Wide Mini-Sub Cell GT System from Bio-Rad

Product Specs
ItemWide Mini-Sub Cell GT System
CompanyBio-Rad
Price Pricing Info Supplier Page
Catalog Number1704468
Quantity1EA
Brand/TrademarkBio-Rad Laboratories, Life Science Group
Number of SamplesUp to 60 samples (15- and 20-wells)
Internal Dimensions15 x 10 cm gel tray

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Whole Transcriptome Analysis of Intervention Effect of Sophora subprostrate Polysaccharide on Inflammation in PCV2 Infected Murine Splenic LymphocytesCurrent Issues in Molecular BiologyJuly 20, 2023Yi Zhao, Nina Jia, Xiaodong Xie, Qi Chen, Tingjun HuSubsequently, the protein was transferred onto the PVDF membrane. The Power Pac basic power supply, Wide mini-sub cell GT system, Trans-Blot SD Semi-Dry electrophoretic transfer tank were all provided by Bio-Rad (Bio-Rad Laboratories,

(2) Reconfigurable pH-Responsive DNA Origami LatticesACS NanoJune 13, 2023Sofia Julin, Veikko Linko, Mauri A. Kostiainensamples in the gel pockets. The gel was run for 45 min at a constant voltage of 95 V using a BioRad Wide Mini-Sub Cell GT System and a BioRad PowerPac Basic power supply while keeping the gel electrophoresis chamber on an ice bath.

(3) Identification of a Novel Curcumin Derivative Influencing Notch Pathway and DNA Damage as a Potential Therapeutic Agent in T-ALLCancers (Basel)November 24, 2022Nadezda Zhdanovskaya, Sara Lazzari, Diego Caprioglio, Mariarosaria Firrincieli, Chiara Maioli, Eleonora Pace, Daniela Imperio, Claudio Talora, Diana Bellavia, Saula Checquolo, et al.the dark to allow DNA unwinding. Slides were then placed on a horizontal gel electrophoresis chamber (1704468; Biorad) filled with cold alkaline electrophoresis solution (300 mM NaOH (S8045; Sigma-Aldrich) and

(4) TOP1 CAD-seq: A protocol to map catalytically engaged topoisomerase 1 in human cellsSTAR ProtocolsSeptember 16, 2022Vladislav Kuzin, Anika Wiegard, Donald P. Cameron, Laura Baranelloamount of loaded sample if you have lower volume of wells.37.Run the gel at 200 V for 20 min in Bio-Rad Wide Mini-Sub Cell GT System (adjust the time and voltage for other gel chambers accordingly) or until the bromophenol blue reaches

(5) Protocol to study sufficiency of cis-regulatory elements in mouse embryonic stem cells using a CRISPR-mediated knockin approachSTAR ProtocolsSeptember 16, 2022Tomas Pachano, Alvaro Rada-Iglesiasthermal cycler,Bio-Rad,Cat: 1851148 Wide Mini-Sub Cell GT Horizontal Electrophoresis System,Bio-Rad,Cat: 1704468 ZOE™ Fluorescent Cell Imager,Bio-Rad,Cat: 1450031 TC20 Automated Cell Counter,Bio-Rad,Cat: 1450102

(6) The 4G/5G Polymorphism of Plasminogen Activator Inhibitor Type 1 is a Predictor of Moderate-to-Severe Thyroid Eye DiseaseJournal of Inflammation ResearchMay 12, 2021Monika Katko, Erika Galgoczi, Annamaria Erdei, Annamaria Gazdag, Eszter Berta, Miklos Bodor, Ildiko Seres, Ildiko Hircsu, Arpad Badics, Bernadett Ujhelyi, et al.4°C. PCR products were separated by gel electrophoresis (2% agarose gel, 100 V, 50 minutes) using a Wide Mini-Sub Cell GT System (Bio-Rad Laboratories, Hercules, CA, USA), stained with GelRed (Biotium Inc, Fremont, CA, USA) and visualized

(7) Expression Landscape of circRNAs in Arabidopsis thaliana Seedlings and Adult TissuesFrontiers in Plant ScienceSeptember 10, 2020Anna Philips, Katarzyna Nowis, Michal Stelmaszczuk, Paulina Jackowiak, Jan PodkowiÅ„ski, Luiza Handschuh, Marek Figlerowiczusing the QIAquick PCR Purification Kit (Qiagen), resolved in 2% agarose gel for 70 min at 120 V in a Wide Mini-Sub Cell GT System (Bio-Rad) and poststained with 0.5 µg/ml EtBr solution for 20 min on a rocker. Gel images were obtained

(8) Arabidopsis thaliana cbp80, c2h2, and flk Knockout Mutants Accumulate Increased Amounts of Circular RNAsCellsAugust 21, 2020Anna Philips, Katarzyna Nowis, Michal Stelmaszczuk, Jan PodkowiÅ„ski, Luiza Handschuh, Paulina Jackowiak, Marek Figlerowiczpurification of PCR products, which were then resolved on a 2% agarose gel for 70 min at 120 V in a Wide Mini-Sub Cell GT System (Bio-Rad, Hercules, CA, USA), and subsequently stained with a 0.5 µg/mL EtBr solution for 20 min on

(9) A Primase-Induced Conformational Switch Controls the Stability of the Bacterial ReplisomeMolecular CellJuly 1, 2020Enrico Monachino, Slobodan Jergic, Jacob S. Lewis, Zhi-Qiang Xu, Allen T.Y. Lo, Valerie L. O’Shea, James M. Berger, Nicholas E. Dixon, Antoine M. van OijenGeneRuler DNA Ladder mixes were loaded as marker. Gels were run in 2x TAE buffer for 150 min at 60 V in a Wide Mini-Sub Cell GT System (Bio-Rad), followed by staining with 1x SYBR Gold in 2x TAE buffer for 2 h (Figure 5C). Quantification

(10) A simple genotyping method to detect small CRISPR-Cas9 induced indels by agarose gel electrophoresisScientific ReportsDecember 1, 2019Debanjan Bhattacharya, Erwin G. Van Meirquickly. The gel tray was placed on a wide mini-sub cell GT horizontal electrophoresis system (Bio-Rad, 1704468) and the electrophoresis chamber filled with 1X TAE buffer till about 1 cm above the gel. 10 µl of each

(11) Glomerular Collagen Deposition and Lipocalin-2 Expression Are Early Signs of Renal Injury in Prediabetic Obese RatsInternational journal of molecular sciencesAugust 30, 2019Bukosza EN, Kaucsár T, Godó M, Lajtár E, Tod P, Koncsos G, Varga ZV, Baranyai T, Nguyen MT, Schachner H, et al.integrity, the samples were electrophoresed on 1% agarose gel (Invitrogen Ltd., Paisley, UK) in BioRad Wide mini-sub® cell GT system (Bio-Rad Laboratories, Inc., Hercules, CA, USA), and the ratio of 28S ribosomal RNA bands was calculated.

(12) Investigation of the Antioxidant, Î±-Glucosidase Inhibitory, Anti-inflammatory, and DNA Protective Properties of Vaccinium arctostaphylos LTurkish Journal of Pharmaceutical SciencesJune 1, 2019Burak BARUT, Elif Nur BARUT, SeÃ§kin ENGÄ°N, Arzu Ã–ZEL, Feride Sena SEZENmixtures were loaded on agarose gel and electrophoresis was performed at 100 V for 90 min using the wide Mini-Sub cell GT system from Bio-Rad. The results were visualized with the Bio-Rad Gel Doc XR system.32 In vivo anti-inflammatory

(13) In vitro Generation of CRISPR-Cas9 Complexes with Covalently Bound Repair Templates for Genome Editing in Mammalian CellsBIO-PROTOCOLJanuary 5, 2019Nataša Savić, Femke Cas Ringnalda, Christian Berk, Katja Bargsten, Jonathan Hall, Martin Jinek, Gerald SchwankWide Mini-Sub® Cell GT horizontal electrophoresis system, 15 x 10 cm tray (BioRad, catalog number: 1704468) ChemiDoc XRS gel imaging system (Bio-Rad, catalog number: 1708265) Liquid chromatography-mass spectrometry

(14) The interactions of novel mononuclear platinum-based complexes with DNABMC CancerDecember 22, 2018Ben W. Johnson, Mark W. Burgess, Vincent Murray, Janice R. Aldrich-Wright, Mark D. Templeratio, made up to a final volume of 6.25 μL. The 1% (w/v) agarose gel were cast and run on a Bio-Rad Wide Mini-Sub Cell GT System with 6.25 μL drug-treated plasmid DNA samples or untreated plasmid DNA sample (DMF solvent control)

(15) Design of DNA rolling-circle templates with controlled fork topology to study mechanisms of DNA replicationAnalytical BiochemistrySeptember 1, 2018Enrico Monachino, Harshad Ghodke, Richard R. Spinks, Ben S. Hoatson, Slobodan Jergic, Zhi-Qiang Xu, Nicholas E. Dixon, Antoine M. van Oijencast with 0.8 g/mL ethidium bromide. Electrophoresis in 1x TAE buffer was at 82 V for 85 min in a Wide Mini-Sub Cell GT System (Bio-Rad). DNA was visualized using a Bio-Rad Gel Doc XR (302 nm trans-UV light). Cy5-labeled DNA gels: Cy5-labeled

(16) Design and assessment of engineered CRISPR–Cpf1 and its use for genome editingNature ProtocolsMay 1, 2018Bin Li, Chunxi Zeng, Yizhou Dongpurification system (Millipore) Wide Mini-Sub cell GT horizontal electrophoresis system (Bio-Rad, cat. no. 1704468) REAGENT SETUP Complete growth medium Add 50 ml of FBS to 450 ml of DMEM. Filter the mixed solution

(17) Design of Hybrid RNA Polymerase III Promoters for Efficient CRISPR-Cas9 FunctionBIO-PROTOCOLMarch 20, 2018Joshua Misa, Cory Schwartz, Ian Wheeldon51028063) 6. Gel electrophoresis tank (Bio-Rad Laboratories, model: Wide Mini-Sub®, catalog number: 1704468) 7. Gel electrophoresis power supply (Bio-Rad Laboratories, model: PowerPacTM Basic, catalog number:

(18) Assessment of Potato Resistance to Synchytrium EndobioticumPlant Breeding and Seed ScienceDecember 20, 2017Jarosław Przetakiewicz, Jarosław Plichepgradient) 2) Equipment for agarose gel electrophoresis (BioRad, PowerPac Basic, cat. No 1645050) 3) Wide Mini-Sub Cell GT System, cat. No 170-4468) 4) UV lighter for visualization of obtained products PROCEDURE Phenotypical assessment A

(19) Interfacial analysis of siRNA complexes with poly-ethylenimine (PEI) or PAMAM dendrimers in gene deliveryColloids and Surfaces B: BiointerfacesOctober 1, 2017Patrick K.C. Chang, Clive A. Prestidge, Kristen E. Bremmell(TAE) buffer (Tris-base, glacial acetic acid, EDTA, Milli-Q) containing GelRad dye using a Bio-Rad Wide Mini Sub-Cell GT system at 80 V for 40 min. Digital imaging was performed on a Bio-Rad ChemiDoc system using Quantity One v4.6.3

(20) Synthesis, in vitro DNA interactions, cytotoxicities, antioxidative activities, and topoisomerase inhibition potentials of Mn(II), Co(II), Ni(II), Cu(II), and Zn(II) complexes with azo-oxime ligandsTURKISH JOURNAL OF CHEMISTRYJanuary 1, 2017Melek ÇOL AYVAZ, İbrahim TURAN, Bayram DURAL, Selim DEMİR, Kaan KARAOĞLU, Yüksel ALİYAZICIOĞLU, Kerim SERBESTUV-Vis spectrophotometer from 200 to 600 nm. Cleavage experiments were performed with the help of the Wide Mini-Sub Cell GT System (Bio-Rad) supported by PowerPacBasic (BioRad), and gel patterns of electrophoresis were visualized under

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12/6/2016Great System For Running Gels Rebecca GuyerWe have various genetically modified mouse lines which require genotyping via PCR. After PCR we need to run the amplified product on an agarose gel before staining. This system makes the perfect ... Read Review