Q5 High-Fidelity DNA Polymerase from New England Biolabs

Product Specs
ItemQ5 High-Fidelity DNA Polymerase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0491L
Quantity500 units
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeDNA Polymerase
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) The viral fitness and intrinsic pathogenicity of dominant SARS-CoV-2 Omicron sublineages BA.1, BA.2, and BA.5EBioMedicineSeptember 1, 2023Shuai H, Chan JF, Hu B, Chai Y, Yoon C, Liu H, Liu Y, Shi J, Zhu T, Hu JC, et al.Transcriptor First Strand cDNA Synthesis Kit (04897030001, Roche, USA). The viral genome was amplified using Q5® High-Fidelity DNA Polymerase (M0491L, New England Biolabs, USA) with ARTIC network nCoV-2019 primers v4 (Integrated DNA Technologies, USA).

(2) Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopeLife Science AllianceSeptember 1, 2023Li-Chun Cheng, Xi Zhang, Sabyasachi Baboo, Julie A Nguyen, Salvador Martinez-Bartolomé, Esther Loose, Jolene Diedrich, John R Yates, Larry Geracefrom the C3H cells. Genes of interest were amplified by PCR using Q5 high-fidelity DNA polymerase (#M0491L; New England Biolabs). All genes were inserted into either pLV-Ef1a-V5-LIC-IRES-PURO (#120247; Addgene)

(3) Evolution of molecular switches for regulation of transgene expression by clinically licensed gluconateNucleic Acids ResearchAugust 25, 2023Ana Palma Teixeira, Shuai Xue, Jinbo Huang, Martin FusseneggerT4 DNA ligase (EL0011, Thermo Fisher). All PCR reactions were performed using Q5 High-Fidelity DNA polymerase (M0491L, New England Biolabs). After ligation, the plasmids were transformed and amplified in E. coli XL10-Gold

(4) Protocol for bulk and single-nuclei chromatin accessibility quantification in mouse liver tissueSTAR ProtocAugust 16, 2023Noga Korenfeld, Nicolaj I. Toft, Trine V. Dam, Meital Charni-Natan, Lars Grøntved, Ido GoldsteinNuclease-free water,Hylabs,BP731/500D Digitonin,Sigma,D141-100MG Tween-20,Avantor,X251-07 MinElute PCR purification kit,Qiagen,20-28006 Q5 High-Fidelity DNA Polymerase,New England Biolabs,M0491L Q5 Reaction Buffer,New England Biolabs,B9027S PCR dNTP’s Mix,Larova,VAR-02 Agencourt AMPure XP beads,Beckman Coulter

(5) Cold temperature induces a TRPM8-independent calcium release from the endoplasmic reticulum in human plateletsbioRxivJuly 22, 2023Anastasiia Stratiievska, Olga Filippova, Tahsin Özpolat, Daire Byrne, S. Lawrence Lawrence Bailey, Molly Y. Mollica, Jeff Harris, Kali Esancy, Junmei Chen, Ajay K. Dhaka, et al.5’-CAGGCTGAGCGATGAAATGCTGATCTG-3’ PCR amplification was carried out using a Q5® High-Fidelity DNA Polymerase kit (#M0491L, New England Biolabs, Ipswich, MA) using the following protocol: 40 cycles of 30-sec denaturation at

(6) Polycomb repressive complex 2 binds and stabilizes NANOG to suppress differentiation-related genes to promote self-renewaliScienceJuly 21, 2023Yeh DW, Liu C, Hernandez JC, Tahara SM, Tsukamoto H, Machida K.Stellar™ Competent Cells,Takara,636766 Advantage® GC 2 PCR Kit,Takara,639119 Q5® High-Fidelity DNA Polymerase,New England BioLabs,M0491L T4 DNA Ligase,New England BioLabs,M0202L NEBuilder® HiFi DNA Assembly Master Mix,New England BioLabs,E2621L Quick Blunting™ Kit

(7) Systematic characterization of regulatory variants of blood pressure genesCell GenomicsJuly 12, 2023Winona Oliveros, Kate Delfosse, Daniella F. Lato, Katerina Kiriakopulos, Milad Mokhtaridoost, Abdelrahman Said, Brandon J. McMurray, Jared W.L. Browning, Kaia Mattioli, Guoliang Meng, et al.T4 DNA Ligase,NEB,Cat#M0202M XbaI,NEB,Cat#R0145L KpnI-HF,NEB,Cat#R3142L Q5- High Fidelity DNA polymerase,NEB,Cat#M0491L Formamide (Deionized),Invitrogen,Cat#AM9342 Agencourt AMPure XP,Beckman Coulter,Cat#A63881 Trizol Reagent

(8) Low-cost molecular methods to characterise gastrointestinal nematode co-infections of goats in AfricaParasites & VectorsJune 29, 2023Paul M. Airs, Javier Ventura-Cordero, Winchester Mvula, Taro Takahashi, Jan Van Wyk, Patson Nalivata, Andrews Safalaoh, Eric R. Morgannanograms of template DNA was added per 30 μl PCR reaction (Q5® High-Fidelity DNA Polymerase, NEB, cat. M0491L) with a mix of ITS-2 primers containing 0–3 ‘N’ bases and an Illumina adapter (10 μM stock) used at

(9) Genome analysis of Cephalotrichum gorgonifer and identification of the biosynthetic pathway for rasfonin, an inhibitor of KRAS dependent cancerFungal Biology and BiotechnologyJune 24, 2023Andreas Schüller, Lena Studt-Reinhold, Harald Berger, Lucia Silvestrini, Roman Labuda, Ulrich Güldener, Markus Gorfer, Markus Bacher, Maria Doppler, Erika Gasparotto, et al.was either performed with the Q5®-polymerase from New England Biolabs (New England Biolabs; Art. No. M0491L) for plasmid cloning, or with the GoTaq® Green Master Mix (Promega; Madison, WI, USA; Art. No. M7845)

(10) Novel Fas-TNFR chimeras that prevent Fas ligand-mediated kill and signal synergistically to enhance CAR T cell efficacyMolecular Therapy - Nucleic AcidsJune 13, 2023Callum McKenzie, Mohamed El-Kholy, Farhaan Parekh, Mathew Robson, Katarina Lamb, Christopher Allen, James Sillibourne, Shaun Cordoba, Simon Thomas, Martin Pulecodon-optimized open reading frames (GeneArt), were synthesized (IDT) and amplified using Q5 DNA polymerase (NEB, M0491L) and oligonucleotide primers (IDT). The resulting PCR products were fractionated on an agarose gel,

(11) Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxivJune 13, 2023Li-Chun Cheng, Xi Zhang, Sabyasachi Baboo, Julie A Nguyen, Salvador Martinez-Bartolomé, Esther Loose, Jolene Diedrich, John R Yates, Larry GeraceGenes of interest were amplified by PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs #M0491L). All genes were inserted into either pLV-Ef1a-V5-LIC-IRES-PURO (Addgene #120247) or pLV-Ef1a-LIC-V5-IRES-PURO

(12) A protein domain-oriented approach to expand the opportunities of therapeutic exon skipping for USH2A-associated retinitis pigmentosaMolecular therapy. Nucleic acidsJune 13, 2023Schellens RTW, Broekman S, Peters T, Graave P, Malinar L, Venselaar H, Kremer H, De Vrieze E, Van Wijk E.corresponding wild-type zebrafish ush2a alleles. For PCR analysis, the Q5 High-Fidelity DNA Polymerase kit (#M0491L; New England Biolabs, Ipswich, MA, USA) was employed. All primer sequences are listed in Table S2. The

(13) Generation and Characterization of a Zebrafish Model for ADGRV1-Associated Retinal Dysfunction Using CRISPR/Cas9 Genome Editing TechnologyCellsJune 10, 2023Stemerdink M, Broekman S, Peters T, Kremer H, de Vrieze E, van Wijk E.(Applied Biosystems, Waltham, MA, USA) using Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA, #M0491L) and 1× EvaGreen Dye (Biotium, Fremont, CA, USA, #31000). Primers that were used for the PCR amplification

(14) Biochemical Correction of GM2 Ganglioside Accumulation in AB-Variant GM2 GangliosidosisInternational Journal of Molecular SciencesMay 24, 2023Natalie M. Deschenes, Camilyn Cheng, Alex E. Ryckman, Brianna M. Quinville, Prem Khanal, Melissa Mitchell, Zhilin Chen, Waheed Sangrar, Steven J. Gray, Jagdeep S. Waliapunch samples obtained at 21 days of age. Briefly, DNA was digested using Q5® High-Fidelity Master Mix (M0491L; New England BioLabs Ltd., Whitby, ON, Canada) and subsequently analysed by PCR using the following

(15) Structure of Anabaena flos-aquae gas vesicles revealed by cryo-ETStructureMay 4, 2023Przemysław Dutka, Lauren Ann Ann Metskas, Robert C. Hurt, Hossein Salahshoor, Ting-Yu Wang, Dina Malounda, George J. Lu, Tsui-Fen Chou, Mikhail G. Shapiro, Grant J. Jensenproteins","Chemicals, peptides, and recombinant proteins" ,, Q5 High-Fidelity DNA Polymerase,New England Biolabs,Cat# M0491L NEBuilder HiFi DNA Assembly Master Mix,New England Biolabs,Cat# E2621L LB Broth (Lennox),Sigma-Aldrich,Cat#

(16) Multicellular, IVT-derived, unmodified human transcriptome for nanopore direct RNA analysisbioRxivApril 13, 2023Caroline A. McCormick, Stuart Akeson, Sepideh Tavakoli, Dylan Bloch, Isabel N. Klink, Miten Jain, Sara H. RouhanifardThese primers are listed in Supplementary Data 8. The PCR reaction was set up with Q5 polymerase (NEB, M0491L) using the Manufacturer’s protocol. Thermocycling conditions were as follows: initial denaturation at

(17) Initial ciliary assembly in Chlamydomonas requires Arp2/3 complex–dependent endocytosisMolecular Biology of the CellApril 1, 2023Brae M. Bigge, Nicholas E. Rosenthal, Prachee AvasthiARPC4 genetic sequence was isolated from Chlamydomonas cDNA using PCR with the Q5 DNA Polymerase (NEB; M0491L). The resulting fragment and the pChlamy4 plasmid (Thermofisher; A24231) were digested with EcoRI (NEB;

(18) CCR4‐NOT subunit CCF‐1/CNOT7 promotes transcriptional activation to multiple stress responses in Caenorhabditis elegansAging CellApril 1, 2023Hadi Tabarraei, Brandon M. Waddell, Kelly Raymond, Sydney M. Murray, Ying Wang, Keith P. Choe, Cheng‐Wei Wufull‐length ccf‐1 cDNA clone was generated through PCR using the Q5® High‐Fidelity DNA polymerase (M0491L; New England BioLabs) and cloned into the pDEST32 vector containing the GAL4 DNA binding domain (DBD)

(19) Genetic diversity in the metronidazole metabolism genes nitroreductases and pyruvate ferredoxin oxidoreductases in susceptible and refractory clinical samples of Giardia lambliaInternational Journal for Parasitology: Drugs and Drug ResistanceApril 1, 2023Christina S. Saghaug, Astrid L. Gamlem, Kirsti B. Hauge, Juha Vahokoski, Christian Klotz, Toni Aebischer, Nina Langeland, Kurt Hanevikand NR2 genes. All gene-PCRs were carried out using the Q5® High-Fidelity DNA Polymerase (catalog nr M0491L, New England BioLabs (NEB) Ipswich, MA, USA) and reaction setup for 25 μl according to manufacturer.

(20) Characterization of the oral and gut microbiome in children with obesity aged 3 to 5 yearsFrontiers in Cellular and Infection MicrobiologyMarch 29, 2023Ting Ma, Zeyu Wu, Jing Lin, Chao Shan, Aisaiti Abasijiang, Jin ZhaoAmplification of bacterial 16S rRNA was performed using PCR with an NEB Q5 High-Fidelity polymerase (M0491L). Selected primers were F: AGAGTTTGATCMTGGCTCAG; R: ACCTTGTTACGACTT. The target fragment obtained via

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