LUNA-FL™ Dual Fluorescence Cell Counter from Logos Biosystems

Product Specs
ItemLUNA-FL™ Dual Fluorescence Cell Counter
CompanyLogos Biosystems
Price Get Pricing Get Quote for this Product
Catalog NumberL20001
Quantity1 unit
Dimensions(WxDxH) 22 x 21 x 9 cm (8.6 x 8.3 x 3.5 in)
MethodAutomated fluorescence and brightfield image-based cell detection (fluorescence staining & dye exclusion)
ColorGreen / Red
Analysis Time10 s (brightfield) 30 s (fluorescence)
Concentration1 x 10⁴ to 1 x 10⁷ cell/mL (optimal: 5 x 10⁴ to 1 x10⁷ cell/mL)
Cell Size Range1 to 90 µm (optimal: 5 to 60 µm)
SoftwareLuna™ Software
Electrical Requirements100 to 240 VAC, 50/60 Hz, 1.5 A
TypeAutomated cell counter
ApplicationsCell culture, cell counting, cell viability, quality control
DetectionAutomated dual fluorescence and brightfield microscopy
Sample TypeCell lines Primary cells Blood cells • PBMCs • Splenocytes • Thymocytes Yeast cells
Resolution800 X 480 pixels
Sample Volume10 µL
Interface7 in. capacitative touchscreen, 800 X 480 pixels
Weight1.8 kg (3.9 lb)
Components1 LUNA-FL™ Dual Fluorescence Cell Counter 1 box PhotonSlides™, 50 Slides 2 x 0.5 mL Acridine Orange/Propidium Iodide Stain 2 x 1 mL 0.4% Trypan Blue Stain

Specifications/Features

Image Size: 5 MP

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Logos Biosystems

FL3

28 Simindaero 327beon-gil, Dongan-gu

Anyang-si, Gyeonggi-do 14055

Korea, Republic of (South Korea)

Phone: +82-31-478-4185
Fax: +82 (31) 360-4277

Email: sales@logosbio.com

Company Profile

Website: www.logosbio.com

(1) Reusable Single Cell for Iterative Epigenomic AnalysesJournal of Visualized ExperimentsFebruary 28, 2022Hidetaka Ohnuki, David J. Venzon, Alexei Lobanov, Giovanna Tosatomg/mL),Thermo Fisher,AM9520,Linear Acrylamide LUNA-FL Dual Fluorescence Cell Counter,Logos Biosystems,L20001,Cell counter "LUNA™ Cell Counting Slides, 50 Slides",Logos Biosystems,L12001,Cell counter "Mineral oil

(2) Diverse human astrocyte and microglial transcriptional responses to Alzheimer's pathologyActa neuropathologicaJanuary 1, 2022Smith AM, Davey K, Tsartsalis S, Khozoie C, Fancy N, Tang SS, Liaptsi E, Weinert M, McGarry A, Muirhead RCJ, et al.resuspended in 50 μl PBS buffer, and counted on a LUNA-FL Dual Fluorescence Cell Counter (Logos Biosystems, L20001) using Acridine Orange dye to stain nuclei. Sufficient nuclei were added for a target of 7,000 nuclei

(3) Lentiviral and adeno-associated vectors efficiently transduce mouse T lymphocytes when targeted to murine CD8Molecular Therapy - Methods & Clinical DevelopmentDecember 10, 2021Alexander Michels, Annika M. Frank, Dorothee M. Günther, Mehryad Mataei, Kathleen Börner, Dirk Grimm, Jessica Hartmann, Christian J. Buchholzdetermined using the LUNA-FL Automated Fluorescence Cell Counter (Logos Biosystems, Annandale, VA, USA, L20001), LUNA Cell Counting Slides (Logos Biosystems, L12001), and acridine orange/propidium iodide dye (Logos Biosystems

(4) Transcriptional analysis of mouse wounds grafted with human mesenchymal stem cells and plateletsSTAR ProtocolsSeptember 17, 2021Jennyfer Levoux, Peggy Lafuste, Anne-Marie Rodriguez

(5) Cross-platform transcriptional profiling identifies common and distinct molecular pathologies in Lewy body diseasesActa NeuropathologicaSeptember 1, 2021Rahel Feleke et al.

(6) Exploiting evolutionary steering to induce collateral drug sensitivity in cancerNature CommunicationsDecember 1, 2020Ahmet Acar et al.acridine orange/propidium iodide dye with LUNA-FL Dual Fluorescence Cell Counter (Logos Biosystems, L20001). Single cell suspensions were loaded on a Chromium Single Cell 3′ Chip (10X Genomics) and were run

(7) An optimized workflow for single-cell transcriptomics and repertoire profiling of purified lymphocytes from clinical samplesScientific ReportsDecember 1, 2020Richa Hanamsagar, Timothy Reizis, Mathew Chamberlain, Robert Marcus, Frank O. Nestle, Emanuele de Rinaldis, Virginia Savovawere noted. LUNA-FL dual fluorescence cell counter LUNA-FL Dual Fluorescence Cell Counter (Cat # L20001, Logos Biosystems) was used as per manufacturer’s instructions. Briefly, cells were diluted with Acridine Orange/Propidium Iodide Stain

(8) Genome editing enables reverse genetics of multicellular development in the choanoflagellate Salpingoeca rosettaeLifeJune 4, 2020Booth DS, King N.https://doi.org/10.7554/eLife.56193 13 of 24 Research advance Developmental Biology Genetics and Genomics Anyang, KOR; Cat. No. L20001). After determining the cell concentration, the washed S. rosetta cells were diluted to a final concentration

(9) Increased O-GlcNAcylation of c-Myc Promotes Pre-B Cell ProliferationCellsJanuary 8, 2020Lee DH, Kwon NE, Lee WJ, Lee MS, Kim DJ, Kim JH, Park SK.trypan blue staining or a LUNA-FLTM Cell counter (Logos Biosystems, Anyang-si, Gyeonggi-do, Korea, L20001) with dual fluorescence staining, acridine orange (live cell detection), and propidium iodide (dead

(10) Furin-mediated intracellular self-assembly of olsalazine nanoparticles for enhanced magnetic resonance imaging and tumour therapyNature MaterialsDecember 1, 2019Yue Yuan, Jia Zhang, Xiaoliang Qi, Shuoguo Li, Guanshu Liu, Soumik Siddhanta, Ishan Barman, Xiaolei Song, Michael T. McMahon, Jeff W. M. BulteLogos Biosystems), using the â€œFluorescence Cell Countingâ€ mode on a Luna automated fluorescence cell counter (L20001, Logos Biosystems). Cells were either incubated Olsa-RVRR (pretreated with or without FI), olsalazine

(11) Single-cell transcriptomics reveals multi-step adaptations to endocrine therapyNature communicationsSeptember 2, 2019Hong SP, Chan TE, Lombardo Y, Corleone G, Rotmensz N, Bravaccini S, Rocca A, Pruneri G, McEwen KR, Coombes RC, et al.acridine orange/propidium iodide dye with LUNA-FL Dual Fluorescence Cell Counter (Logos Biosystems, L20001). Single-cell suspensions were loaded on a Chromium Single Cell 3′ Chip (10X Genomics), and were run

(12) Blocking nuclear export of HSPA8 after heat shock stress severely alters cell survivalScientific ReportsDecember 1, 2018Fengjuan Wang, Srinivasa Reddy Bonam, Nicolas Schall, Lauriane Kuhn, Philippe Hammann, Olivier Chaloin, Jean-Baptiste Madinier, Jean-Paul Briand, Nicolas Page, Sylviane Muller

(13) Transfection of choanoflagellates illuminates their cell biology and the ancestry of animal septinsMolecular Biology of the CellDecember 1, 2018David S. Booth, Heather Szmidt-Middleton, Nicole Kingformaldehyde was counted on a Luna-FL automated cell counter (Logos Biosystems, Anyang, Korea; Cat. No. L20001) and the remaining cells were diluted to a final concentration of 5 × 107 choanoflagellate cells/ml.

(14) Association of the Human Bocavirus With Tonsil Squamous Cell CarcinomasFrontiers in MicrobiologyOctober 16, 2018Merle Höpken, Isabel Förster, Steffen Maune, Michael Brockmann, Oliver Schildgen, Verena Schildgen

(15) Assay for galactose-deficient IgA1 enables mechanistic studies with primary cells from IgA nephropathy patientsBioTechniquesAugust 24, 2018Colin Reily, Dana V Rizk, Bruce A Julian, Jan Novak

(16) Canine mesenchymal stem cells from synovium have a higher chondrogenic potential than those from infrapatellar fat pad, adipose tissue, and bone marrowPLOS ONEAugust 23, 2018Akari Sasaki, Mitsuru Mizuno, Nobutake Ozeki, Hisako Katano, Koji Otabe, Kunikazu Tsuji, Hideyuki Koga, Manabu Mochizuki, Ichiro Sekiya

(17) A Single-Cell Transcriptome Atlas of the Aging Drosophila BrainCellAugust 9, 2018Davie K, Janssens J, Koldere D, De Waegeneer M, Pech U, Kreft Ł, Aibar S, Makhzami S, Christiaens V, Bravo González-Blas C, et al.

(18) HIV-1 targets L-selectin for adhesion and induces its shedding for viral releaseNature communicationsJuly 19, 2018Kononchik J, Ireland J, Zou Z, Segura J, Holzapfel G, Chastain A, Wang R, Spencer M, He B, Stutzman N, et al.

(19) Pan-cancer genome and transcriptome analyses of 1,699 pediatric leukemias and solid tumorsNatureFebruary 28, 2018Xiaotu Ma, Yu Liu, Yanling Liu, Ludmil B Alexandrov, Michael N. Edmonson, Charles Gawad, Xin Zhou, Yongjin Li, Michael C. Rusch, John Easton, Robert Huether, Veronica Gonzalez-Pena, Mark R. Wilkinson, Leandro C. Hermida, Sean Davis, Edgar Sioson, Stanley Pounds, Xueyuan Cao, Rhonda E. Ries, Zhaoming Wang, Xiang Chen, Li Dong, Sharon J. Diskin, Malcolm A. Smith, Jaime M. Guidry Auvil, Paul S. Meltzer, Ching C. Lau, Elizabeth J. Perlman, John M. Maris, Soheil Meshinchi, Stephen P. Hunger, Daniela S. Gerhard, Jinghui Zhang

(20) Strain-Specific Contribution of Eukaryotic Elongation Factor 1 Gamma to the Translation of Influenza A Virus ProteinsFrontiers in microbiologyJanuary 1, 2018Sammaibashi S, Yamayoshi S, Kawaoka Y.

Write a Review

(1)

12/19/2023Simple and Effective Tool for Quick Cell Counting Lana JagannathanWe use this cell counter for basic cell culture for a variety of cells and cell lines, including cancer cell lines and human donor-derived PBMCs.
(1)

10/26/2018I Can Finally Accurately Count My Primary Cells! Merel BotThe Luna FL works great! I used to use a brightfield cell counter based on Tryphan blue. The old counter gave me a lot of inaccurate results because of cell debris in my primary tissue culture samples... Read Review
(1)

9/14/2018Cell Count and Viability Using the LUNA-FLDr. Jenni LahtelaWe use LUNA-FL for counting and measuring cell viabilities prior submitting the cells to single cell RNA-seq analysis. LUNA-FL was chosen because of it's good capability to count cells and also ... Read Review
(1)

5/23/2016Accurate and Reliable Automated Cell counter Sivaraman NatarajanAutomated cell counter saves lot of time and accuracy in the counting is very important as part of the results/ treatment. We use this counter every day to count live & dead cells for our experiments ... Read Review

Improving the Accuracy of Automated Cell Counting
April 25, 2022
Step-by-step guide can help ensure accurate results. read more
CLARITY Enabling Volume Imaging of Intact Tissue
November 16, 2015
As scientists continue to study disease states in a variety of tissues, an understanding of the 3D structure of organs is providing insight into structure-function relationships that affect tissue functioning. Advanced ... read more

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LUNA-FL™ Dual Fluorescence Cell Counter 1

Luna-FL

LUNA-FL™ Dual Fluorescence Cell Counter from Logos Biosystems

Description

Specifications/Features

Logos Biosystems

Citations (55)

Reviews

Bench Tips

Improving the Accuracy of Automated Cell Counting

CLARITY Enabling Volume Imaging of Intact Tissue

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LUNA-FL™ Dual Fluorescence Cell Counter from Logos Biosystems

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