The ZOOM® IPGRunner™ System is the first system to offer oil-free, trouble-free 2D electrophoresis in a mini-gel format. First dimension separation, isoelectric focusing (IEF), is complete in less than three hours. The mini-gel design of the system is easy to handle, eliminates mineral oil overlays, and allows processing of up to 12 samples at once for high-throughput usage. The ZOOM® IPGRunner™ System consists of three main components:
- The ZOOM® IPGRunner™ Mini-Cell (Figure 1) includes a high-voltage electrophoresis core and lid assembly that fits a mini-cell chamber identical to the XCell SureLock™ mini-cell. The mini-cell holds two ZOOM® IPGRunner™ Cassettes.
- The ZOOM® IPGRunner™ Cassettes (Figure 2) are provided ready to use and self-contained, eliminating oil overlays for convenient sample rehydration and IEF. Each cassette holds up to six ZOOM® Strips.
- The ZOOM® Strips are 7 cm long and contain a thin layer of polyacrylamide gel that includes a fixed pH gradient. Each ZOOM® Strip is clearly labeled with a unique identifying number, pH range, and orientation marks (Figure 3). ZOOM® Strips are supplied attached to a tri-fold card (Figure 4) for easy access and removal.
Quick and Simple Set-up ProcedureFigure 5 demonstrates how easy and fast it is to load samples and set up the ZOOM® IPGRunner™ System for IEF. Once IEF is complete, the ZOOM® IPGRunner™ Mini-Cell can be used with NuPAGE® and Novex® ZOOM® gels to complete SDS-PAGE analysis in 40 minutes.