pENTR/SD/D-TOPO Cloning Kit, with One Shot TOP10 Chemically Competent E. coli from Life Technologies

pENTR/SD/D-TOPO Cloning Kit, with One Shot TOP10 Chemically Competent E. coli from Life Technologies

Description

The pENTR /SD/D-TOPO Cloning Kits utilize a highly efficient, 5-minute cloning strategy ( TOPO Cloning ) to directionally clone a blunt-end PCR product into a vector for entry into the Gateway System or the MultiSite Gateway System. Blunt-end PCR products clone directionally into the pENTR /SD/D-TOPO entry vector at greater than 90% efficiency, and include an upstream Shine-Dalgarno sequence for expression in prokaryotes. The kit comes with everything necessary to clone and select your PCR amplified gene of interest: Gateway System Ready - Rapidly shuttle cloned genes between multiple vector systems Fast and Easy - Go from PCR to Gateway Entry clone in just 3 steps and as little as ~5 minutes hands on time Efficient - Achieve over 90% clones with correct insert in the right direction Proven - Reliable performance for over a decade <di

Specifications

  • Detailed Specifications
  • ItempENTR/SD/D-TOPO Cloning Kit, with One Shot TOP10 Chemically Competent E. coli
  • CompanyLife Technologies
  • Price Pricing InfoGet Quote
  • Catalog NumberK242020
  • Quantity20 reactions
  • Vector NamepENTR/SD/D-TOPO
  • ApplicationsCloning,Entry Vectors & Kits,Gateway Cloning,pENTR Vectors & Kits
  • Resistancekanamycin
  • HostBacteria

Specifications/Features

Features:
Blunt Cloning Kits

Vector Size:
2580 bp

Kit Contents:
with One Shot® TOP10 Chemically Competent E. coli with One Shot® Mach1™-T1R Chemically Competent E. coli

Life Technologies
Life Technologies
5791 Van Allen Way
Carlsbad, CA 92008
United States
Phone: (760) 603-7200

Citations