NEBNext High-Fidelity 2X PCR Master Mix M0541S from New England Biolabs

Product Specs
ItemNEBNext High-Fidelity 2X PCR Master Mix
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0541S
Quantity50 reactions
ApplicationsDNA Analysis, PCR
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeMaster Mix
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Dose rate dependent reduction in chromatin accessibility at transcriptional start sites long time after exposure to gamma radiationEpigeneticsDecember 31, 2023Hildegunn Dahl, Jarle Ballangby, Torstein Tengs, Marcin W. Wojewodzic, Dag M. Eide, Dag Anders Brede, Anne Graupner, Nur Duale, Ann-Karin OlsenµL) was performed using 25 µL 2× NEBnext High-Fidelity PCR Master Mix (New England BioLabs (cat. nr: M0541L), Ipswich, MA, USA) and 2.5 µL forward and reverse Nextera DNA CD indexes (Illumina, San Diego, CA,

(2) Deciphering the mitophagy receptor network identifies a crucial role for OPTN (optineurin) in acute myeloid leukemiaAutophagyNovember 1, 2023Meyer LM, Koschade SE, Vischedyk JB, Thoelken M, Gubas A, Wegner M, Basoglu M, Knapp S, Kaulich M, Eimer S, et al.of each 10 µM PCR1 forward and reverse primers and 25 µL Next High-Fidelity 2× PCR Master Mix (NEB, M0541L) was used. Thermal cycler protocol was applied as follows: Initial denaturation at 98°C for 2 min, 15

(3) The SWI/SNF complex member SMARCB1 supports lineage fidelity in kidney canceriScienceAugust 18, 2023Ludovic Wesolowski, Jianfeng Ge, Leticia Castillon, Debora Sesia, Anna Dyas, Shoko Hirosue, Veronica Caraffini, Anne Y. Warren, Paulo Rodrigues, Giovanni Ciriello, et al.amplified for a total of 8-12 cycles using custom Nextera PCR primers and NEBNext PCR master mix (NEB M0541S). The amplified libraries were purified using Agencourt AMPureXP reagents (A63880), profiled on the

(4) ATRX guards against aberrant differentiation in mesenchymal progenitor cellsbioRxivAugust 8, 2023Yan Fang, Douglas Barrows, Yakshi Dabas, Thomas S Carroll, William D. Tap, Benjamin A. Nacevcollected for Omni-ATAC-seq. Amplification was performed with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541s) and Nextera PCR Primers (8 cycles). The sequences of PCR primers with index adapters are listed in

(5) LRP8‐mediated selenocysteine uptake is a targetable vulnerability in MYCN‐amplified neuroblastomaEMBO Molecular MedicineAugust 7, 2023Hamed Alborzinia, Zhiyi Chen, Umut Yildiz, Florencio Porto Freitas, Felix C E Vogel, Julianna Patricia Varga, Jasmin Batani, Christoph Bartenhagen, Werner Schmitz, Gabriele Büchel, et al.740424). Sequencing libraries were prepared using the NEBNext 2× High‐Fidelity Master Mix (NEB, Cat. No. M0541L) with amplification primers partially complementary to the lentiviral gRNA backbone with overhangs introducing

(6) HDAC1 and HDAC6 are essential for driving growth in IDH1 mutant gliomaSci RepAugust 1, 2023Matthew C. Garrett, Rebecca Albano, Troy Carnwath, Lubayna Elahi, Catherine A. Behrmann, Merissa Pemberton, Daniel Woo, Eric O'Brien, Brett VanCauwenbergh, John Perentesis, et al.Following purification, transposed DNA was mixed with NEBNext high-Fidelity 2× PCR Master Mix (NEB, #M0541S), AD1_noMX and AD2.1–2.16 barcoded primers, and nuclease free water. Samples were amplified for 12 cycles.

(7) The contributions of DNA accessibility and transcription factor occupancy to enhancer activity during cellular differentiationbioRxivJuly 27, 2023Trevor Long, Tapas Bhattacharyya, Andrea Repele, Madison Naylor, Sunil Nooti, Shawn Krueger, Manuamplified in a 50μL PCR reaction using NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs, M0541S) for 5 cycles after a 5 min elongation step. Primers included adaptors for sequencing and were barcoded

(8) FOXA2 controls the anti-oxidant response in FH-deficient cellsCell ReportsJuly 25, 2023Connor Rogerson, Marco Sciacovelli, Lucas A. Maddalena, Andromachi Pouikli, Marc Segarra-Mondejar, Lorea Valcarcel-Jimenez, Christina Schmidt, Ming Yang, Elena Ivanova, Joshua Kent, et al.HiFi Hot Start DNA polymerase,KAPA Biosystems,KK2500 NEBNext® High-Fidelity 2X PCR Master Mix,NEB,M0541S Ampure XP beads,Beckman Coulter,A63880 5x siRNA buffer,Horizon,B-002000-UB-100 Lipofectamine RNAiMAX,ThermoFisher,13778075 Revert™

(9) Proximity-dependent recruitment of Polycomb repressive complexes by the lncRNA AirnCell reportsJuly 25, 2023Braceros AK, Schertzer MD, Omer A, Trotman JB, Davis ES, Dowen JM, Phanstiel DH, Aiden EL, Calabrese JM.ligation by Quick ligase (NEB, cat #: M2200S), and NEBNext High-Fidelity 2X PCR Master Mix (NEB, cat #: M0541S). All DNA size-selection purification steps were performed with AMPure XP beads (Beckman Coulter, cat

(10) Coordinated inheritance of extrachromosomal DNA species in human cancer cellsbioRxivJuly 25, 2023King L. Hung, Matthew G. Jones, Ivy Tsz-Lo Wong, Joshua T. Lange, Jens Luebeck, Elisa Scanu, Britney Jiayu He, Lotte Brückner, Rui Li, Rocío Chamorro González, et al.were generated by 5–7 rounds of PCR amplification using NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541L), purified using SPRIselect reagent kit (Beckman Coulter, B23317) with double size selection (0.8× right,

(11) Systematic mapping and modeling of 3D enhancer-promoter interactions in early mouse embryonic lineages reveal regulatory principles that determine the levels and cell-type specificity of gene expressionbioRxivJuly 25, 2023Dylan Murphy, Eralda Salataj, Dafne Campigli Di Giammartino, Javier Rodriguez-Hernaez, Andreas Kloetgen, Vidur Garg, Erin Char, Christopher M. Uyehara, Ly-sha Ee, UkJin Lee, et al.Kit (D4014). ATAC-seq libraries were prepared using NEBNext High-Fidelity 2X PCR Master Mix (NEB, #M0541), a uniquely barcoded primer per sample, and a universal primer. Samples were first subjected to 5 cycles

(12) The BAF chromatin complex component SMARCC1 does not mediate GLI transcriptional repression of Hedgehog target genes in limb budsbioRxivJuly 21, 2023Janani Ramachandran, Wanlu Chen, Rachel K. Lex, Kathryn E. Windsor, Hyunji Lee, Tingchang Wang, Weiqiang Zhou, Hongkai Ji, Steven A. Vokes37°C, shaking gently. Libraries were generated using the NEB High-Fidelity 2x Mix (New England Biolabs M0541S), PCR amplified for 11 cycles and cleaned up using SparQ PureMag beads (QuantaBio 95196-060). Libraries

(13) De novo purine metabolism is a metabolic vulnerability of cancers with low p16 expressionbioRxivJuly 20, 2023Naveen Kumar Tangudu, Raquel Buj, Jiefei Wang, Aidan R. Cole, Apoorva Uboveja, Richard Fang, Amandine Amalric, Maureen A. Lyons, Uma R. Chandran, Katherine M. AirdAddgene 52961) by PCR amplification using NEB Next High-Fidelity PCR Master Mix (New England Biolabs, M0541S). The PCR product was digested with Esp3I (BsmBI) (Fisher Scientific, FERFD0454) and ligated into the

(14) High throughput PRIME editing screens identify functional DNA variants in the human genomebioRxivJuly 18, 2023Xingjie Ren, Han Yang, Jovia L. Nierenberg, Yifan Sun, Jiawen Chen, Cooper Beaman, Thu Pham, Mai Nobuhara, Maya Asami Takagi, Vivek Narayan, et al.were performed using 0.01 fmol of pooled oligos with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541S). Each 20 μl PCR mix was combined with 40 μl of oil-surfactant mixture (containing 4.5 % Span 80 (v/v),

(15) Genome-wide CRISPR screens define determinants of epithelial-mesenchymal transition mediated immune evasion by pancreatic cancer cellsScience AdvancesJuly 14, 2023Yuanzhuo Gu, Zhengkui Zhang, Marcel G. M. Camps, Ferry Ossendorp, Ruud H. Wijdeven, Peter ten Dijke(51194, Qiagen). sgRNA sequences were recovered by PCR using NEBNext High-Fidelity 2x PCR Master Mix (M0541L, New England BioLabs) with primers as described in https://addgene.org/browse/article/16517/. Sequencing

(16) Relaxed targeting rules help PIWI proteins silence transposonsNatureJuly 13, 2023Ildar Gainetdinov, Joel Vega-Badillo, Katharine Cecchini, Ayca Bagci, Cansu Colpan, Dipayan De, Shannon Bailey, Amena Arif, Pei-Hsuan Wu, Ian J. MacRae, et al.Ampure XP beads (Beckman Coulter, A63880), cDNA was PCR amplified using NEBNext High-Fidelity (NEB, M0541; 98 °C for 30 s; 4 cycles of 98 °C for 10 s, 59 °C for 30 s, 72 °C for 12 s; 6 cycles of 98 °C for 10

(17) The Mediator complex regulates enhancer-promoter interactionsNature Structural & Molecular BiologyJuly 10, 2023Shyam Ramasamy, Abrar Aljahani, Magdalena A. Karpinska, T. B. Ngoc Cao, Taras Velychko, J. Neos Cruz, Michael Lidschreiber, A. Marieke Oudelaarsamples were amplified using Nextera indexing primers and NEBNext High-Fidelity PCR Master Mix (NEB, M0541), with an initial 5-min extension step at 72 °C. A real-time PCR library amplification kit (KAPA, KK2701)

(18) PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infectionNatureJuly 1, 2023Xu D, Jiang W, Wu L, Gaudet RG, Park ES, Su M, Cheppali SK, Cheemarla NR, Kumar P, Uchil PD, et al.Protease Inhibitor Cocktail (11697498001) was obtained from Roche. High-Fidelity 2X PCR Master Mix (M0541L) was obtained from NEB. Plasmid constructs The following constructs were obtained from Addgene: pLenti-hACE2-hygro

(19) Fanzor is a eukaryotic programmable RNA-guided endonucleaseNatureJune 28, 2023Makoto Saito, Peiyu Xu, Guilhem Faure, Samantha Maguire, Soumya Kannan, Han Altae-Tran, Sam Vo, AnAn Desimone, Rhiannon K. Macrae, Feng Zhang12.5 µl PCR reaction. Target sites were amplified with NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L) under the following thermal cycling conditions: one cycle, 98 °C, 30 s; 15 cycles, 98 °C, 10 s, 65

(20) Selective binding of retrotransposons by ZFP352 facilitates the timely dissolution of totipotency networkNature CommunicationsJune 20, 2023Zhengyi Li, Haiyan Xu, Jiaqun Li, Xiao Xu, Junjiao Wang, Danya Wu, Jiateng Zhang, Juan Liu, Ziwei Xue, Guankai Zhan, et al.using MinElute PCR Purification Kit (QIAGEN, 28006), and amplified using 1xNEBnext PCR master mix (NEB, M0541S) using custom Nextera PCR primers 1 and 2. Upon purification with MinElute PCR Purification Kit (QIAGEN,

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NEBNext High-Fidelity 2X PCR Master Mix

NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs

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NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs

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