NEB 5-alpha Competent E. coli (High Efficiency) from New England Biolabs

Product Specs
ItemNEB 5-alpha Competent E. coli (High Efficiency)
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberC2987H
Quantity20 x 0.05 ml
GenotypefhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia
SpeciesE. coli
Temperature-80 C
Cell TypeCompetent Cells

Specifications/Features

Transformation Efficiency:
1- 3 x 10⁹ cfu/µg

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Direct stimulation of de novo nucleotide synthesis by O-GlcNAcylationNature chemical biologyJanuary 1, 2024Chen L, Zhou Q, Zhang P, Tan W, Li Y, Xu Z, Ma J, Kupfer GM, Pei Y, Song Q, et al.(Fisher BioReagents, BP1427) to amplify plasmids. The strain of BL21 E. coli (New England Biolabs, C2987) was cultured at 16 °C in LB broth (Fisher BioReagents, BP1427) with 0.2–0.5 µM isopropyl β-d-1-thiogalactopyranoside

(2) Unveiling the domain-specific and RAS isoform-specific details of BRAF kinase regulationElifeDecember 27, 2023Tarah Elizabeth Trebino, Borna Markusic, Haihan Nan, Shrhea Banerjee, Zhihong Wangbackground (Escherichia coli)",BL21 codon +,NEB,, "Strain, strain background (Escherichia coli)",DH5α,NEB,C2987I, Antibody,Anti-GST (mouse monoclonal),Santa Cruz Biotechnology,SC-138,WB (1:5000) Antibody,Anti-RAS

(3) Early-life stress triggers long-lasting organismal resilience and longevity via tetraspaninbioRxiv : the preprint server for biologyDecember 17, 2023Jiang WI, De Belly H, Wang B, Wong A, Kim M, Oh F, DeGeorge J, Huang X, Guang S, Weiner OD, et al.assembly control. 5 μl of assembly preparations were transformed to NEB 5-alpha Competent E. coli (C2987H, NEB) by heat shock at exactly 42°C for exactly 30 seconds. Positive clones were verified by bacteria

(4) Solid-to-liquid phase transition in the dissolution of cytosolic misfolded-protein aggregatesiScienceDecember 15, 2023Alexis Tomaszewski, Rebecca Wang, Eduardo Sandoval, Jin Zhu, Jian Liu, Rong Listrains,Bacterial and virus strains ,, NEB 5-alpha Competent E. coli (High Efficiency),NEB,Cat #: C2987 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(5) The Restriction-Modification Systems of Clostridium carboxidivorans P7MicroorganismsDecember 12, 2023Patrick Kottenhahn, Gabriele Philipps, Boyke Bunk, Cathrin Spröer, Stefan JenneweinAnaerobic Workstation (Don Whitley Scientific, Herzlake, Germany). Escherichia coli NEB 5-alpha cells (C2987H; New England Biolabs, Ipswich, MA, USA) were used for plasmid propagation and were incubated at 30 °C

(6) Biomolecular condensates in fungi are tuned to function at specific temperaturesbioRxivNovember 30, 2023Benjamin M. Stormo, Grace A. McLaughlin, Logan K. Frederick, Ameya P. Jalihal, Sierra J Cole, Ian Seim, Fred S. Dietrich, Amy S. Gladfelterjet kit (K1231 Thermo Fisher Scientific, Waltham, MA, USA) and then transformed into competent cells (C2987, New England BioLabs, Ipswich, MA, USA). Individual colonies were grown overnight under selection and

(7) Systematic characterization of the local evolutionary space available to human PKR and vaccinia virus K3bioRxivNovember 25, 2023Michael J. Chambers, Sophia Scobell, Meru J. Sadhu1 μL of the Gibson Assembly reaction was transformed into 10 μL 5-alpha competent E. coli (NEB Cat#C2987I) and plated onto 10 cm LB-AMP plates (IPM Scientific Cat#11006–016) and incubated overnight at 37°C.

(8) Parallel laboratory evolution and rational debugging reveal genomic plasticity to S. cerevisiae synthetic chromosome XIV defectsCell GenomicsNovember 8, 2023Thomas C. Williams, Heinrich Kroukamp, Xin Xu, Elizabeth L.I. Wightman, Briardo Llorente, Anthony R. Borneman, Alexander C. Carpenter, Niel Van Wyk, Felix Meier, Thomas R.V. Collier, et al.strains,Bacterial and virus strains ,, NEB 5-alpha Competent E. coli (High Efficiency),New England Biolabs,C2987H ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(9) Gene recoding by synonymous mutations creates promiscuous intragenic transcription initiation in mycobacteriamBioOctober 31, 2023Nuri K. Hegelmeyer, Lia A. Parkin, Mary L. Previti, Joshua Andrade, Raditya Utama, Richard J. Sejour, Justin Gardin, Stephanie Muller, Steven Ketchum, Alisa Yurovsky, et al.0.2% (wt/vol) casamino acid, 80 mg/L lysine, and 24 mg/L pantothenate. NEB5-alpha Competent E. coli (C2987, NEB) was used to propagate plasmids and was cultured in LB medium or grown on LB agar. All liquid cultures

(10) In-cell investigation of the conformational landscape of the GTPase UreG by SDSL-EPRiScienceOctober 20, 2023Annalisa Pierro, Ketty Concetta Tamburrini, Hugo Leguenno, Guillaume Gerbaud, Emilien Etienne, Bruno Guigliarelli, Valérie Belle, Barbara Zambelli, Elisabetta Mileocells,New England Bio Labs,C2527H Escherichia coli NEB® 5-alpha competent cells,New England Bio Labs,C2987H ,, Biological samples,Biological samples,Biological samples ,, We don’t used them in this study.,, ,, "Chemicals,

(11) Rare antibody phage isolation and discrimination (RAPID) biopanning enables identification of high-affinity antibodies against challenging targetsCommunications BiologyOctober 12, 2023Dong hee Chung, Sophie Kong, Nicholas J. Young, Shih-Wei Chuo, Jamie V. Shiah, Emily J. Connelly, Peter J. Rohweder, Alexandra Born, Aashish Manglik, Jennifer R. Grandis, et al.#E2611) methods. The Gibson assembly product was transformed into NEB® 5α Competent E. Coli cells (NEB #C2987H) and plasmids were isolated using a ZymoPureII Plasmid Maxiprep Kit (Zymo Research #D4203) and confirmed

(12) HIV-1 neutralizing antibodies provide sterilizing immunity by blocking infection of the first cellsCell Reports MedicineOctober 6, 2023Viktoria Stab, Christiane Stahl-Hennig, Armin Ensser, Elie Richel, Kirsten Fraedrich, Ulrike Sauermann, Bettina Tippler, Florian Klein, Dennis R. Burton, Matthias Tenbusch, et al.strains,Bacterial and virus strains ,, "DH5α E. coli strain, competent",New England Biolabs,Cat# C2987 "SIVdup (pseudotyped with SIV Env, HIV Env, gp120+SIV Env, VSV-G)",This paper,N/A SIVmac239,This paper,N/A ,, Biological

(13) Insights into substrate coordination and glycosyl transfer of poplar cellulose synthase-8StructureOctober 1, 2023Preeti Verma, Albert L. Kwansa, Ruoya Ho, Yaroslava G. Yingling, Jochen ZimmerCells,Novagen,Cat# 71400 NEB® 5-alpha Competent E. coli (High Efficiency),New England Biolabs,Cat# C2987 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(14) A Baculovirus Expression Vector Derived Entirely from Non-Templated, Chemically Synthesized DNA PartsVirusesSeptember 23, 2023Christopher Nguyen, Amanda Ibe-Enwo, Jeffrey Slackinstructions. Plasmids modified in vitro were transformed into chemically competent NEB® 5-alpha E. coli (#C2987H, New England Biolabs) according to the vendor’s instructions. Plasmids harboring subgenomic ‘A’ regions

(15) Rewiring the pneumococcal capsule pathway for investigating glycosyltransferase specificity and genetic glycoengineeringScience advancesSeptember 8, 2023Su T, Chua WZ, Liu Y, Fan J, Tan SY, Yang DW, Sham LT.were purified. The fragments were ligated by Gibson assembly and used to transform strain NEB5-α (NEB C2987) to produce pCS190 (PT7-his6-ggta1∆74). Transformants were selected for ampicillin resistance and screened

(16) A twin UGUA motif directs the balance between gene isoforms through CFIm and the mTORC1 signaling pathwayeLifeSeptember 4, 2023R Samuel Herron, Alexander K Kunisky, Jessica R Madden, Vivian I Anyaeche, May Z Maung, Hun-Way Hwangmonoclonal),Abcam,Cat#ab128874; RRID:AB_11145462,1:3000 "Strain, strain background",NEB 5-alpha,New England Biolabs,Cat#C2987, "Strain, strain background",NEB Stable,New England Biolabs,Cat#C3040, Commercial assay or kit,ProtoScript II First Strand cDNA Synthesis Kit

(17) Combining TSS-MPRA and sensitive TSS profile dissimilarity scoring to study the sequence determinants of transcription initiationNucleic Acids ResearchAugust 25, 2023Carlos Guzman, Sascha Duttke, Yixin Zhu, Camila De Arruda Saldanha, Nicholas L Downes, Christopher Benner, Sven Heinzbackbone) or NEB 5-alpha (plasmid backbone) chemically competent E.coli cells (50 μl; NEB C3040 or C2987, respectively) (see also Supplementary Note 2) were transformed with 1 μl Gibson assembly reaction according

(18) Dynamics of nuclear export of pre-ribosomal subunits revealed by high-speed single-molecule microscopy in live cellsiScienceAugust 18, 2023Samuel L. Junod, Mark Tingey, Joseph M. Kelich, Alexander Goryaynov, Karl Herbine, Weidong Yangand virus strains ,, NEB® 5-alpha Competent E. coli (High Efficiency),New England BioLabs,Cat #: C2987 BL21(DE3) Competent E. coli,New England BioLabs,Cat #: C2527 ,, "Chemicals, peptides, and recombinant

(19) Cell Free Bacteriophage Synthesis from Engineered Strains Improves YieldACS Synth BiolAugust 18, 2023Rani Brooks, Lisa Morici, Nicholas SandovalΦ80lacZΔM15Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rK–, mK+) phoA supE44 thi-1 gyrA96 relA1 λ-",NEB C2987I E. coli BL21,F–ompT lon hsdSB(rB– mB–) gal dcm [malB+]K-12(λS),ATCC BAA-1025 E. coli bacteriophage T7

(20) Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populationsSTAR ProtocAugust 15, 2023Miriam Merenciano, Laura Aguilera, Josefa Gonzálezstrains,Bacterial and virus strains,Bacterial and virus strains ,, 5-α competent E. coli cells,NEB,Cat # C2987H ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

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(1)

10/27/2021Great Competent Cells Wei WeiThese are great competent cells that have high transformation efficiency.