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PCR Master Mixes (High Fidelity)
Q5 High-Fidelity 2X Master Mix
Supplier Images
Q5 High-Fidelity 2X Master Mix
Q5 High-Fidelity 2X Master Mix from New England Biolabs
(1)
Write a review
Citations:
(284)
Supplier Page
Supplier Page from
New England Biolabs for
Q5 High-Fidelity 2X Master Mix
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Q5 High-Fidelity 2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0492L
Quantity
500 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (284)
(1)
Noncoding function of super enhancer derived mRNA in modulating neighboring gene expression and TAD interaction
bioRxiv
December 5, 2023
Bingning Xie, Ann Dean
catalog no. 76081–766) and
PCR amplification
with
Q5® High-Fidelity 2X Master Mix
(
NEB
, catalog no.
M0492S
).
PCR
products were confirmed by
Sanger sequencing
(Genewiz) dCasRX sgRNAs were designed by using
(2)
Protocol for selecting single human pluripotent stem cells using a modified micropipetter
STAR Protocols
December 1, 2023
Sandra P. Mojica-Perez, Kyle Stokes, Daniel C. Jaklic, Sheetal Jahagirdar, Michael Uhler, Jack M. Parent, Wei Niu
commercial
assays
,Critical commercial
assays
,, Q5 High-Fidelity 2× Master Mix,
New England Biolabs
,Cat#
M0492S
QIAquick PCR Purification Kit,
QIAGEN
,Cat#
28104
DNeasy Blood & Tissue Kit,
QIAGEN
,Cat#
69504
Infinium
(3)
Oxygenolytic sulfoquinovose degradation by an iron-dependent alkanesulfonate dioxygenase
iScience
October 20, 2023
Zonghua Ye, Yifeng Wei, Li Jiang, Yan Zhang
assays
,Critical commercial
assays
,Critical commercial
assays
,, Q5 High-Fidelity 2× Master Mix,
NEB
,Cat#
M0492L
GoScript™ Reverse Transcription System
,
Promega
,Cat#
A5000
GoTaq® qPCR Master Mix
,
Promega
,Cat#
A6001
RNAprep
(4)
Efficient Large DNA Fragment Knock-in by Long dsDNA with 3′-Overhangs Mediated CRISPR Knock-in (LOCK) in Mammalian Cells
BIO-PROTOCOL
October 20, 2023
Wenjie Han, Haojun Liang, Jianqiang Bao
(
Lonza
, catalog number:
VCA-1003
)Q5 High-Fidelity 2× Master Mix (
New England Biolabs
, catalog number:
M0492L
)50× TAE buffer (Sangon Biotech, catalog number: B548101)6× Gel Loading Dye Purple (
New England Biolabs
,
(5)
Unannotated Open Reading Frame in Saccharomyces cerevisiae Encodes Protein Localizing to the Endoplasmic Reticulum
MicroPubl Biol
October 20, 2023
Scott Chang, Matthew Joyson, Anna Kelly, Lucas Tang, John Iannotta, April Rich, Nelson Castilho Coelho, Anne-Ruxandra Carvunis
study Reagents,Reagents,Reagents,Reagents,Reagents Name,Final Concentration,Reference,Reference,Company 2x Q5 Master Mix,1x,
M0492L
,
M0492L
,
NEB
BioLabs LiAc,10mM,L4158,L4158,Millipore Sigma PEG,37%,P4338,P4338,Millipore Sigma ssDNA,2mg/ml,15632-011,15632-011,Invitrogen Hygromycin
(6)
The Endolysosomal Transporter DMT1 is Required for Morphine Regulation of Neuronal Ferritin Heavy Chain
Journal of Neuroimmune Pharmacology
September 4, 2023
Elena Irollo, Bradley Nash, Jared Luchetta, Renato Brandimarti, Olimpia Meucci
NEB
#E5520, New England Biolabs). Fragments were amplified with
Q5 High-Fidelity 2X Master Mix
(
NEB
#
M0492S
,
New England Biolabs
). The pLKO.1 vector used to introduce shRNAs targeting DMT1 was a gift from Bob
(7)
Single-cell massively-parallel multiplexed microbial sequencing (M3-seq) identifies rare bacterial populations and profiles phage infection
Nature Microbiology
August 31, 2023
Bruce Wang, Aaron E. Lin, Jiayi Yuan, Katherine E. Novak, Matthias D. Koch, Ned S. Wingreen, Britt Adamson, Zemer Gitai
double-stranded library was amplified using
PCR
by adding 30 μl of 2× Q5 High Fidelity master mix (
NEB
M0492L
), 0.4 μl 100 µM oDS028 and 0.4 μl 100 µM oBW170. We amplified the library using the following protocol:
(8)
Fucosylated glycoproteins and fucosylated glycolipids play opposing roles in cholera intoxication
bioRxiv
August 3, 2023
Atossa C. Ghorashi, Andrew Boucher, Stephanie A. Archer-Hartmann, Nathan B. Murray, Rohit Sai Reddy Konada, Xunzhi Zhang, Chao Xing, Parastoo Azadi, Ulf Yrlid, Jennifer J. Kohler
amino acid sequence.
Amplification
was done using the
Q5 High-Fidelity 2X Master Mix
(
NEB
, catalog no.
M0492S
) according to manufacturer’s instructions. Amplified ORFs were then purified using the MinElute PCR
(9)
Characterisation of a Live-Attenuated Rabies Virus Expressing a Secreted scFv for the Treatment of Rabies
Viruses
July 31, 2023
Samuel P. Smith, Rebecca Shipley, Pascal Drake, Anthony R. Fooks, Julian Ma, Ashley C. Banyard
08-115
)-coated T75 flasks. 2.2. Molecular Biology
Techniques
PCR
was undertaken using NEB Q5® DNA Polymerase (
M0492L
) according to the manufacturer’s instructions and products isolated using either
NEB
Monarch® DNA Gel Extraction Kit
(10)
Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ
eLife
July 28, 2023
Zhi Yang Tan, Shujun Cai, Alex J Noble, Jon K Chen, Jian Shi, Lu Gan
at the restriction site5’-GATATC-3’ Other,Q5 High-Fidelity 2× Master Mix,
New England BioLabs
,Cat#
M0492
,"Contains Q5 DNA Polymerase, deoxynucleotides and Mg2+ in buffer, for
PCR amplification
" Other,Salmon
(11)
Targeting AAV vectors to the central nervous system by engineering capsid–receptor interactions that enable crossing of the blood–brain barrier
PLOS Biology
July 19, 2023
Qin Huang, Albert T. Chen, Ken Y. Chan, Hikari Sorensen, Andrew J. Barry, Bahar Azari, Qingxia Zheng, Thomas Beddow, Binhui Zhao, Isabelle G. Tobey,
et al.
(CACTCATCGACCAATACTTGTACTATCTCT) as a forward primer in a
PCR
reaction using
Q5 High-Fidelity 2X Master Mix
(
NEB
#
M0492S
) following the manufacturer’s protocol for 30 cycles with 10 ng pUC57-wtAAV9-X/A plasmid. To assemble
(12)
Pleiotropy of autism-associated chromatin regulators
Development (Cambridge, England)
July 15, 2023
Lasser M, Sun N, Xu Y, Wang S, Drake S, Law K, Gonzalez S, Wang B, Drury V, Castillo O,
et al
.
individuals with ASD were created by
site-directed mutagenesis
using Q5 High Fidelity Master Mix (
NEB
M0492L
),
T4 polynucleotide kinase
(
NEB
M0201L
) and
T4 DNA ligase
(
NEB
M0202L
). Variants were selected by presence
(13)
mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine
Bio-protocol
July 5, 2023
Gao X, Li Y, Nie G, Zhao X.
(
New England Biolabs
, catalog number:
E2060S
)Q5 high-fidelity 2× master mix (
New England Biolabs
, catalog number:
M0492S
)Tris-HCl (1 M, pH 8.0) (Solarbio, catalog number: T1150)MgCl2 (1 M) (Beyotime, catalog number: ST269)Diethylpyrocarbonate
(14)
Biochemical and structural characterization of the first-discovered metazoan DNA cytosine-N4 methyltransferase from the Bdelloid rotifer Adineta vaga
Journal of Biological Chemistry
July 5, 2023
Jujun Zhou, John R. Horton, Gundeep Kaur, Qin Chen, Xuwen Li, Fabian Mendoza, Tao Wu, Robert M. Blumenthal, Xing Zhang, Xiaodong Cheng
Multiplex
Oligos for Illumina (
NEB
,
E7335S
/
E7500S
/
E7710S
/
E7730S
) and
Q5 High-Fidelity 2X Master Mix
(
NEB
,
M0492S
) (cycle number was determined by
qPCR
with
PowerUP SYBR Green Master Mix
using R1-22nt (5′-CTA CAC GAC
(15)
Assessment of enzymatically synthesized DNA for gene assembly
Frontiers in Bioengineering and Biotechnology
July 5, 2023
Brooke L. Simmons, Nathan D. McDonald, Natalie G. Robinett
incubated at 50°C/30 min. Next, 5 µL of this mixture was added to 12.5 µL of ×2 Q5 Master Mix (
NEB
, #
M0492S
), 2.5 µL each end oligo, and H2O to reach a final concentration of 25 µL. The
PCR
cycles were run as
(16)
Aclarubicin stimulates RNA polymerase II elongation at closely spaced divergent promoters
Science Advances
June 16, 2023
Matthew Wooten, Brittany Takushi, Kami Ahmad, Steven Henikoff
and a uniquely barcoded i7 primer and amplified with
NEB
Q5 high-fidelity 2× master mix (catalog no.
M0492S
). The libraries were purified with 1.1× volume of Sera-Mag carboxylate-modified magnetic beads and subjected
(17)
Modularity and diversity of target selectors in Tn7 transposons
Molecular Cell
June 15, 2023
Guilhem Faure, Makoto Saito, Sean Benler, Iris Peng, Yuri I. Wolf, Jonathan Strecker, Han Altae-Tran, Edwin Neumann, David Li, Kira S. Makarova,
et al.
NEBNext® High-Fidelity 2X PCR Master Mix
,
New England Biolabs
,
M0541
Q5® High-Fidelity 2X Master Mix
,
New England Biolabs
,
M0492
KAPA HiFi HotStart ReadyMix
,
Roche
,KK2602
KOD Hot Start DNA Polymerase
,
Millipore Sigma
,
71086-3
"
E-Gel™ EX Agarose Gels, 1
(18)
Group B Streptococcus Cas9 variants provide insight into programmable gene repression and CRISPR-Cas transcriptional effects
Communications Biology
June 9, 2023
Kathyayini P. Gopalakrishna, Gideon H. Hillebrand, Venkata H. Bhavana, Jordan L. Elder, Adonis D’Mello, Hervé Tettelin, Thomas A. Hooven
ensure silent mutations.
Mutagenesis
PCR
was performed using
NEB
Q5 High Fidelity Polymerase (cat. #
M0492L
).
PCR
products were digested with 1 μL of
DpnI
for 1 h at 37 °C. Samples were then Gibson assembled
(19)
Reduction of Tumor Growth with RNA-Targeting Treatment of the NAB2–STAT6 Fusion Transcript in Solitary Fibrous Tumor Models
Cancers (Basel)
June 9, 2023
Yi Li, John T. Nguyen, Manasvini Ammanamanchi, Zikun Zhou, Elijah F. Harbut, Jose L. Mondaza-Hernandez, Clark A. Meyer, David S. Moura, Javier Martin-Broto, Heather N. Hayenga,
et al.
performed using Q5 High-Fidelity 2× Master Mix (
New England Biolabs
, Ipswich, WA, USA, catalog number:
M0492
). An amount of 100 ng genomic DNAs were used as the template, and the
PCR
conditions were: first, 1
(20)
Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranes
Nature communications
June 9, 2023
Hildebrandt RP, Moss KR, Janusz-Kaminska A, Knudson LA, Denes LT, Saxena T, Boggupalli DP, Li Z, Lin K, Bassell GJ,
et al
.
(cytosolic), and Calnexin (
membrane
) using
qPCR
primers (IDT) with Q5 polymerase (
New England Biolabs
,
M0492
) was measured with a highly sensitive dsDNA detection dye (Lumiprobe, 11010) on a 96-well
RT-PCR
thermal
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Reviews
Write a Review
(1)
8/6/2019
PCR Master Mix for Superior Performance for a Broad Range of Amplicons (NEB)
Atul Singh
It works well for all kinds of PCR.
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Q5 High-Fidelity 2X Master Mix from New England Biolabs
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Pricing Info
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