UltraCruz® Protease Inhibitor Cocktail Tablet, EDTA-free Chemicals from Santa Cruz Biotechnology, Inc.

Product Specs
ItemUltraCruz® Protease Inhibitor Cocktail Tablet, EDTA-free Chemicals
CompanySanta Cruz Biotechnology, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Numbersc-29131
Quantity20 tablets
Shipped4° C
TypeBiochemical Reagents

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) miR-210 Expression Is Strongly Hypoxia-Induced in Anaplastic Thyroid Cancer Cell Lines and Is Associated with Extracellular Vesicles and Argonaute-2International Journal of Molecular SciencesFebruary 24, 2023Bonita H. Powell, Andrey Turchinovich, Yongchun Wang, Olesia Gololobova, Dominik Buschmann, Martha A. Zeiger, Christopher B. Umbricht, Kenneth W. Witwerice-cold 1X RIPA buffer (Cell Signaling 9806S, Danvers, MA, USA) with 1X Protease inhibitors (Santa Cruz sc-29131, Santa Cruz, CA, USA). Cells were incubated on ice for 10 min then transferred to tubes using cell scrapers.

(2) Brown adipose tissue dysfunction promotes heart failure via a trimethylamine N-oxide-dependent mechanismScientific ReportsSeptember 1, 2022Yohko Yoshida et al.Triton X-100, 1% deoxycholate, 0.1% SDS, and 1 × Complete Protease Inhibitor Cocktail (Santa Cruz, sc-29131)). The lysates (25–50 μg) were resolved by SDS-PAGE and proteins were transferred to PVDF membranes

(3) miRNA profiling of primate cervicovaginal lavage and extracellular vesicles reveals miR‐186‐5p as a potential antiretroviral factor in macrophagesFEBS Open BioOctober 1, 2020Zezhou Zhao, Dillon C. Muth, Kathleen Mulka, Zhaohao Liao, Bonita H. Powell, Grace V. Hancock, Kelly A. Metcalf Pate, Kenneth W. WitwerDanvers, MA, USA. Cat #: 9806S) and protease inhibitor (Santa Cruz Biotechnology, Dallas, TX, USA. Cat #: sc29131). Eight microliter of Laemmli 4× sample buffer (Bio‐Rad, Hercules, CA, USA. Cat #:161‐0747 Lot #: 64077737)

(4) Identification of Autophagy-related Proteins in Lungs From Hypersensitivity Pneumonitis PatientsJournal of Histochemistry & CytochemistryJune 5, 2020Sandra Cabrera, Carolina Rodríguez-Bobadilla, Dulce Vázquez-Morales, Miguel Gaxiola, Mariana Maciel, Moisés Selman, Annie PardoNaCl, 1% Triton X-100 (T8787; Sigma-Aldrich), 10 mM EDTA, and UltraCruz Protease Inhibitor Cocktail (sc-29131; Santa Cruz Biotechnology, Dallas, TX). Tissue and cell extracts were centrifuged at 15,000 × g at 4°C

(5) Translational derepression of Elavl4 isoforms at their alternative 5' UTRs determines neuronal developmentNature communicationsApril 3, 2020Popovitchenko T, Park Y, Page NF, Luo X, Krsnik Z, Liu Y, Salamon I, Stephenson JD, Kraushar ML, Volk NL, et al.lysis in PEB, freshly supplemented with EDTA-free protease inhibitor (Santa Cruz Biotechnology; no. sc-29131), RNaseOut (Invitrogen; no. 100000840), 20 mM DTT (Invitrogen; no. NP0009), and 0.1 mg/mL cyclohexamide

(6) Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell ProliferationInternational journal of molecular sciencesDecember 13, 2019Ionescu AE, Mentel M, Munteanu CVA, Sima LE, Martin EC, Necula-Petrareanu G, Szedlacsek SE.Diego, CA, USA), NP-40 (A1694) from Panreac AppliChem (Germany), protease inhibitor cocktail tablets (sc-29131), and sodium orthovanadate (sc-3540) from Santa Cruz Biotechnology, USA, iodoacetic acid (35603, Pierce™,

(7) AMPK and PPARβ positive feedback loop regulates endurance exercise training-mediated GLUT4 expression in skeletal muscleAmerican Journal of Physiology-Endocrinology and MetabolismMay 1, 2019Jin-Ho Koh, Chad R. Hancock, Dong-Ho Han, John O. Holloszy, K. Sreekumaran Nair, Surendra DasariRIPA buffer supplemented with protease inhibitor cocktail tablet (Santa Cruz Biotechnology, cat. no. SC29131, Santa Cruz, CA). Protein concentration was measured as previously described (23). Antibodies used are

(8) Impaired autophagic activity and ATG4B deficiency are associated with increased endoplasmic reticulum stress-induced lung injuryAgingAugust 27, 2018Maciel M, Hernández-Barrientos D, Herrera I, Selman M, Pardo A, Cabrera S.(Sigma-Aldrich, T8787), 10 mM EDTA and UltraCruz Protease Inhibitor Cocktail (Santa Cruz Biotechnology, sc-29131). Tissue and cell extracts were centrifuged at 15,000 × g at 4°C and supernatant fractions were collected.

(9) The mre11 A470 alleles influence the hereditability and the segregation of telosomes in Saccharomyces cerevisiaePLOS ONESeptember 8, 2017In-Joon Baek, Daniel S. Moss, Arthur J. LustigNaCl, 1 mM EDTA, 1% Triton X-100, DOC [0.1%], SDS [0.1%] and a protease inhibitor cocktail [Santa Cruz; SC29131] dissolved in 30 μl TE. Real-Time PCR ChIP analysis of Mre11 abundance in telomeric chromatin Cells

(10) Defective Sphingosine-1-phosphate metabolism is a druggable target in Huntington’s diseaseScientific ReportsJuly 13, 2017Alba Di Pardo, Enrico Amico, Abdul Basit, Andrea Armirotti, Piyush Joshi, Diana M. Neely, Romina Vuono, Salvatore Castaldo, Anna F. Digilio, Francesco Scalabrì, Giuseppe Pepe, Francesca Elifani, Michele Madonna, Se Kyoo Jeong, Bu-Mahn Park, Maurizio D’Esposito, Aaron B. Bowman, Roger A. Barker, Vittorio MaglioneNonidet P-40, 1 mM EDTA, 20 mMNaF, 2 mM Na3V04, and protease inhibitor mixture (Santa Cruz, Cat N. sc-29131) and sonicated with 2 × 10 s pulses. Immunohistochemistry. Deparaffinized striatal and cortical tissue

(11) Amyotrophic lateral sclerosis patient iPSC-derived astrocytes impair autophagy via non-cell autonomous mechanismsMolecular brainJune 13, 2017Madill M, McDonagh K, Ma J, Vajda A, McLoughlin P, O'Brien T, Hardiman O, Shen S.Protein was harvested using RIPA buffer (Sigma, R0278) supplemented with protease inhibitors (Santa Cruz, sc-29131). Protein concentration was calculated using BCA kit (Thermo Fisher Cat #23225). 10ug of protein was

(12) PPARβ Is Essential for Maintaining Normal Levels of PGC-1α and Mitochondria and for the Increase in Muscle Mitochondria Induced by ExerciseCell metabolismMay 2, 2017Koh JH, Hancock CR, Terada S, Higashida K, Holloszy JO, Han DH.sodium pyrophosphate) supplemented with protease inhibitor cocktail tablet (Santa Cruz Biotechnology #SC-29131, Santa Cruz, CA, USA). The homogenates were frozen and thawed 3 times to disrupt the mitochondria. The

(13) The STIM1-binding site nexus remotely controls Orai1 channel gatingNature CommunicationsDecember 8, 2016Yandong Zhou, Xiangyu Cai, Natalia A. Loktionova, Xianming Wang, Robert M. Nwokonko, Xizhuo Wang, Youjun Wang, Brad S. Rothberg, Mohamed Trebak, Donald L. GillNaCl, 10 mM Tris-HCl (pH 7.4), 1% NP-40, with one tablet of complete protease inhibitors (Santa Cruz, sc-29131) per 25 ml. Lysis occurred on ice for 30 min, followed by centrifugation at 14,000 g for 10 min at 4

(14) TRPM7 Regulates Axonal Outgrowth and Maturation of Primary Hippocampal NeuronsMolecular NeurobiologyJanuary 1, 2016Ekaterina Turlova, Christine Y. J. Bae, Marielle Deurloo, Wenliang Chen, Andrew Barszczyk, F. David Horgen, Andrea Fleig, Zhong-Ping Feng, Hong-Shuo Sunbuffer (Santa Cruz Biotechnology, sc-24948) with protease inhibitor mixture (Santa Cruz Biotechnology, sc-29131). The protein samples (15 μg/well) were run on 8 % Tris-glycine gel. Membranes were incubated with specific

(15) STIM1 dimers undergo unimolecular coupling to activate Orai1 channelsNature CommunicationsDecember 1, 2015Yandong Zhou, Xizhuo Wang, Xianming Wang, Natalia A. Loktionova, Xiangyu Cai, Robert M. Nwokonko, Erin Vrana, Youjun Wang, Brad S. Rothberg, Donald L. GillNaCl, 10 mM Tris-HCl (pH 7.4), 1% NP-40 and one tablet of complete protease inhibitors (Santa Cruz, sc-29131) per 25 ml. Lysis was on ice for 30 min, followed by centrifugation at 14,000g, 4 °C for 10 min. Supernatant

(16) Thalamic WNT3 Secretion Spatiotemporally Regulates the Neocortical Ribosome Signature and mRNA Translation to Specify Neocortical Cell SubtypesThe Journal of neuroscience : the official journal of the Society for NeuroscienceAugust 5, 2015Kraushar ML, Viljetic B, Wijeratne HR, Thompson K, Jiao X, Pike JW, Medvedeva V, Groszer M, Kiledjian M, Hart RP, et al.in T-PER (Thermo Scientific #78510) supplemented with protease inhibitor (Santa Cruz Biotechnology #sc29131) in n ⫽ 3 cultures/condition. Plasmids and cloning. The full-length Foxp2–3⬘UTR was amplified by PCR

(17) Temporally defined neocortical translation and polysome assembly are determined by the RNA-binding protein Hu antigen RProceedings of the National Academy of SciencesSeptember 9, 2014Matthew L. Kraushar, Kevin Thompson, H. R. Sagara Wijeratne, Barbara Viljetic, Kristina Sakers, Justin W. Marson, Dimitris L. Kontoyiannis, Steven Buyske, Ronald P. Hart, Mladen-Roko RasinmM NaCl, 10 mM MgCl2) supplemented with EDTA-free protease inhibitor (Santa Cruz Biotechnology; no. sc-29131), RNase inhibitor (Invitrogen; no. 100000840), 20 mM DTT (Invitrogen; no. NP0009), and 0.1 mg/mL cyclohexamide

(18) Distinct Orai-coupling domains in STIM1 and STIM2 define the Orai-activating siteNature communicationsJanuary 1, 2014Wang X, Wang Y, Zhou Y, Hendron E, Mancarella S, Andrake MD, Rothberg BS, Soboloff J, Gill DL.10mM Tris-HCl (pH 7.4), 1% Igepal Ca-360, and one tablet of complete protease inhibitors (Santa Cruz, sc-29131) per 25 ml. For 2-APB treatment, lysis buffer was supplemented with 50 µM 2-APB. Cell lysates were centrifuged

(19) Susceptibility of juvenile and adult blood-brain barrier to endothelin-1: regulation of P-glycoprotein and breast cancer resistance protein expression and transport activityJournal of neuroinflammationDecember 19, 2012Rania Harati, Anne-Sophie Villégier, William A Banks, Aloise Mabondzo

(20) Susceptibility of juvenile and adult blood-brain barrier to endothelin-1: regulation of P-glycoprotein and breast cancer resistance protein expression and transport activityJournal of neuroinflammationDecember 19, 2012Rania Harati, Anne-Sophie Villégier, William A Banks, Aloise Mabondzo7.4, 0.15 M NaCl, 2 mM EDTA, 1 mM EGTA with protease inhibitor cocktail (Santa Cruz Biotechologies, sc-29131)) was added to each tube. Samples were centrifuged (100 g) for 10 minutes at 4°C, and then the supernatant

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UltraCruz® Protease Inhibitor Cocktail Tablet, EDTA-free Chemicals from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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UltraCruz® Protease Inhibitor Cocktail Tablet, EDTA-free Chemicals from Santa Cruz Biotechnology, Inc.

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