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Q5 High-Fidelity 2X Master Mix
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Q5 High-Fidelity 2X Master Mix
Q5 High-Fidelity 2X Master Mix from New England Biolabs
(1)
Write a review
Citations:
(273)
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Supplier Page from
New England Biolabs for
Q5 High-Fidelity 2X Master Mix
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Q5 High-Fidelity 2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0492S
Quantity
100 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (273)
(1)
Fucosylated glycoproteins and fucosylated glycolipids play opposing roles in cholera intoxication
bioRxiv
August 3, 2023
Atossa C. Ghorashi, Andrew Boucher, Stephanie A. Archer-Hartmann, Nathan B. Murray, Rohit Sai Reddy Konada, Xunzhi Zhang, Chao Xing, Parastoo Azadi, Ulf Yrlid, Jennifer J. Kohler
amino acid sequence.
Amplification
was done using the
Q5 High-Fidelity 2X Master Mix
(
NEB
, catalog no.
M0492S
) according to manufacturer’s instructions. Amplified ORFs were then purified using the MinElute PCR
(2)
Characterisation of a Live-Attenuated Rabies Virus Expressing a Secreted scFv for the Treatment of Rabies
Viruses
July 31, 2023
Samuel P. Smith, Rebecca Shipley, Pascal Drake, Anthony R. Fooks, Julian Ma, Ashley C. Banyard
08-115
)-coated T75 flasks. 2.2. Molecular Biology
Techniques
PCR
was undertaken using NEB Q5® DNA Polymerase (
M0492L
) according to the manufacturer’s instructions and products isolated using either
NEB
Monarch® DNA Gel Extraction Kit
(3)
Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ
eLife
July 28, 2023
Zhi Yang Tan, Shujun Cai, Alex J Noble, Jon K Chen, Jian Shi, Lu Gan
at the restriction site5’-GATATC-3’ Other,Q5 High-Fidelity 2× Master Mix,
New England BioLabs
,Cat#
M0492
,"Contains Q5 DNA Polymerase, deoxynucleotides and Mg2+ in buffer, for
PCR amplification
" Other,Salmon
(4)
Targeting AAV vectors to the central nervous system by engineering capsid–receptor interactions that enable crossing of the blood–brain barrier
PLOS Biology
July 19, 2023
Qin Huang, Albert T. Chen, Ken Y. Chan, Hikari Sorensen, Andrew J. Barry, Bahar Azari, Qingxia Zheng, Thomas Beddow, Binhui Zhao, Isabelle G. Tobey,
et al.
(CACTCATCGACCAATACTTGTACTATCTCT) as a forward primer in a
PCR
reaction using
Q5 High-Fidelity 2X Master Mix
(
NEB
#
M0492S
) following the manufacturer’s protocol for 30 cycles with 10 ng pUC57-wtAAV9-X/A plasmid. To assemble
(5)
Pleiotropy of autism-associated chromatin regulators
Development
July 15, 2023
Micaela Lasser, Nawei Sun, Yuxiao Xu, Sheng Wang, Sam Drake, Karen Law, Silvano Gonzalez, Belinda Wang, Vanessa Drury, Octavio Castillo,
et al.
individuals with ASD were created by
site-directed mutagenesis
using Q5 High Fidelity Master Mix (
NEB
M0492L
),
T4 polynucleotide kinase
(
NEB
M0201L
) and
T4 DNA ligase
(
NEB
M0202L
). Variants were selected by presence
(6)
Biochemical and structural characterization of the first-discovered metazoan DNA cytosine-N4 methyltransferase from the Bdelloid rotifer Adineta vaga
Journal of Biological Chemistry
July 5, 2023
Jujun Zhou, John R. Horton, Gundeep Kaur, Qin Chen, Xuwen Li, Fabian Mendoza, Tao Wu, Robert M. Blumenthal, Xing Zhang, Xiaodong Cheng
Multiplex
Oligos for Illumina (
NEB
,
E7335S
/
E7500S
/
E7710S
/
E7730S
) and
Q5 High-Fidelity 2X Master Mix
(
NEB
,
M0492S
) (cycle number was determined by
qPCR
with
PowerUP SYBR Green Master Mix
using R1-22nt (5′-CTA CAC GAC
(7)
mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine
Bio-protocol
July 5, 2023
Gao X, Li Y, Nie G, Zhao X.
(
New England Biolabs
, catalog number:
E2060S
)Q5 high-fidelity 2× master mix (
New England Biolabs
, catalog number:
M0492S
)Tris-HCl (1 M, pH 8.0) (Solarbio, catalog number: T1150)MgCl2 (1 M) (Beyotime, catalog number: ST269)Diethylpyrocarbonate
(8)
Assessment of enzymatically synthesized DNA for gene assembly
Frontiers in Bioengineering and Biotechnology
July 5, 2023
Brooke L. Simmons, Nathan D. McDonald, Natalie G. Robinett
incubated at 50°C/30 min. Next, 5 µL of this mixture was added to 12.5 µL of ×2 Q5 Master Mix (
NEB
, #
M0492S
), 2.5 µL each end oligo, and H2O to reach a final concentration of 25 µL. The
PCR
cycles were run as
(9)
Aclarubicin stimulates RNA polymerase II elongation at closely spaced divergent promoters
Science Advances
June 16, 2023
Matthew Wooten, Brittany Takushi, Kami Ahmad, Steven Henikoff
and a uniquely barcoded i7 primer and amplified with
NEB
Q5 high-fidelity 2× master mix (catalog no.
M0492S
). The libraries were purified with 1.1× volume of Sera-Mag carboxylate-modified magnetic beads and subjected
(10)
Modularity and diversity of target selectors in Tn7 transposons
Molecular Cell
June 15, 2023
Guilhem Faure, Makoto Saito, Sean Benler, Iris Peng, Yuri I. Wolf, Jonathan Strecker, Han Altae-Tran, Edwin Neumann, David Li, Kira S. Makarova,
et al.
NEBNext® High-Fidelity 2X PCR Master Mix
,
New England Biolabs
,
M0541
Q5® High-Fidelity 2X Master Mix
,
New England Biolabs
,
M0492
KAPA HiFi HotStart ReadyMix
,
Roche
,KK2602
KOD Hot Start DNA Polymerase
,
Millipore Sigma
,
71086-3
"
E-Gel™ EX Agarose Gels, 1
(11)
Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranes
Nature communications
June 9, 2023
Hildebrandt RP, Moss KR, Janusz-Kaminska A, Knudson LA, Denes LT, Saxena T, Boggupalli DP, Li Z, Lin K, Bassell GJ,
et al
.
(cytosolic), and Calnexin (
membrane
) using
qPCR
primers (IDT) with Q5 polymerase (
New England Biolabs
,
M0492
) was measured with a highly sensitive dsDNA detection dye (Lumiprobe, 11010) on a 96-well
RT-PCR
thermal
(12)
Reduction of Tumor Growth with RNA-Targeting Treatment of the NAB2–STAT6 Fusion Transcript in Solitary Fibrous Tumor Models
Cancers (Basel)
June 9, 2023
Yi Li, John T. Nguyen, Manasvini Ammanamanchi, Zikun Zhou, Elijah F. Harbut, Jose L. Mondaza-Hernandez, Clark A. Meyer, David S. Moura, Javier Martin-Broto, Heather N. Hayenga,
et al.
performed using Q5 High-Fidelity 2× Master Mix (
New England Biolabs
, Ipswich, WA, USA, catalog number:
M0492
). An amount of 100 ng genomic DNAs were used as the template, and the
PCR
conditions were: first, 1
(13)
Group B Streptococcus Cas9 variants provide insight into programmable gene repression and CRISPR-Cas transcriptional effects
Communications Biology
June 9, 2023
Kathyayini P. Gopalakrishna, Gideon H. Hillebrand, Venkata H. Bhavana, Jordan L. Elder, Adonis D’Mello, Hervé Tettelin, Thomas A. Hooven
ensure silent mutations.
Mutagenesis
PCR
was performed using
NEB
Q5 High Fidelity Polymerase (cat. #
M0492L
).
PCR
products were digested with 1 μL of
DpnI
for 1 h at 37 °C. Samples were then Gibson assembled
(14)
Pathogen-specific innate immune response patterns are distinctly affected by genetic diversity
Nature Communications
June 5, 2023
Antje Häder, Sascha Schäuble, Jan Gehlen, Nadja Thielemann, Benedikt C. Buerfent, Vitalia Schüller, Timo Hess, Thomas Wolf, Julia Schröder, Michael Weber,
et al.
ATCATTGAAATTAATTTCACCTGTCCA) with 2x Q5 polymerase master mix according to the manufacturer’s instructions (
NEB
Cat#
M0492L
).
PCR
samples were purified with the
PCR
& Gel
Clean-Up
Kit (Macherey-Nagel Cat# 740609.50) according
(15)
Non-viral nitric oxide-based gene therapy improves perfusion and liposomal doxorubicin sonopermeation in neuroblastoma models
Theranostics
June 4, 2023
Aditi Bellary, Chance Nowak, Isabella Iwanicki, Fernando Flores-Guzman, Lydia Wu, Jessica J. Kandel, Theodore W. Laetsch, Leonidas Bleris, Sonia L. Hernandez, Shashank R. Sirsi
plasmid #19295) 43. The transcript was cloned using
PCR
with
Q5® High-Fidelity DNA Polymerase
(
NEB
, #
M0492S
) and primers P1: cagtagaccggtgagactctggccccacgggacacag and P2: cagtagcaattggaattgtaatacgactcactatagg.
(16)
Identification and heterologous expression of the globomycin biosynthetic gene cluster
Synthetic and Systems Biotechnology
June 1, 2023
Daniel Oves-Costales, Tetiana Gren, Eva Baggesgaard Sterndorff, Jesús Martín, Francisco Javier Ortiz-López, Tue S. Jørgensen, Xinglin Jiang, Fernando Román-Hurtado, Fernando Reyes, Olga Genilloud,
et al.
pL0004. The
PCR
reaction was carried out with the Q5 High Fidelity Master Mix (
New England Biolabs
;
M0492S
), following the manufacturer's instructions. The
spores
for
colony PCR
were harvested from plates using
(17)
Protocol for biallelic tagging of an endogenous gene using CRISPR-Cas9 in human cells
STAR Protocols
June 1, 2023
Nannan Kong, Ying Wai Chan
6023 DNeasy Blood & Tissue Kits,
Qiagen
,Cat#
69504
Q5 High-Fidelity 2× Master Mix,
New England Biolabs
,Cat#
M0492L
FastDigest
Bpil,
Thermo Fisher
,Cat#
FD1014
T7 DNA ligase
,
New England Biolabs
,Cat#
M0318S
BamHI-HF
,
New England Biolabs
(18)
scChIX-seq infers dynamic relationships between histone modifications in single cells
Nature Biotechnology
June 1, 2023
Jake Yeung, Maria Florescu, Peter Zeller, Buys Anton de Barbanson, Max D. Wellenstein, Alexander van Oudenaarden
barcodes and handles was performed by adding 25 μl of
NEBNext Ultra II Q5 Master Mix
(
NEB
, catalog no.
M0492L
), 11 μl nuclease-free water and 2 μl of 10 μM RP1 and RPIx primers.
PCR
protocol for sortChIC experiments Activation
(19)
A noncommutative combinatorial protein logic circuit controls cell orientation in nanoenvironments
Science Advances
May 26, 2023
Jiaxing Chen, Yashavantha L. Vishweshwaraiah, Richard B. Mailman, Erdem D. Tabdanov, Nikolay V. Dokholyan
LOV2. We performed all PCRs using Q5 High-Fidelity Master Mix [New England Biolabs (
NEB
), cat. no.
M0492S
]. After the reaction, we added
DpnI
(
NEB
, cat. no,
R0176S
) enzyme into the
PCR
mixture to digest the
(20)
Reconstruction of a polyclonal ADCC antibody repertoire from an HIV-1 non-transmitting mother
iScience
May 19, 2023
Zak A. Yaffe, Shilei Ding, Kevin Sung, Vrasha Chohan, Lorie Marchitto, Laura Doepker, Duncan Ralph, Ruth Nduati, Frederick A. Matsen, Andrés Finzi,
et al.
proteins","Chemicals, peptides, and recombinant proteins" ,, Q5 High-Fidelity MAster Mix,
New England BioLabs
,Cat#
M0492S
FreeStyle MAX,Thermo Fisher,Cat#16447500 Protein G agarose,
Pierce
,Cat#
20397
293F FreeStyle Expression
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Reviews
Write a Review
(1)
8/6/2019
PCR Master Mix for Superior Performance for a Broad Range of Amplicons (NEB)
Atul Singh
It works well for all kinds of PCR.
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Q5 High-Fidelity 2X Master Mix from New England Biolabs
Supplier Page
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