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BspHI
BspHI
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BspHI
BspHI from New England Biolabs
Be the first to write a review!
Citations:
(7)
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New England Biolabs for
BspHI
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Description
BspH I
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
BspHI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0517S
Quantity
500 units
Concentration
10,000 units/ml
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
T/CATGA
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (7)
(1)
Alternative polyadenylation alters protein dosage by switching between intronic and 3′UTR sites
Science Advances
February 17, 2023
Nicola de Prisco, Caitlin Ford, Nathan D. Elrod, Winston Lee, Lauren C. Tang, Kai-Lieh Huang, Ai Lin, Ping Ji, Venkata S. Jonnakuti, Lia Boyle,
et al.
microliters of the 666-bp
PCR product
was digested with 0.3 μl of Bsph I (
New England Biolabs
, catalog no.
R0517
). The C>T mutation creates a Bsph I restriction site; the
PCR product
from wild-type
zebrafish
will
(2)
Plasmid-DNA Delivery by Covalently Functionalized PEI-SPIONs as a Potential 'Magnetofection' Agent
Molecules
November 1, 2022
René Stein, Felix Pfister, Bernhard Friedrich, Pascal-Raphael Blersch, Harald Unterweger, Anton Arkhypov, Andriy Mokhir, Mikhail Kolot, Christoph Alexiou, Rainer Tietze
using the
BspHI
enzyme following the manufacturers’ instructions (
New England BioLabs
, Product No.
R0517S
). A volume of 2 µL of 6xPPP loading dye were mixed with 10 µL of each sample. The samples consisted
(3)
pRB-Depleted Pluripotent Stem Cell Retinal Organoids Recapitulate Cell State Transitions of Retinoblastoma Development and Suggest an Important Role for pRB in Retinal Cell Differentiation
Stem Cells Translational Medicine
April 29, 2022
Agata Rozanska et al.
grown in 24-well plates to be assessed for
gene editing
with restriction
enzyme assay
(
BspHI
,
NEB
,
R0517L
). Selected
clones
were sequenced to confirm the introduction of the mutation to the wild-type allele
(4)
Efficient genome editing of an extreme thermophile, Thermus thermophilus, using a thermostable Cas9 variant
Scientific Reports
December 1, 2021
Bjorn Thor Adalsteinsson, Thordis Kristjansdottir, William Merre, Alexandra Helleux, Julia Dusaucy, Mathilde Tourigny, Olafur Fridjonsson, Gudmundur Oli Hreggvidsson
µg/ml) and plasmids miniprepped (NEB, T1010). The miniprepped plasmids were restricted with
BspHI
(
NEB
,
R0517
) and separated on agarose gel to confirm presence of inserts. Plasmids pTTCC_purA and pTTCC_crtI were
(5)
Transposon insertion profiling by sequencing (TIPseq) for mapping LINE-1 insertions in the human genome
Mobile DNA
December 1, 2019
Jared P. Steranka, Zuojian Tang, Mark Grivainis, Cheng Ran Lisa Huang, Lindsay M. Payer, Fernanda O. R. Rego, Thiago Luiz Araujo Miller, Pedro A. F. Galante, Sitharam Ramaswami, Adriana Heguy,
et al.
no. BP2818500) (CAUTION Ethanol is highly flammable)
AseI
(
NEB
, cat. no.
R0526S
)
BspHI
(
NEB
, cat. no.
R0517S
)
BstYI
(
NEB
, cat. no.
R0523S
)
HindIII
(
NEB
, cat. no.
R0104S
)
NcoI
(
NEB
, cat. no.
R0193S
)
PstI
(
NEB
, cat.
(6)
Engineered dCas9 with reduced toxicity in bacteria: implications for genetic circuit design
Nucleic Acids Research
October 5, 2018
Shuyi Zhang, Christopher A Voigt
transformation, these plasmids were re-purified and digested with restriction enzyme
BsphI
(
New England Biolabs
, #
R0517S
) to make sure they have the expected sizes and thus rule out the possibility of unwanted homologous
(7)
A simple PCR-based genotyping method for M105I mutation of alpha-SNAP enhances the study of early pathological changes in hyh phenotype
Molecular and Cellular Probes
July 7, 2009
Luis Federico Bátiz, Ruth Roales-Buján, Luis M. Rodríguez-Pérez, Isabel M. Matas, Patricia Páez, María Roque, Antonio J. Jiménez, Cayo Ramos, José Manuel Pérez-Fígares
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BspHI from New England Biolabs
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