ImProm-II Reverse Transcription System from Promega

Product Specs
ItemImProm-II Reverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3800
Quantity100 reactions
Sample TypeTotal RNA, poly(A)+ mRNA or synthetic transcript RNA

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Selection of Molecules with Immunological Potential from Excretory and Secretory Products from the Nematode Haemonchus placei by Cell Proliferation and Gene Expression AssaysBIO-PROTOCOLJune 20, 2023Jocelyn Maza-Lopez, René Camas-Pereyra, María Eugenia López-Arellano, Carla O. Contreras-Ochoa(Promega, catalog number: M6101)ImProm-II Reverse Transcription System kit (Promega, catalog number: A3800)GoTaq®qPCR Master Mix 2× (Promega, catalog number: A6001)Custom RT2Profiler PCR array (Qiagen, catalog

(2) Transcriptional and metabolic programs promote the expansion of follicular helper T cells in lupus-prone miceiScienceMay 19, 2023Minghao Gong, Seung-Chul Choi, Yuk Pheel Park, Xueyang Zou, Ahmed S. Elshikha, Valerie A. Gerriets, Jeffrey C. Rathmell, Mansour Mohamazadeh, Laurence Morel,Miltenyi Biotec,130-104-453 BCA protein assay kit,Pierce,23225 Impromptu-II™ Reverse Transcription System,Promega,A3800 ,, Deposited data,Deposited data,Deposited data ,, Raw RNA-seq data,GEO,GSE 157648 Oher raw and

(3) DDM1-mediated gene body DNA methylation is associated with inducible activation of defense-related genes in ArabidopsisGenome BiologyMay 5, 2023Seungchul Lee, Jaemyung Choi, Jihwan Park, Chang Pyo Hong, Daeseok Choi, Soeun Han, Kyuha Choi, Tae-Young Roh, Daehee Hwang, Ildoo Hwangcat. no. AM1907). cDNA was produced with the ImProm-II Reverse Transcription System (Promega, cat. No. A3800). SYBR Premix Ex Taq (Takara, cat. No. RR420A) and LightCycler 2.0 (Roche, cat. No. 03531414001), StepOnePlus™ Real-Time PCR System

(4) The Flavonoid Cyanidin Shows Immunomodulatory and Broad-Spectrum Antiviral Properties, Including SARS-CoV-2VirusesApril 18, 2023Josefina Vicente, Martina Benedetti, Paula Martelliti, Luciana Vázquez, María Virginia Gentilini, Freddy Armando Peñaranda Figueredo, Mercedes Soledad Nabaes Jodar, Mariana Viegas, Andrea Alejandra Barquero, Carlos Alberto Buenomouse lungs. Then, reverse transcription was performed using ImProm-II™ Reverse Transcription System A3800 (Promega, Madison, WI, USA), following the manufacturer’s instructions. Detection was performed using

(5) Medicago truncatula quantitative resistance to a new strain of Verticillium alfalfae from Iran revealed by a genome-wide association studyFrontiers in Plant ScienceApril 14, 2023Amir Hossein Fartash, Cécile Ben, Mélanie Mazurier, Asa Ebrahimi, Mojtaba Ghalandar, Laurent Gentzbittel, Martina Rickauersynthesized from one microgram of pure RNA with the ImProm-II ™ Reverse Transcription System kit (Promega, A3800) using Oligo (dT)15 primer following the manufacturer’s instructions. qRT-PCR was performed with the

(6) Physiologic Response Evaluation of Human Foetal Osteoblast Cells within Engineered 3D-Printed Polylactic Acid ScaffoldsBiology (Basel)March 10, 2023Maria Giovanna Rizzo, Nicoletta Palermo, Paola Alibrandi, Emanuele Luigi Sciuto, Costantino Del Gaudio, Vincenzo Filardi, Barbara Fazio, Antonella Caccamo, Salvatore Oddo, Giovanna Calabrese, et al.cDNA was synthesised from an equal amount of total RNA using ImProm-II Reverse Transcription System (A3800, Promega, Milan, Italy). Quantitative real-time PCR (qRT-PCR) was performed using a 7500 Fast Real-Time

(7) Platelet pannexin-1 channels modulate neutrophil activation and migration but not the progression of abdominal aortic aneurysmFrontiers in Molecular BiosciencesMarch 6, 2023Lisa Maria Metz, Tobias Feige, Larissa de Biasi, Agnes Ehrenberg, Joscha Mulorz, Laura Mara Toska, Friedrich Reusswig, Christine Quast, Norbert Gerdes, Malte Kelm, et al.min. Afterwards, cDNA synthesis was conducted by using the ImProm-IITM Reverse Transcription System (#A3800, Promega), following the manufacturer’s instructions. For cDNA synthesis, a total amount of 100 ng RNA

(8) Protective Effect of Vitamin D against Hepatic Molecular Apoptosis Caused by a High-Fat Diet in RatsCurrent Issues in Molecular BiologyJanuary 5, 2023Huda F. Alshaibi, Sherin Bakhashab, Asma Almuhammadi, Yusuf S. Althobaiti, Mohammed A. Baghdadi, Khadeejah AlsolamiμL using a reverse kit (ImProm-IITm Reverse Transcription System, Promega, Madison, WI, USA, cat no. A3800), according to the manufacturer’s instructions. The reaction was conducted on a thermal cycler with

(9) Therapeutic effect of combination vitamin D3 and siponimod on remyelination and modulate microglia activation in cuprizone mouse model of multiple sclerosisFrontiers in Behavioral NeuroscienceJanuary 5, 2023Kholoud M. Al-Otaibi, Badrah S. Alghamdi, Maryam A. Al-Ghamdi, Rasha A. Mansouri, Ghulam Md Ashraf, Ulfat M. Omarcomplementary DNA (cDNA) was synthesized from RNA using ImProm-IITM Reverse Transcription System (Promega; A3800) according to the manufacturer’s protocol. qRT-PCR was performed using BioFACTTM 2X Real-Time PCR Master

(10) Intermittent compressive force regulates dentin matrix protein 1 expression in human periodontal ligament stem cellsJournal of dental sciencesJanuary 1, 2023Manokawinchoke J, Chareonvit S, Trachoo V, Limraksasin P, Egusa H, Osathanon T.was converted to complementary DNA (cDNA) using Reverse Transcription System (ImProm-II™, cat. No. A3800, Promega, Madison, WI, USA). The cDNA was employed for quantitative real-time polymerase chain reaction

(11) Polysaccharide of Atractylodes macrocephala Koidz alleviate lipopolysaccharide-stimulated liver inflammation injury of goslings through miR-223/NLRP3 axisPoultry ScienceJanuary 1, 2023Feiyue Chen, Bingxin Li, Wanyan Li, Wenbin Chen, Yunmao Huang, Yunbo Tian, Baohe Yang, Mingfeng Yuan, Danning Xu, Nan Caofor miR-223 reverse transcription according to instructions of ImPro-Ⅱ Reverse Transcription System (A3800, Promega, WI). After that, quantitative real-time polymerase chain reaction assays were conducted on

(12) Effects of ethylenediaminetetraacetic acid on stem cells from the apical papilla: In vitro studyJournal of Dental SciencesJanuary 1, 2023Suphalak Phothichailert, Benya Sangwisutsai, Dhitinant Rattanakosol, Nutcha Teerapongpaibul, Sirawut Hiran-us, Nunthawan Nowwarote, Thanaphum OsathanonKorea) and converted to cDNA using reverse transcriptase Improm-II ™ RT system kit (ImProm-II™, cat. No. A3800, Promega, Madison, WI, USA). A real-time polymerase chain reaction was performed in a real-time PCR system

(13) Single-cell transcriptomics identifies Mcl-1 as a target for senolytic therapy in cancerNature CommunicationsDecember 1, 2022Martina Troiani et al.15596026), according to the manufacturer’s instructions. cDNA was obtained using ImPROM II kit (Promega, A3800) according to the manufacturer’s instructions. RT-qPCR was performed using Gotaq® qPCR Master Mix, Promega®

(14) Expression profile of synaptic vesicle glycoprotein 2A, B, and C paralogues in temporal neocortex tissue from patients with temporal lobe epilepsy (TLE)Molecular BrainDecember 1, 2022Burcu A. Pazarlar, Sanjay S. Aripaka, Viktor Petukhov, Lars Pinborg, Konstantin Khodosevich, Jens D. MikkelsenRNA was reverse transcripted to single-stranded cDNA by using Promega reverse transcription system (A3800) and the BioRad ICycler Thermal Cycler Instrument. cDNA was synthesized in a final volume of 20 µL reaction

(15) Pentacyclic triterpenes modulate farnesoid X receptor expression in colonic epithelial cells: Implications for colonic secretory functionThe Journal of biological chemistryNovember 1, 2022Fallon CM, Smyth JS, Quach A, Lajczak-McGinley N, O'Toole A, Barrett KE, Sheridan H, Keely SJ.performed on purified RNA (0.75 μg) using the Improm-II Reverse Transcription Kit (Promega; Catalog # A3800), as per manufacturer’s protocol. The reverse transcription program was run on a PCR Thermal Cycler

(16) Protein synthesis inhibition and loss of homeostatic functions in astrocytes from an Alzheimerâ€™s disease mouse model: a role for ER-mitochondria interactionCell Death & DiseaseOctober 18, 2022Laura Tapella, Giulia Dematteis, Marianna Moro, Beatrice Pistolato, Elisa Tonelli, Virginia Vita Vanella, Daniele Giustina, Aleida La Forgia, Elena Restelli, Elettra Barberis, et al.First strand of cDNA was synthesized from 0.5–1 µg of total RNA using Im-Prom-II system (Promega, Cat. A3800). Real-Time PCR was performed using iTaq qPCR master mix according to manufacturer’s instructions (Bio-Rad,

(17) The Relationship between DUGBE Virus Infection and Autophagy in Epithelial CellsVirusesOctober 11, 2022Marie Moroso, Aurore RoziÃ¨res, Pauline Verlhac, Florence Komurian-Pradel, Olivier Ferraris, Christophe N. Peyrefitte, Glaucia Paranhos-BaccalÃ , Christophe Viret, Mathias Faurecarried out using the ImPromII Reverse Transcription kit (Promega, Charbonnières les Bains, France, A3800), iTaq universal SYBR Green Supermix (Bio-Rad, 170-8882) and Bio-Rad CFX96. 2.8. Fluorescence Microscopy DUGV-infected

(18) Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modellingEMBO Molecular MedicineOctober 10, 2022SungWoo Choi, Giulia Ferrari, Louise A Moyle, Kirsty Mackinlay, Naira Naouar, Salma Jalal, Sara Benedetti, Christine Wells, Francesco Muntoni, Francesco Saverio TedescoRetro‐transcription to cDNA was performed with the ImProm‐IITM Reverse Transcription System kit (Promega, A3800) following manufacturer's instructions; a minimum of 50 ng of RNA per reaction was used. qRT‐PCRs were

(19) Betaine promotes osteogenic differentiation in immortalized human dental pulp-derived cellsBDJ OpenOctober 7, 2022Chatvadee Kornsuthisopon, Dusit Nantanapiboon, Sunisa Rochanavibhata, Nunthawan Nowwarote, Worachat Namangkalakul, Thanaphum Osathanonsubsequently converted to complementary DNA using ImProm-II Reverse Transcription System (Cat. no. A3800, Promega, USA) (n = 6). RT-qPCR was performed using the CFX Connect Real-Time PCR machine (Bio-Rad,

(20) Diabetic Covid-19 severity: Impaired glucose tolerance and pathologic bone lossBiochemical and Biophysical Research CommunicationsSeptember 10, 2022Jyotirmaya Behera, Jessica Ison, Michael J. Voor, Suresh C. Tyagi, Neetu Tyagiμg) following the instruction of the ImProm-II™ Reverse Transcription System manufacturer's protocol (A3800, Promega). Primers (forward and reverse) against target genes of interest were used for real-time PCR

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ImProm-II™ Reverse Transcription System from Promega
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