Rapid DNA Dephos & Ligation Kit; Convenient kit for rapid and efficient DNA dephosphorylation and ligation from Roche Applied Science

Rapid DNA Dephos & Ligation Kit; Convenient kit for rapid and efficient DNA dephosphorylation and ligation from Roche Applied Science

Description

The Rapid DNA Dephos & Ligation Kit enables fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments. Ligation is performed in just 5 minutes at +15 to +25°C; dephosphorylation of blunt and sticky 5'-overhang en

Specifications

Specifications/Features

Information:
DNA ligases join linear DNA fragments together via covalent bonds. DNA ligation involves creating a phosphodiester bond between the 3' hydroxyl group of one nucleotide and the 5' phosphate of another. Ligation of DNA fragments is an essential step in many molecular biology techniques, including gene cloning and messenger RNA (mRNA) fingerprinting. For efficient ligation, DNA strands must be prevented from self-ligating (self-circularization and concatenation) by dephosphorylation of DNA ends. The enzyme used to ligate DNA fragments is T4 DNA ligase, originally isolated from the T4 bacteriophage. This enzyme will ligate DNA fragments that have blunt ends or overhanging, cohesive ends that are annealed together.

Roche Applied Science
Roche Applied Science
Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road
Indianapolis, Indiana
United States
Phone: 800-262-1640
800-262-4911
800-428-2883

Citations