Profinity IMAC Resins from Bio-Rad

Product Specs
ItemProfinity IMAC Resins
CompanyBio-Rad
Price Supplier Page View Company Product Page
Catalog Number1560121 / 1560123 / 1560125 / 1560127 / 1560131 / 1560133 / 1560135 / 1560137
QuantityEA
ModeAffinity Purification, Metal Chelate
TypeResins

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) A Multipronged Unbiased Strategy Guides the Development of an Anti-EGFR/EPHA2–Bispecific Antibody for Combination Cancer TherapyClinical Cancer ResearchJuly 14, 2023Amr El Zawily, Frederick S. Vizeacoumar, Renuka Dahiya, Sara L. Banerjee, Kalpana K. Bhanumathy, Hussain Elhasasna, Glinton Hanover, Jessica C. Sharpe, Malkon G. Sanchez, Paul Greidanus, et al.detection. Fab fragments were purified using Ni+-resin (Profinity IMAC Resin, Ni-charged, Bio-Rad, 1560135). IgGs were expressed in CHO-S culture grown in Dynamis medium (Thermo Fisher Scientific, A2661501)

(2) Functional membrane microdomains and the hydroxamate siderophore transporter ATPase FhuC govern Isd-dependent heme acquisition in Staphylococcus aureuseLifeApril 12, 2023Lea Antje Adolf, Angelika Müller-Jochim, Lara Kricks, Jan-Samuel Puls, Daniel Lopez, Fabian Grein, Simon Heilbronnercompound, drug",DDM,Roth,CN26.1, "Chemical compound, drug",Profinity IMAC Resin Ni-charged,Bio-Rad,1560135, Other,Ferrichrome,EMC Microcollections,FCH,See Materials and methods: Growth in iron-limited medium Other,Aerobactin,EMC

(3) Biochemical and Kinetic Characterization of the Glucose-6-Phosphate Dehydrogenase from Helicobacter pylori Strain 29CaPMicroorganismsJuly 6, 2022Paulina Ortiz-Ramírez et al.(Madison, WI, USA). The GeneJET Gel Extraction Kit was obtained from Thermo Scientific (Hudson, NH, USA). Profinity IMAC Resins and Gel Filtration Standard, used as a lyophilized mixture of molecular-weight markers, ranging from

(4) Overexpression of HLH4 Inhibits Cell Elongation and Anthocyanin Biosynthesis in Arabidopsis thalianaCellsMarch 24, 2022Quancan Hou et al.plasmid was transformed into Escherichia coli BL21 (DE3) cells and purified using Ni-charged resin (Cat#1560131, BioRad, Hercules, CA, USA) following the manufacturer’s instructions. The purified protein was desalted

(5) The Hippo pathway kinases LATS1 and LATS2 attenuate cellular responses to heavy metals through phosphorylating MTF1Nature cell biologyJanuary 1, 2022Han H, Nakaoka HJ, Hofmann L, Zhou JJ, Yu C, Zeng L, Nan J, Seo G, Vargas RE, Yang B, et al.were counterstained with Mayer hematoxylin. Metal binding assay Metal beads were generated using Profinity™ IMAC resins (Bio-Rad) according to the manufacturer’s protocol. Briefly, IMAC resins were washed with ultra-pure

(6) Piperidine-4-Carboxamides Target DNA Gyrase in Mycobacterium abscessusAntimicrobial Agents and ChemotherapyJuly 16, 2021Dereje Abate Negatu et al.(8,000 rpm, 4°C), and the clarified lysate was passed over an Ni gravity column (IMAC nickel resin, 1560131; Bio-Rad, USA) preequilibrated with the buffer A500. The column was washed with 10 column volumes of

(7) Autism-associated SHANK3 missense point mutations impact conformational fluctuations and protein turnover at synapseseLifeMay 4, 2021Bucher M, Niebling S, Han Y, Molodenskiy D, Hassani Nia F, Kreienkamp HJ, Svergun D, Kim E, Kostyukova AS, Kreutz MR, et al.protein purification methods "Chemical compound, drug","Profinity IMAC resin, Ni2+-charged",Bio-Rad,1560135,for protein purification "Chemical compound, drug",RFP-trap agarose,Chromotek,rta-20,for co-IP "Chemical

(8) Effect of Posttranslational Modifications on the Structure and Activity of FTO DemethylaseInternational Journal of Molecular SciencesApril 26, 2021Michał Marcinkowski et al.800 µL of Ni-charged resin suspension (Profinity™ IMAC Ni-Charged Resin, BIO-RAD, Hercules, CA, USA, #1560133) was performed at 4 ◦ C. The unbound fraction was removed by washing three times with lysis buffer.

(9) The antagonistic MYB paralogs RH1 and RH2 govern anthocyanin leaf markings in Medicago truncatulaNew PhytologistMarch 1, 2021Chongnan Wang et al.His‐TF‐RH1, His‐TF‐RH2 and His‐TF (control) were purified using Profinity IMAC Ni‐charged Resin (no. 1560131; Bio‐Rad) according to the manufacturer’s protocol and quantified by the Bio‐Rad protein assay reagent.

(10) Smoothelin-like 2 Inhibits Coronin-1B to Stabilize the Apical Actin Cortex during Epithelial MorphogenesisCurrent biology : CBFebruary 22, 2021Hachimi M, Grabowski C, Campanario S, Herranz G, Baonza G, Serrador JM, Gomez-Lopez S, Barea MD, Bosch-Fortea M, Gilmour D, et al.at 4°C for 30 min. The supernatant was incubated with 1.25 ml Profinity™ IMAC resin Ni-charged (Cat# 1560133, Bio-Rad) at 4°C for 1–2 h. The resin was loaded into an open PD-10 column (Cat# GE17043501, Miillipore-Sigma)

(11) Bamboo Shark as a Small Animal Model for Single Domain Antibody ProductionFrontiers in bioengineering and biotechnologyJanuary 1, 2021Wei L, Wang M, Xiang H, Jiang Y, Gong J, Su D, Al Azad MAR, Dong H, Feng L, Wu J, et al.0.22 µm syringe filters (Merck, SLGS033SB) and then added with 1 ml of IMAC nickel resin (Bio-Rad, 1560135) for affinity capture of His-tagged nanobodies. After an overnight gentle shaking at 4°C, the nickel

(12) Dissecting the role of a plant-specific Rab5 small GTPase NbRabF1 in Bamboo mosaic virus infectionJournal of experimental botanyDecember 31, 2020Huang YP, Hou PY, Chen IH, Hsu YH, Tsai CH, Cheng CP.extract was clarified by centrifugation at 17 418 ×g for 5 min, then incubated with 1 ml High-Capacity Profinity™ IMAC Resins (Bio-Rad, CA, USA) at 4 °C for 1 h, followed by washing with 10 ml buffer A containing 25 mM imidazole,

(13) Purification of a heterodimeric Reelin construct to investigate binding stoichiometryEuropean Biophysics JournalDecember 1, 2020Liam S. Turk, Daniel Mitchell, Davide Comolettithis, we repeated the experiment in reverse order, performing an IP using IMAC Ni2+ resin (Bio-Rad 1560133), then probing for the FLAG tag (Sigma F3165). As expected, we obtained a band at 160 kDa when using

(14) An ECF-type transporter scavenges heme to overcome iron-limitation in Staphylococcus lugdunensiseLifeJune 9, 2020Angelika Jochim et al.(TMBZ)",Sigma Aldrich,860336, "Chemical compound, drug",Profinity IMAC resin nickel chrged,BIO RAD,1560135,

(15) Von Willebrand Factor Type A domain of hCLCA1 is sufficient for U-937 macrophage activationBiochemistry and Biophysics ReportsJuly 1, 2019Brandon A. Keith, John C.H. Ching, Matthew E. Loewenlysed by sonication. The soluble fraction was loaded onto a Ni2+-NTA (Bio-Rad Profinity IMAC resin. 1560123; Biorad) affinity column and eluted with a gradient of 20–300 mM imidazole. Eluted fractions were pooled

(16) Regulation of HLTF-mediated PCNA polyubiquitination by RFC and PCNA monoubiquitination levels determines choice of damage tolerance pathwayNucleic acids researchNovember 30, 2018Masuda Y, Mitsuyuki S, Kanao R, Hishiki A, Hashimoto H, Masutani C.PCNA with histidine-tagged proteins, a 3 μl suspension of Profinity™ IMAC Ni-Charged Resin (BIO-RAD, #1560131) was resuspended in 10 μl of binding buffer containing 20 mM HEPES–NaOH (pH 7.5), 50 mM NaCl, 10 mM

(17) Purification of Soluble Recombinant Human Tau Protein from Bacteria Using Double-tag Affinity PurificationBIO-PROTOCOLNovember 20, 2018Joseph McInnes, Lujia Zhou, And Patrik Verstrekenafter thawing. 16. Ni-NTA ProfinityTM IMAC Resin, Ni-charged (Bio-Rad Laboratories, catalog number: 1560131) 17. Quick StartTM Bradford Protein Assay (Bio-Rad Laboratories, catalog number: 5000201) 18. PageBlueTM

(18) A peptide tag-specific nanobody enables high-quality labeling for dSTORM imagingNature communicationsMarch 2, 2018Virant D, Traenkle B, Maier J, Kaiser PD, Bodenhöfer M, Schmees C, Vojnovic I, Pisak-Lukáts B, Endesfelder U, Rothbauer U.incubated for 5 h at 25 °C. Uncoupled nanobody and sortase were depleted using Ni-NTA resin (Biorad, cat. #1560131). Unbound dye was removed using Zeba Spin Desalting Columns (ThermoFisher Scientific, cat. #89890).

(19) A Novel TetR-Like Transcriptional Regulator Is Induced in Acid-Nitrosative Stress and Controls Expression of an Efflux Pump in MycobacteriaFrontiers in MicrobiologyOctober 23, 2017Filomena Perrone, Barbara De Siena, Lidia Muscariello, Sharon L. Kendall, Simon J. Waddell, Margherita SaccomM IPTG for 2 h at 37°C and harvested by centrifugation. The recombinant protein was purified using Profinity IMAC resins (Bio-Rad) following manufacturer’s instructions and concentrated using Centricon ultra-filtration spin

(20) Nuclear import of Nkx2-2 is mediated by multiple pathwaysBiochemical and biophysical research communicationsJanuary 22, 2017Lin W, Xu P, Guo Y, Jia Q, Tao T.isolated using glutathione Sepharose 4B beads (GST beads, #6200226, Bio-Rad, USA) or Ni-NTA beads(#1560131, Bio-Rad, USA) according to the manufacturer’s instructions. Purified proteins were desalted with a

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