Description
Human peripheral blood leukopaks contain enriched mononuclear cells with minimal granulocytes and red blood cells. The mononuclear cells are composed of lymphocytes (T cells, B cells, and NK cells), monocytes, dendritic cells, and stem/progenitor cells. These cells are critical components of the immune system and play key roles in the humoral and cell-mediated immune responses. Mononuclear cells are commonly used in research and clinical applications in the areas of immunology, infectious disease, hematological malignancies, vaccine development, transplant therapy, and high-throughput screening.
Peripheral blood leukopaks are collected at our on-site Stem Cell Collection Center using the Spectra Optia® Apheresis System from healthy, IRB consented donors that are negative for HIV, HBV and HCV. After collection, leukopaks are checked by flow analysis to verify the percentage of granulocytes, monocytes, lymphocytes, and cell viability.
For the full leukopak, approximately three blood volumes are processed and between 200-300 mL of plasma can be collected at the same time as the mononuclear cells.
Donor Selection - 2000+ donors
- Recallable donors
- Specific donor demographics (i.e., age, weight, ethnicity, gender, blood type, etc.)
- Donor lifestyle characteristics (i.e., BMI, smoker/nonsmoker, etc.)
- Medical history (i.e. allergies, medications, etc.)
- Diseased donors
- HLA and ABO typing