P. Soltau, Sam Beard, Esha T. Shah, Mark N. Adams, Laura V. Croft, Kenneth J. O’Byrne, Derek J. Richard, Neha S. Gandhisupernatant was resolved on a 20 mL SP Sepharose Fast Flow column (GE Healthcare, Chicago, IL, USA) using an NGC FPLC (BioRad, Hercules, CA, USA) with a 5-column volume gradient of 100 to 1000 mM KCl in buffer K (20

(2) Comparative analysis of the physical properties of murine and human S100A7: Insight into why zinc piracy is mediated by human but not murine S100A7Journal of Biological ChemistryNovember 1, 2023Simone A. Harrison, Anais Naretto, Swati Balakrishnan, Yasiru R. Perera, Walter J. Chazin(Cytiva) previously equilibrated with the Buffer A (20 mM sodium acetate at pH 5.4) using a BioRad NGC FPLC system at 4 °C. The protein was eluted using a linear gradient over 10 column volumes generated

(3) A marine cryptochrome with an inverse photo-oligomerization mechanismNature CommunicationsOctober 30, 2023Hong Ha Vu, Heide Behrmann, Maja Hanić, Gayathri Jeyasankar, Shruthi Krishnan, Dennis Dannecker, Constantin Hammer, Monika Gunkel, Ilia A. Solov’yov, Eva Wolf, et al.light conditions using a Superdex 200 10/300 GL column wrapped in aluminum foil connected to a Biorad NGC Quest10 Plus Chromatography System. For each SEC run, 100 µL sample diluted to a concentration of 2

(4) The Generation of a Nanobody-Based ELISA for Human Microsomal Epoxide HydrolaseInternational Journal of Molecular SciencesSeptember 28, 2023Qiyi He, Mark R. McCoy, Meng Qi, Christophe Morisseau, Huiyi Yang, Chengpeng Xu, Rachel Shey, Michael C. Goodman, Suqing Zhao, Bruce D. Hammockpurified by size exclusion chromatography. Briefly, 1 mL of purified protein was injected into a Bio-Rad NGC FPLC system equipped with a Bio-Rad Enrich High-Resolution Size Exclusion column. Protein was eluted

(5) Structural basis for the selective methylation of 5-carboxymethoxyuridine in tRNA modificationNucleic Acids ResearchSeptember 22, 2023Jaehun Yoo, Jangmin Lee, Jungwook KimCmoM was determined by size exclusion using Superdex 75gl column (GE Healthcare, Life Sciences) on NGC FPLC system (Biorad). 100 μg of the wild-type CmoM (calculated monomer molecular weight = 31.7 kDa)

(6) Protocol to identify amino acids bound to tRNA by aminoacylation using mass spectrometrySTAR ProtocAugust 15, 2023Marc Finkler, Joshua Brandt, Timothée Boutfol, Florent Grimm, Philip Hartz, Albrecht Ottplastic spring, 0.1 mL (100 μL inserts)",VWR,MANA702818 NGC Quest 10 Plus Chromatography System,Bio-Rad,7880003 "Pierce Concentrator, PES, 3k MWCO, 0.5 mL",Thermo Fisher Scientific,88512 Prominence UFLC XR System,Shimadzu,Controller:

(7) Development of Chimeric Hepatitis B (HBV) – Norovirus (NoV) P particle as candidate vaccine against Hepatitis B and norovirus infectionVaccine: XAugust 1, 2023Ernawati Arifin Giri-Rachman, Marselina Irasonia Tan, Ashwin Ramesh, Putri Ayu Fajar, Annisa Nurul Ilmi, Debbie Sofie Retnoningrum, Rukman Hertadi, Apriliani Irawan, Gladys Emmanuella Putri Wojciechowska, Lijuan Yuansolubilized protein was performed in prepacked cOmplete™ His-Tag Purification Column (Roche) using NGC Medium-Pressure Liquid Chromatography Systems (Bio-Rad). Fractions were washed using wash buffer (pH

(8) Self-assembling Gn head ferritin nanoparticle vaccine provides full protection from lethal challenge of Dabie Bandavirus in aged ferretsbioRxivJuly 24, 2023Dokyun Kim, Eunha Kim, Semi Kim, Youseung Chung, Chih-Jen Lai, Inho Cha, Sung-Dong Cho, Yunseo Choi, Xinghong Dai, Stephanie Kim, et al.supernatants were flowed into anion Resource Q column (Cytiva) for anion exchange chromatography on NGC FPLC (Bio-Rad) running with pH8.0, 20mM Tris-Cl and a gradient increase from 0M to 1M NaCl at 3.0ml/min.

(9) Transcriptomic Analysis Followed by the Isolation of Extracellular Bacteriolytic Proteases from Lysobacter capsici VKM B-2533TInternational Journal of Molecular SciencesJuly 19, 2023Alexey Afoshin, Irina Kudryakova, Sergey Tarlachkov, Elena Leontyevskaya, Dmitry Zelenov, Pavel Rudenko, Natalya Leontyevskaya (Vasilyeva)Furthermore, an individual purification scheme was developed for each bacteriolytic enzyme using the NGC chromatographic system (Bio-Rad, Hercules, CA, USA). At the first stage, the protein precipitate after

(10) In-Depth Characterization of Apoptosis N-terminome Reveals a Link Between Caspase-3 Cleavage and Post-Translational N-terminal AcetylationMolecular & Cellular ProteomicsJuly 1, 2023Rawad Hanna, Andrey Rozenberg, Layla Saied, Daniel Ben-Yosef, Tali Lavy, Oded Kleifeldmin), and the clear lysate was purified on HisTrap HP 1 ml affinity column (Cytiva) by FPLC system (NGC, Bio-Rad). The protein was eluted from the column using a linear gradient (elution buffer: 0–1 M imidazole,

(11) Catalytic Cycle of the Bifunctional Enzyme Phosphoribosyl-ATP Pyrophosphohydrolase/Phosphoribosyl-AMP CyclohydrolaseACS CatalysisJune 2, 2023Gemma Fisher, Ennio Pečaver, Benjamin J. Read, Susannah K. Leese, Erin Laing, Alison L. Dickson, Clarissa M. Czekster, Rafael G. da Silvawas loaded onto a 20 mL HiTrap Q HP column (GE Healthcare) pre-equilibrated with water in a Bio-Rad NGC FPLC. The column was washed with 3 CV of water and 5 CV of either 6% or 18% solution B (1 M ammonium

(12) The T1150A cancer mutant of the protein lysine dimethyltransferase NSD2 can introduce H3K36 trimethylationJournal of Biological ChemistryJune 1, 2023Mina S. Khella, Philipp Schnee, Sara Weirich, Tan Bui, Alexander Bröhm, Pavel Bashtrykov, Jürgen Pleiss, Albert JeltschGF/PET 45, MachereyNagel) and passed through a HiTrap SP HP (5 ml, GE Healthcare) column connected to an NGC FPLC system (BioRad), which was previously equilibrated with SAU buffer. After washing the column with

(13) The TUDOR domain of SMN is an H3K79me1 histone mark readerLife Science AllianceJune 1, 2023Olivier Binda, Aimé Boris Kimenyi Ishimwe, Maxime Galloy, Karine Jacquet, Armelle Corpet, Amélie Fradet-Turcotte, Jocelyn Côté, Patrick Lomonteon a 5-ml HiTrap SP FF (GE Healthcare) cation exchange column on a next-generation chromatography (NGC, Bio-Rad). Fractions containing the purified histone were pooled and dialyzed three times into 4 liters

(14) Polypyrimidine tract binding protein 1 (PTBP1) contains a novel regulatory sequence, the rBH3, that binds the pro-survival protein MCL1Journal of Biological ChemistryJune 1, 2023Christine Carico, Jia Cui, Alexus Acton, William J. Placzekfiltered through a 0.45 μm syringe filter (Millex). Recombinant protein is then purified on a Bio-Rad NGC FPLC system using nickel chromatography (1 ml HisTrap; GE Healthcare) followed by gel filtration on

(15) High performance anion exchange chromatography purification of probiotic bacterial extracellular vesicles enhances purity and anti-inflammatory efficacybioRxivMay 5, 2023Nicholas H. Piroli, Laura Samantha C. Reus, Zuzanna Mamczarz, Sulayman Khan, William E. Bentley, Steven M. Jaycolumns. High performance AEC was performed using a Bio-Rad NGC Quest 10 Plus Chromatography System (Bio-Rad; 7880003) equipped with a 5 mL HiTrap Q column (Cytiva; 17115401) that contains a strong anion exchange resin

(16) From methodological limitations to the function of metallothioneins - a guide to approaches for determining weak, moderate, and tight affinity zinc sitesMetallomicsMay 2, 2023Adam Pomorski, Agnieszka Drozd, Anna Kocyła, Artur KrężelENrich SEC-70 gel filtration column (Bio-Rad) equilibrated with 20 mM Tris–HCl buffer, pH 8.6 (Bio-Rad NGC).27 In the second approach, Zn(II)-loaded MT2 was obtained in such a way as to avoid protein acidification.

(17) Comparing the Effect of HPP on the Structure and Stability of Soluble and Membrane-Bound Polyphenol Oxidase from ‘Lijiang Snow’ Peach: Multispectroscopic and Molecular Dynamics SimulationFoodsApril 27, 2023Hengle Zhou, Shenke Bie, Zi Li, Linyan Zhouwas purified by DEAE-Sepharose fast flow column chromatography using a chromatography system (Bio-Rad NGC, Hercules, CA, USA), and mPPO was purified by DEAE-Sepharose fast flow and Sephacryl S-200 HR 16/60

(18) Dephosphocholination by Legionella effector Lem3 functions through remodelling of the switch II region of Rab1bNature CommunicationsApril 19, 2023Marietta S. Kaspers, Vivian Pogenberg, Christian Pett, Stefan Ernst, Felix Ecker, Philipp Ochtrop, Michael Groll, Christian Hedberg, Aymelt Itzencleared by centrifugation. All chromatography steps during protein purification were performed using NGC medium-pressure liquid chromatography (LC) system (Bio-Rad Laboratories). Cleared lysates were supplemented

(19) Crystal structure of a highly conserved enteroviral 5′ cloverleaf RNA replication elementNature CommunicationsApril 7, 2023Naba K. Das, Nele M. Hollmann, Jeff Vogt, Spiridon E. Sevdalis, Hasan A. Banna, Manju Ojha, Deepak Koiralalysate was filtered through a 0.45-micron filter (VWR), and the Fab was purified using the Bio-Rad NGC fast protein liquid chromatography (FPLC) system. First, the lysate was passed through a Hi-trap protein A

(20) Fortuitously compatible protein surfaces primed allosteric control in cyanobacterial photoprotectionNature Ecology & EvolutionApril 3, 2023Niklas Steube, Marcus Moldenhauer, Paul Weiland, Dominik Saman, Alexandra Kilb, Adán A. Ramírez Rojas, Sriram G. Garg, Daniel Schindler, Peter L. Graumann, Justin L. P. Benesch, et al.100% of buffer B (300 mM NaCl, 20 mM Tris, 500 mM imidazole, 5 mM β-mercaptoethanol, pH 8.0) in an NGC system (BioRad). Fractions containing the protein were verified on in-house casted 15% SDS gels, and

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