TNT^® SP6 Quick Coupled Transcription/Translation System, Trial Size from Promega

The TNT® Quick Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic in vitro protein expression. The original TNT® Coupled Reticulocyte Lysate Systems simplified the process and reduced the time required to obtain in vitro translation results compared with standard rabbit reticulocyte lysate systems. Standard rabbit reticulocyte systems commonly use RNA synthesized in vitro from SP6, T3 or T7 RNA polymerase. The TNT® Quick Coupled Transcription/Translation System further simplifies the process by combining the RNA Polymerase, nucleotides, salts, amino acids and Recombinant RNasin® Ribonuclease Inhibitor with the reticulocyte lysate solution to form a single TNT® Quick Master Mix. For most gene constructs, the TNT® Quick reaction produces significantly more protein (two- to sixfold) in a 60- to 90-minute reaction than do standard in vitro rabbit reticulocyte lysate reactions using RNA templates. Magnesium Acetate, 25mM, and Potassium Chloride, 2.5M, can be used to optimize in vitro translation reactions in the TNT® T7 Quick Coupled Transcription/Translation System and Flexi® Rabbit Reticulocyte Lysate System.

Features:
Reliable: The TNT® Systems are rigorously quality controlled to ensure the highest level of transcription/translation, whether your template is a linear (T7 only) or circular plasmid. • Quick: One-tube reaction. • Complete: Reagents including Recombinant RNasin® Ribonuclease Inhibitor and amino acids are included in the Quick Master Mix. • Good Value: One-tube format means no leftover reagents. • High Yield: Produces two- to sixfold more protein than standard in vitro rabbit reticulocyte lysates. • Flexible: Optimized for transcription/translation with selected RNA polymerase. Flexibility to optimize magnesium and potassium levels for optimal translation of a specific gene. • Versatile: The T7 system will produce protein from both linear and circular DNA (use only circular DNA with the SP6 system; for PCR(f) templates use Cat.# L5540, TNT® T7 Quick for PCR DNA). • Controls Included: Luciferase Control DNA and Luciferase Assay Reagent are included with the system as functional controls. Only full-length luciferase is active.

Applications
Rapid screening and analysis of mutants. • Rapid confirmation of gene products. • Protein:protein interactions (GST pulldown). • Protein:DNA interactions. • Protein Truncation Test (PTT). • Simultaneous expression of multiple gene products. • Protein activity studies. • Screening of chemical libraries for effects on translation. • Antibody evolution using ribosome display. • In vitro expression cloning.