Perform apoptosis analysis based on changes in mitochondrial membrane potential with only 20µl of sample. See how image-based analysis simplifies cell-based assays. In just a few clicks, acquire cell images and obtain colorized scatter plots and data tables. Easily compare control and experimental samples using a simple, accurate, image-based method.
Accurate cell concentration and viability calculations are critical for successful cell-based assays and flow cytometry analysis. The accuracy and reproducibility of the Cellometer Auto T4 Cell Counter have been demonstrated in thousands of labs, including the top ten pharmaceutical companies.
Since 2005, thousands of users, including the top ten pharmaceutical companies, have relied on Cellometer for analysis of cell lines and primary cells. The Auto 2000 Cell Viability Counter is specifically optimized for analysis of primary cells from heterogeneous samples, including peripheral blood, cord blood, and bone marrow. Researchers can utilize the Auto 2000 to assess viability after initial sample collection, following cell sorting, and after cryopreservation, even in samples containing platelets and red blood cells.
Analysis Applications
PBMCs for Clinical Immunology
Splenocytes for Vaccine Development
Stem Cells for Regenerative Medicine
Nucleated Cells for Transplantation
Tumor Cell Suspensions for Oncology
GFP Transfection Efficiency
Cell Line Concentration & Viability
Auto 2000 Primary Cell Viability Method The Auto 2000 utilizes the AO/PI staining method (Acridine Orange / Propidium Iodide) for identification of live and dead nucleated cells. The
Perform two-color fluorescence to count live and dead primary cells from peripheral blood, cord blood, and bone marrow. Accurately measure PBMCs, stem cells, splenocytes, tumor lysates, and nucleated cells in samples containing debris and red blood cells in 30 seconds using sample-specific assays and a simple touch screen.
Simplify apoptosis experiments with image-based analysis. Colorized data plots easily identify apoptotic and necrotic cells. Manually adjust gating with instant updates to data tables. View brightfield and fluorescent images for confirmation of results. Easily switch between users and experiments with no set-up, washing, or potential clogging.
Perform cell cycle analysis with only 20µl of sample. See how image-based analysis simplifies cell-based assays. In just a few clicks, acquire cell images and obtain comprehensive plots and tables displaying cell population by phase. Easily switch between users and experiments with no set-up, washing, or potential clogging.
Automatically generate yeast concentration and viability data from a single sample. See how brightfield and fluorescent imaging simplifies and enhances yeast fermentation monitoring with 1-click image capture and calculation of yeast concentration, % viability, and cell size. Save time and improve data quality and reproducibility vs. manual counting.
The Cellometer® Vision Cell Analyzer incorporates
image based cell counting and fluorescence
detection in a compact and easy-to-use instrument.
With dual fluorescence detection capabilities,
Cellometer Vision is an ideal solution for many
complex cell population characterization assays,
such as reliable counting and viability determination
of primary hepatocytes.
A Cellometer assay principal illustration of a dual-fluorescent FDA/PI assay for cell concentration and viability.
Unlike manual counting, Cellometer measures both individual and mean cell sizes, and can generate size distribution histograms for all counted cells. Multiple histograms can be overlaid for determining changes in cell sizes over time.
A Cellometer assay principal illustration of a dual-fluorescent AO/PI assay for cell concentration and viability. This assay is most suitable for primary cell samples which contain debris and unwanted non-nucleated cell types.
A Cellometer assay principal illustration of a dual-fluorescent AO/EB assay for cell concentration and viability. This assay is most suitable for primary cell samples such as white blood cells, splenocytes and lymphocytes which contain debris and unwanted non nucleated cell types such as red blood cells.
A Cellometer assay principal illustration of a dual-fluorescent CalceinAM/PI assay for cell concentration and viability Calcein-AM penetrates the cell membrane of both living and dead cells. Once inside a living cell, Calcein-AM is hydrolyzed by cellular esterases into the cell membrane-impermeable Calcein. PI is only permeable to dead cells.
Cellometer Vision is a line of simple to use, automated cell analyzers with diverse functions. It combines bright field microscopy and multi-channel fluorescence images to generate cell count and fluorescence data. This video demonstration features the Cellometer Vision to measure live cell concentration and viability of human PBMC.
Cellometer Auto X4 is a line of simple to use, automated cell counters with fluorescence detection. It combines bright field microscopy with fluorescence to identify selected cells. This video demonstration features the Cellometer Auto X4 to measure algae cell concentration from a pond water sample.