ProLong ® Gold Antifade Reagent with DAPI from Cell Signaling Technology

Product Specs
ItemProLong ® Gold Antifade Reagent with DAPI
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number8961S
Quantity10 ml
Research AreaRelated Products
ApplicationsIF-P, IF-F, IF-IC

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Discovery of potent tubulin inhibitors targeting the colchicine binding site via structure-based lead optimization and antitumor evaluationJournal of Enzyme Inhibition and Medicinal ChemistryDecember 31, 2023Wei Liu, Youyou He, Zhongjie Guo, Miaomiao Wang, Xiaodong Han, Hairui Jia, Jin He, Shanshan Miao, Shengzheng Wangthe microtubules. The nucleus was visualising using DAPI with prolong gold antifade reagent (CST, #8961). The photographs of cells were captured using the Zeiss 810 Confocal microscope. In vivo antitumour

(2) Protocol for the production and purification of an i-Motif-specific nanobodySTAR ProtocolsDecember 1, 2023Mahdi Zeraati, Samuel E. Ross, Behnaz Aghaei, Alvaro González Rajal, Cecile King, Marcel E. Dingerw/v),Sigma-Aldrich,Cat# 11332481001 ProLong Gold antifade reagent with DAPI,Cell Signaling Technology,Cat# 8961S Alexa Fluor 555 conjugated phalloidin (1:20 dilution for immunostaining),Cell Signaling Technology,Cat#

(3) Detection of isoforms and genomic alterations by high-throughput full-length single-cell RNA sequencing in ovarian cancerNature CommunicationsNovember 27, 2023Arthur Dondi, Ulrike Lischetti, Francis Jacob, Franziska Singer, Nico Borgsmüller, Ricardo Coelho, Rudolf Aebersold, Melike Ak, Faisal S. Al-Quaddoomi, Silvana I. Albert, et al.Slides were mounted using ProLong® Gold Antifade Reagent with DAPI (Cell Signaling Technology, cat. no. 8961). Images were acquired using a Nikon spinning-disk confocal microscope (Nikon CSU-W1 spinning-disk confocal

(4) Increased intrinsic membrane excitability is associated with hypertrophic olivary degeneration in spinocerebellar ataxia type 1bioRxivOctober 28, 2023Logan M. Morrison, Haoran Huang, Hillary P. Handler, Min Fu, David D. Bushart, Samuel S. Pappas, Harry T. Orr, Vikram G. Shakkottaimounted in this square using ProLong Gold Antifade Mountant with DAPI (Cell Signaling Technology, Cat# 8961S), with specific attention being paid to the notch in the section to ensure that the surface of the section

(5) Acquired resistance to KRAS G12C small-molecule inhibitors via genetic/nongenetic mechanisms in lung cancerScience AdvancesOctober 13, 2023Atish Mohanty, Arin Nam, Saumya Srivastava, Jeff Jones, Brett Lomenick, Sharad S. Singhal, Linlin Guo, Hyejin Cho, Aimin Li, Amita Behal, et al.washing at room temperature. ProLong Antifade 4′,6-diamidino-2-phenylindole mounting medium (catalog no. 8961, Cell Signaling Technology, USA) was used for mounting the coverslips and imaged with Zeiss LSM 880

(6) Chronological adhesive cardiac patch for synchronous mechanophysiological monitoring and electrocoupling therapyNature CommunicationsOctober 6, 2023Chaojie Yu, Mingyue Shi, Shaoshuai He, Mengmeng Yao, Hong Sun, Zhiwei Yue, Yuwei Qiu, Baijun Liu, Lei Liang, Zhongming Zhao, et al.detect revascularization. Wheat germ agglutinin (WGA, W11262, Thermo Fisher, dilution 1:400) and DAPI (8961, Cell Signaling Technology) were used to stain the cell membrane and nucleus. The fluorescent secondary

(7) Curculigoside mitigates dextran sulfate sodium‑induced colitis by activation of KEAP1‑NRF2 interaction to inhibit oxidative damage and autophagy of intestinal epithelium barrierInternational Journal of Molecular MedicineSeptember 28, 2023Fang Li, Hua Huang, Ping Zhao, Jie Jiang, Xufeng Ding, Donxgue Lu, Lijiang Jithen added to the slides for 1 h at room temperature. Add ProLong® Gold Antifade Reagent with DAPI (#8961, Cell Signaling Technology) or Hoechst (#62249, Thermo Fisher Scientific), and incubate at room temperature

(8) Girdin regulates both migration and angiogenesis in pancreatic cancer cell linesOncology reportsSeptember 1, 2023Hayashi Y, Matsuo Y, Denda Y, Nonoyama K, Murase H, Ueda G, Aoyama Y, Kato T, Omi K, Imafuji H, et al.under shading, and DAPI for nuclear staining (blue) [ProLong® Gold Antifade Reagent with DAPI (cat. no. 8961; Cell Signaling Technology, Inc.) was applied a few drops per slide]. Fluorescence intensities were

(9) Cell type-specific delivery by modular envelope designNat CommunAugust 23, 2023Daniel Strebinger, Chris J. Frangieh, Mirco J. Friedrich, Guilhem Faure, Rhiannon K. Macrae, Feng Zhangcoverslips (VWR Cat. # 48393-221) using DAPI-containing mounting media (Cell Signaling Technology Cat. # 8961S). Coverslips were sealed with nail polish. Images were acquired on a Leica DMI8 Confocal Microscope

(10) A sex-specific thermogenic neurocircuit induced by predator smell recruiting cholecystokinin neurons in the dorsomedial hypothalamusNat CommunAugust 15, 2023Predrag Jovanovic, Allan-Hermann Pool, Nancy Morones, Yidan Wang, Edward Novinbakht, Nareg Keshishian, Kaitlyn Jang, Yuki Oka, Celine E. Rierathree times in PBS, mounted onto slides, coverslipped with ProLong® Gold Antifade Reagent with DAPI (8961 S, Cell Signaling). For cFos quantification, ROI sections were stained with a cFos antibody (1:1000,

(11) The dynamic expression of SOX17 in germ cells from human female foetus and adult ovaries after specificationFront Endocrinol (Lausanne)July 28, 2023Ying-Yi Luo, Hui-Ying Jie, Ke-Jun Huang, Bing Cai, Xiu Zhou, Ming-Yi Liang, Can-Quan Zhou, Qing-Yun MaiAlexa Fluor 647, Abcam). The sections were then stained with Prolong Gold Antifade Reagent with DAPI (#8961, CST, USA). Fluorescent images were captured using a Leica TCS-SP8 laser scanning confocal microscope

(12) Pharmacologic inhibition of glycolysis prevents the development of lupus by altering the gut microbiome in miceiScienceJuly 21, 2023Ahmed S. Elshikha, Yong Ge, Josephine Brown, Nathalie Kanda, Mojgan Zadeh, Georges Abboud, Seung-Chul Choi, Gregg Silverman, Timothy J. Garrett, William L. Clapp, et al.,BD Biosciences,550583 Fixation/Permeabilization kit,eBioscience,00-5123-43 ProLong Gold Antifade Reagent with DAPI,Cell Signaling,8961 ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models:

(13) Elevated PDGF-BB from Bone Impairs Hippocampal Vasculature by Inducing PDGFRβ Shedding from PericytesAdvanced science (Weinheim, Baden-Wurttemberg, Germany)July 1, 2023Liu G, Wang J, Wei Z, Fang CL, Shen K, Qian C, Qi C, Li T, Gao P, Wong PC, et al.ImmunoResearch Laboratories) for 1 to 4 h while avoiding light. The sections were then mounted with DAPI (8961S, Cell signaling Technology). The sample images were captured by a confocal microscope (Zeiss LSM 780). Vessel

(14) The NO Answer for Autism Spectrum DisorderAdvanced ScienceMay 22, 2023Manish Kumar Tripathi, Shashank Kumar Ojha, Maryam Kartawy, Wajeha Hamoudi, Ashwani Choudhary, Shani Stern, Adi Aran, Haitham AmalHRP‐conjugated anti‐rabbit (7076S), HRP‐conjugated anti‐mouse (7074S), ProLong gold Antifade with DAPI (#8961), and protease phosphatase inhibitor cocktail (#5872) were purchased from Cell Signaling Technology

(15) Diospyros lotus leaf extract and its main component myricitrin regulate pruritus through the inhibition of astrocyte activationExperimental and Therapeutic MedicineMay 16, 2023Jae Young Shin, Byoung Ok Cho, Ji Hyeon Park, Eun Seo Kang, Young Soo Kim, Seon Il Jangkit (ab245880) was purchased from Abcam. ProLong® gold antifade reagent with DAPI mounting solution (8961) was came from Cell signaling (Danvers, MA, USA). Cell culture Mouse-origin astrocytes (CRL-2535™)

(16) Molecular mechanisms of stress-induced reactivation in mumps virus condensatesCellApril 27, 2023Xiaojie Zhang, Sindhuja Sridharan, Ievgeniia Zagoriy, Christina Eugster Oegema, Cyan Ching, Tim Pflaesterer, Herman K.H. Fung, Isabelle Becher, Ina Poser, Christoph W. Müller, et al.7784-41-0 "1,6-Hexanediol",Sigma-Aldrich,240117; CAS: 629-11-8 ProLong Gold Antifade Reagent with DAPI,Cell Signaling Technology,Cat# 8961 Oligo d(T)23 primer,New England Biolabs,Cat#S1327S EDTA-free protease inhibitor cocktail,Roche,04693132001 PhosSTOP

(17) Protocol Optimization for Direct Reprogramming of Primary Human Fibroblast into Induced Striatal NeuronsInternational Journal of Molecular SciencesApril 5, 2023Nina Kraskovskaya, Anastasia Bolshakova, Mikhail Khotin, Ilya Bezprozvanny, Natalia Mikhailovawere fixed on a slide using Mounting Medium with DAPI (Cell Signalling, Danvers, MA, USA, Cat. No. 8961S). Images were captured with Olympus Fluoview FV3000 laser confocal system. 4.7. Ca2+ Imaging Fluo-4

(18) Quercetin Alleviates Pulmonary Fibrosis in Silicotic Mice by Inhibiting Macrophage Transition and TGF-β-Smad2/3 PathwayCurrent Issues in Molecular BiologyApril 5, 2023Fei Geng, Lan Zhao, Yuhao Cai, Ying Zhao, Fuyu Jin, Yaqian Li, Tian Li, Xinyu Yang, Shifeng Li, Xuemin Gao, et al.(A16028 and A16018, Novex, Frederick, MD, USA) (1:100) for 40 min. The nuclei were stained with DAPI (8961s; Cell Signaling Technology, Inc., Danvers, MA, USA). The sections were examined under an inverted fluorescence microscope

(19) Present in the Aquatic Environment, Unclear Evidence in Top Predators—The Unknown Effects of Anti-Seizure Medication on Eurasian Otters (Lutra lutra) from Northern GermanyToxicsMarch 31, 2023Simon Rohner, Martina Gramer, Ivo Wiesweg, Oliver Scherf-Clavel, Peter Wohlsein, Martin Schmelz, Ursula Siebert, Franziska Richter, Manuela Gernert(triton ×100). After covering with ProLong™ Gold Antifade Mountant with DAPI (Cell Signaling Tech. 8961S), the sections were digitized with a Zeiss Axio Observer 7 microscope and associated computer program

(20) Ellagitannins from Castanea sativa Mill. Leaf Extracts Impair H. pylori Viability and Infection-Induced Inflammation in Human Gastric Epithelial CellsNutrientsMarch 21, 2023Stefano Piazza, Giulia Martinelli, Marco Fumagalli, Carola Pozzoli, Nicole Maranta, Flavio Giavarini, Luca Colombo, Giovanna Nicotra, Silvia Francesca Vicentini, Francesca Genova, et al.on slides with a drop of ProLong Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole (DAPI) (#8961, Cell Signaling Technology, Danvers, MA, USA), and then imaged using a confocal laser scanning microscope

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