Siloam Biosciences’ Optimiser technology offers a rapid and sensitive chemifluorescent-based ELISA procedure that uses very small sample volumes. The speed, sensitivity, and small sample requirements are enabled by the unique microfluidic design of the Optimiser microplate. Standard immunoassay reactions such as analyte capture and detection occur within a ~ 5 µL microfluidic reaction chamber. The unique microchannel geometry and small reaction volumes favor rapid reaction kinetics. The typical assay procedure utilizes a 5 µL sample and each reaction step is completed in 10 - 20 minutes. With wash time, substrate incubation time, and read time accounted for, a typical assay can be completed within approximately 2 hours.