Crab Duplex-Specific Thermostable Nuclease from Evrogen JSC

• Selectively cleaves ds DNA and DNA in DNA-RNA hybrid duplexes
• Discriminates between perfectly and nonperfectly matched short duplexes
• Inactive towards ssDNA and RNA
• Thermostable
• Inhibited by EDTA

Duplex-specific nuclease (DSN) is an enzyme purified from hepatopancreas of Red King (Kamchatka) crab. DSN shows a strong preference for cleaving double-stranded (ds) DNA and DNA in DNA-RNA hybrid duplexes, and is practically inactive towards single-stranded (ss) DNA or single or double-stranded RNA. Moreover, the enzyme is capable of discriminating between perfectly and imperfectly matched short DNA duplexes.

DSN –based protocols developed by Evrogen and independent teams are proved to be a convenient and effective approach to remove repeated sequences from DNA mixture. The list of approved DSN applications includes full-length enriched cDNA normalization, construction of normalized RNA-seq libraries for next generation sequencing, cDNA depletion (e.g., specific removal of non-mRNA-derived transcripts from cDNA), etc. The protocols exploit nucleic acid hybridization kinetics and unique specificity of DSN to the double-stranded DNA, and are now compatible with all main NGS platforms.