RNAstable™ is a novel RNA preservation product developed to protect RNA samples from degradation during storage
or shipment at ambient temperatures. RNAstable is formulated by combining extremophile biology (long-term survival
in extremely dry environments in an arrested life form) and synthetic chemistry. Anhydrobiosis (meaning “life without
water”) allows some organisms to protect their DNA, RNA, proteins, membranes and cellular systems for survival; they
are revived by simple rehydration. RNAstable was designed to mimic these unique characteristics to stabilize RNA at
ambient temperatures.
RNAstable is a novel RNA stabilization product developed to protect RNA samples from degradation dry at room temperature. RNAstable is based on the natural principles of anhydrobiosis (meaning “life without water”), a biological mechanism employed by some multicellular organisms that enables their survival while dry for up to 120 years. Anhydrobiotic organisms such as tardigrades and brine shrimp protect their DNA, RNA, proteins, membranes and cellular systems for survival and can be revived by simple re-hydration. By exploiting these unique characteristics, RNAstable was designed to stabilize RNA dry at ambient temperatures. This innovative technology offers tremendous cost and energy savings as an easy-to-use alternative to conventional storage at -20°C or -80°C and eliminates expensive shipments on dry ice. Studies demonstrate that RNA is stabilized for up to 6 months at room temperature and even at elevated temperatures (50°C), making RNAstable an ideal transport media for precious, labile RNA samples. The data presented demonstrates that RNA stabilized in RNAstable maintains its integrity during long-term dry storage at room temperature and even at elevated temperatures. Samples recovered by simple re-hydration can be used directly in downstream applications with no inhibition or loss due to degradation.
RNAstable™ is a novel RNA preservation product designed to protect RNA samples from degradation during storage or shipment at
ambient temperatures. RNAstable is formulated based on the natural principles of a biological mechanism called anhydrobiosis
(meaning “life without water”). Anhydrobiotic organisms protect their cellular contents and structures for survival while in a dried
state for over 100 years and can be revived with simple rehydration. RNAstable was designed to mimic these unique
characteristics through synthetic chemistry to stabilize RNA dry at ambient temperatures. The data presented demonstrate
successful amplification with quantitative RT-PCR (qRT-PCR), using a RNA template that was stored dry in RNAstable for 4 weeks
at room temperature. Samples recovered by rehydration were used directly in reactions without further purification and exhibited no
inhibition or loss of activity. This innovative technology offers significant cost and energy savings as an easy-to-use alternative to
conventional cold sample storage and shipment.
Polymerase chain reaction (PCR) is one of the most performed molecular techniques not only in the academic and industrial
research world but also in the forensics, veterinary and health industries. In PCR, DNA is exponentially amplified using Taq DNA
polymerase from Thermus aquaticus, a bacterium found in hot springs. PCRboost™ was designed to enhance the amplification of
DNA without the need for time consuming optimizations. PCRboost™ allows for a decrease in the number of PCR cycles and use of
Taq DNA polymerase, resulting in significant time and money savings.