Unlike most other kits, all of the components of the Versagene kit can be stored on the shelf. Also, all liquid components, of which there are only four, come ready to use...
After receiving a free sample of Eppendorf HotMasterMix, I did a complete 96 well plate reaction and found that I had greater than 90% success. Agarose gel electrophoresis confirmed that the amplification products were the correct size (based on the DNA ladder) and there were no non-specific bands...
I have used plasmid mini-prep kits from many different companies. But recently I began using the Brinkmann Eppendorf FastPlasmid Mini kit...
Hot Start DNA polymerases have become very popular because of their convenience
and their ability to reduce nonspecific amplification. Typical Hot Start
enzymes utilize chemical or antibody mediated inhibition, which impart
disadvantages such as a long initial denaturing step or excessive protein
contamination. In contrast, Eppendorf’s HotMaster uses an innovative
temperature-dependent inhibitor to achieve Hot Start activity.
The classic RNA isolation procedures are based on a mono-phasic solution
of phenol and guanidinium isothiocyanate solutions, followed by organic
extraction and alcohol precipitation of the RNA.1 TRIzol is the commercial
version of this popular single-step method of total RNA isolation. This
highly reliable technique performs well with both small and large quantities
of tissues or cultured cells and allows simultaneous processing of a large
number of samples.
Phenol extraction of nucleic acids has always been an unpleasant element of any molecular biology protocol. The Phase Lock Gel (PLG) from Eppendorf delivers an elegant solution to this inconvenience...
The average miniprep requires 15-40 minutes for a single sample and about an hour when following manufacturers instructions (even Eppendorf's own older version of this product required 25 minutes). The new PerfectPrep system can process a sample in 5 minutes and 10 samples in under 20 minutes.