Nunc(TM) MaxiSorp(TM) ELISA Plates, Uncoated from BioLegend

Product Specs
ItemNunc(TM) MaxiSorp(TM) ELISA Plates, Uncoated
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number423501
Quantity5 Plates
ApplicationsELISA

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) An Animal Model for Chronic Meningeal Inflammation and Inflammatory Demyelination of the Cerebral CortexInternational journal of molecular sciencesSeptember 9, 2023Enz LS, Winkler A, Wrzos C, Dasen B, Nessler S, Stadelmann C, Schaeren-Wiemers N.diluted in coating buffer (421701, BioLegend) and 100 μL per well was distributed in a 96-well plate (423501, Biolegend). After 18 h of incubation at 4 °C, wells were washed with 0.05% Tween 20 in PBS three times

(2) NCF4 dependent intracellular reactive oxygen species regulate plasma cell formationRedox BiologyOctober 1, 2022Chang He, Huqiao Luo, Ana Coelho, Meng Liu, Qijing Li, Jing Xu, Alexander Krämer, Stephen Malin, Zuyi Yuan, Rikard Holmdahlby ELISA. Briefly, serum was diluted and added to COL2-coated (10 μg/ml) ELISA plates (Biolegend, #423501). Bound Igs were detected with HRP-conjugated goat anti-mouse Ig kappa (Southern Biotech, #1170-05)

(3) Neuroprotective Effect of Morin Hydrate against Attention-Deficit/Hyperactivity Disorder (ADHD) Induced by MSG and/or Protein Malnutrition in Rat Pups: Effect on Oxidative/Monoamines/Inflammatory Balance and ApoptosisPharmaceuticals (Basel)August 17, 2022Hoda A. Salem et al.acidic protein (GFAP) (Cat.# NS830, Sigma-Aldrich, Burlington, MA, USA), interleukin-1β (IL-1β) (Cat.# 423501, BioLegend, San Diego, CA, USA) and brain derived neurotrophic factor (BDNF) (Cat.# BEK-2211-CE, Biosensis,

(4) A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasmaNature Biomedical EngineeringAugust 8, 2022Devora Najjar, Joshua Rainbow, Sanjay Sharma Timilsina, Pawan Jolly, Helena de Puig, Mohamed Yafia, Nolan Durr, Hani Sallum, Galit Alter, Jonathan Z. Li, et al.REC31849) were prepared in a 10 mM PBS buffer at pH 7.4, added to Nunc MaxiSorp ELISA plates (BioLegend, 423501) and immobilized on the plates by overnight incubation at 4 °C. The plates were washed three times with

(5) Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cellsSTAR ProtocolsJune 9, 2022Chia-Hung Christine Hsiao, Andrew J. WiemermM,Corning,Cat#MT25000CI Plate Sealer,Thermo Scientific,Cat#3501 "Nunc MaxiSorp ELISA Plates, Uncoated",BioLegend,Cat#423501 Strip tubes,DOT Scientific,Cat#403-8PCR 500 μL cryovials,Nunc,Cat#12570216 Flat bottom 96 well plate,DOT

(6) Function of ceramide transfer protein for biogenesis and sphingolipid composition of extracellular vesiclesJournal of Extracellular VesiclesJune 1, 2022Simone M. Crivelli et al.CERT‐Tsg101 complex and complex modelling with AlphaFold2 in ColabFold ELISA. Maxisorp plates (Nunc, 423501, Biolegend) were coated with 2 μg/ml of recombinant human CERTL (rCERTL), produced as previously described

(7) Extracellular matrix proteins regulate NK cell function in peripheral tissuesScience AdvancesMarch 18, 2022Mark D. Bunting, Maulik Vyas, Marta Requesens, Adam Langenbucher, Erik B. Schiferle, Robert T. Manguso, Michael S. Lawrence, Shadmehr DemehriPK136, BioLegend, catalog no. 108703) precoated plate (Nunc MaxiSorp plate, BioLegend, catalog no. 423501) or treated with 1× PMA/Ion solution (BioLegend, catalog no. 423301). The cells were incubated at 37°C/5%

(8) The deubiquitinase Usp27x as a novel regulator of cFLIPL protein expression and sensitizer to death-receptor-induced apoptosisApoptosisFebruary 1, 2022Manuel Nico Dold, Xiulin Ng, Claudia Alber, Ian Edward Gentle, Georg Häcker, Arnim WeberDeluxe Set Human TNF-α, BioLegend: #430204) on uncoated 96 Nunc™ MaxiSorp™ ELISA Plates (BioLegend: #423501) in technical triplicates per biological sample. The range of the standard was modified from 7.8 to

(9) MEK6 Overexpression Exacerbates Fat Accumulation and Inflammatory Cytokines in High-Fat Diet-Induced ObesityInternational Journal of Molecular SciencesDecember 17, 2021Suyeon Lee, Myoungsook LeeBioLegend, San Diego, CA, USA) through the standard protocol. The unplates were 96-well plate (Cat #423501) coated overnight with a coating buffer (Cat #79008) containing IL-10 or TNF-α, and the 1X assay diluent

(10) A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodiesNature CommunicationsDecember 1, 2021Xun Chen, Matteo Gentili, Nir Hacohen, Aviv Regeva NanoDrop spectrophotometer. ELISA assay for nanobody binding to RBD Maxisorp plates (BioLegend, 423501) were coated with 1 µg/ml anti-Flag antibody (Sigma Aldrich, F1804) in coating buffer (BioLegend, 421701)

(11) Revisiting an IgG Fc Loss-of-Function Experiment: the Role of Complement in HIV Broadly Neutralizing Antibody b12 ActivitymBioOctober 26, 2021Goldberg BS, Kaku CI, Dufloo J, Bruel T, Schwartz O, Spencer DA, Hessell AJ, Ackerman ME.pH 9.6) and immobilized to clear high-protein-binding-capacity 96-well ELISA plates (catalog number 423501; BioLegend) overnight at 4°C. Plates were subsequently washed three times with PBS-T (1× PBS, 0.05%

(12) Trypanosoma brucei triggers a broad immune response in the adipose tissuePLOS PathogensSeptember 15, 2021Henrique Machado, Tiago Bizarra-Rebelo, Mariana Costa-Sequeira, Sandra Trindade, Tânia Carvalho, Filipa Rijo-Ferreira, Barbara Rentroia-Pacheco, Karine Serre, Luisa M. FigueiredoPortugal. Antibody quantification Antigen-specific antibody titers were determined by ELISA. Assay plates (423501, BioLegend) were coated overnight with 2 μg/mL purified VSG AnTat1.1 (10 mM sodium phosphate buffer,

(13) Bamboo Shark as a Small Animal Model for Single Domain Antibody ProductionFrontiers in bioengineering and biotechnologyJanuary 1, 2021Wei L, Wang M, Xiang H, Jiang Y, Gong J, Su D, Al Azad MAR, Dong H, Feng L, Wu J, et al.particles were incubated in each GFP-coated (or iRFP713-coated) well of MaxiSorp plate (BioLegend, 423501) for vNAR binding with GFP (or iRFP713) at RM for 2 h. The unbound phage particles were washed away

(14) Adaptable antibody Nanoworms designed for non-Hodgkin lymphomaBiomaterialsDecember 1, 2020Changrim Lee, Santosh Peddi, Caleb Anderson, Hao Su, Honggang Cui, Alan L. Epstein, J. Andrew MacKayT cell activation, 70 μL of 0.52 mg/mL OKT3 antibody solution was filled into wells in 96-well plate (#423501, BioLegend, San Diego, CA, USA), sealed, and incubated at 4 °C one day prior to the experiment (day

(15) A cell-free antibody engineering platform rapidly generates SARS-CoV-2 neutralizing antibodiesbioRxiv : the preprint server for biologyOctober 30, 2020Chen X, Gentili M, Hacohen N, Regev A.nm on a NanoDrop Spectrophotometer. ELISA assay for VHH binding to RBD Maxisorp plates (BioLegend, 423501) were coated with 1μg/ml anti-Flag antibody (Sigma Aldrich, F1804) in coating buffer (BioLegend, 421701)

(16) Destabilizing single chain major histocompatibility complex class I protein for repurposed enterokinase proteolysisScientific reportsSeptember 10, 2020Lim J.coating buffer (BioLegend, cat no. 421701) was coated onto Nunc Maxisorp ELISA plates (BioLegend, cat no. 423501) at 4 °C overnight. The next day, unbound anti-human β2m was washed out with 1xELISA wash buffer (BioLegend,

(17) In vivo synthesis of bacterial amyloid curli contributes to joint inflammation during S. Typhimurium infectionPLoS pathogensJuly 1, 2020Miller AL, Pasternak JA, Medeiros NJ, Nicastro LK, Tursi SA, Hansen EG, Krochak R, Sokaribo AS, MacKenzie KD, Palmer MB, et al.delivered in four doses subcutaneously. Detection of CsgA specific IgG Nunc Maxisorp plates (Biolegend, 423501) were coated with 1–5μg GST-CsgAR1-5 in Tris. HCL pH 8.0 and stored at 4˚C overnight. The plate was

(18) The novel llama-human chimeric antibody has potent effect in lowering LDL-c levels in hPCSK9 transgenic ratsClinical and translational medicineFebruary 13, 2020Li X, Wang M, Zhang X, Liu C, Xiang H, Huang M, Ma Y, Gao X, Jiang L, Liu X, et al.HepG2 cells were plated at a density of 5 × 105 cells/well in 96-well plates (BIOLEGEND, USA, Cat# 423501) in Dulbecco’s modified eagle medium (DMEM, GIBCO, USA, Cat#11965084) supplemented with 10% fetal bovine

(19) Functional equivalence of stem cell and stem cell-derived extracellular vesicle transplantation to repair the irradiated brainStem cells translational medicineJanuary 1, 2020Smith SM, Giedzinski E, Angulo MC, Lui T, Lu C, Park AL, Tang S, Martirosian V, Ru N, Chmielewski NN, et al.

(20) Biological Responses of Onion-Shaped Carbon NanoparticlesNanomaterials (Basel)July 15, 2019Jaehee Jang, Youngjun Kim, Jangsun Hwang, Yonghyun Choi, Masayoshi Tanaka, Eunah Kang, Jonghoon Choitransferred to each well of a high-affinity, protein-binding ELISA plate (for example BioLegend Cat. No. 423501). The plate was incubated at 4 °C overnight. After three washes with PBS/Tween, non-specific binding

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9/17/2019Great BioLegend Plates - Increased the Reproducibility of Our ELISA David TimmIf you want a good plate for your ELISA, look no further.

Nunc(TM) MaxiSorp(TM) ELISA Plates, Uncoated from BioLegend

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Nunc(TM) MaxiSorp(TM) ELISA Plates, Uncoated from BioLegend

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