Cellometer Auto 2000 - for Primary Cell Viability using Dual Fluorescence from Nexcelom Bioscience

Since 2005, thousands of users, including the top ten pharmaceutical companies, have relied on Cellometer for analysis of cell lines and primary cells. The Auto 2000 Cell Viability Counter is specifically optimized for analysis of primary cells from heterogeneous samples, including peripheral blood, cord blood, and bone marrow. Researchers can utilize the Auto 2000 to assess viability after initial sample collection, following cell sorting, and after cryopreservation, even in samples containing platelets and red blood cells.

Analysis Applications

• PBMCs for Clinical Immunology
  
• Splenocytes for Vaccine Development
  
• Stem Cells for Regenerative Medicine
  
• Nucleated Cells for Transplantation
  
• Tumor Cell Suspensions for Oncology
  
• GFP Transfection Efficiency
  
• Cell Line Concentration & Viability

Auto 2000 Primary Cell Viability Method

The Auto 2000 utilizes the AO/PI staining method (Acridine Orange / Propidium Iodide) for identification of live and dead nucleated cells. The AO dye stains the DNA in all nucleated cells in order to differentiate nucleated cells from debris and unwanted cell types, such as platelets red blood cells. The PI dye stains the DNA in nucleated cells with compromised cell membranes, allowing for identification of dead nucleated cells. The ability to accurately analyze primary cells within samples containing varying amounts of red blood cells eliminates the need to lyse samples, simplifying the analysis process and reducing variability due to processing. With the traditional trypan blue method, non-nucleated cells can be incorrectly counted, yielding viability calculations that are too high.

Simple, Automated Analysis

The Auto 2000 features a user-friendly touch screen with pre-set assays for PBMCs, nucleated cells, stem cells, and other cell types. Simply pipette 20µl of stained sample into a disposable counting chamber, insert into the Auto 2000, select the appropriate assay, and click count. In less than 30 seconds, the Auto 2000 acquires and analyzes bright field and fluorescent images and displays live / dead cell counts, concentration, mean diameter, and percent viability.

The proprietary Cellometer software is optimized for accurate analysis of cells in clumps and irregular-shaped cells. User-specific assay protocols with customized counting parameters can be created for unique cell types. Automated analysis using tested mathematical algorithms eliminates the subjectivity and inter-operator variability associated with manual cell counting. The instrument can be set to auto-save results and images, eliminating transcription errors and creating a permanent record of the analysis results.

Instrument Features

• Bright field and dual-fluorescence imaging
  
• User-friendly touch screen
  
• Pre-set assays for primary cell analysis
  
• Small 20µl sample size
  
• Images & results in <30>
  
• Broad dynamic range: 105 to 107 cells / mL
  
• Accurate analysis of cells in clumps
  
• Fluorescent cell counting
  
• Auto-save option for images and data
  
• No washing. No maintenance.

Seeing is Believing!

We know the Cellometer Auto 2000 Cell Viability Counter is the best choice for simple, accurate primary cell analysis. We would like you to see for yourself. Evaluate an Auto 2000 in your laboratory for one full week free of charge or request an in-lab demonstration.