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NEBNext High-Fidelity 2X PCR Master Mix
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NEBNext High-Fidelity 2X PCR Master Mix
NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs
Be the first to write a review!
Citations:
(451)
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Supplier Page from
New England Biolabs for
NEBNext High-Fidelity 2X PCR Master Mix
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
NEBNext High-Fidelity 2X PCR Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0541S
Quantity
50 reactions
Applications
DNA Analysis, PCR
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (451)
(1)
Dose rate dependent reduction in chromatin accessibility at transcriptional start sites long time after exposure to gamma radiation
Epigenetics
December 31, 2023
Hildegunn Dahl, Jarle Ballangby, Torstein Tengs, Marcin W. Wojewodzic, Dag M. Eide, Dag Anders Brede, Anne Graupner, Nur Duale, Ann-Karin Olsen
µL) was performed using 25 µL 2× NEBnext High-Fidelity PCR Master Mix (
New England BioLabs
(cat. nr:
M0541L
), Ipswich, MA, USA) and 2.5 µL forward and reverse Nextera DNA CD indexes (Illumina, San Diego, CA,
(2)
Deciphering the mitophagy receptor network identifies a crucial role for OPTN (optineurin) in acute myeloid leukemia
Autophagy
November 1, 2023
Meyer LM, Koschade SE, Vischedyk JB, Thoelken M, Gubas A, Wegner M, Basoglu M, Knapp S, Kaulich M, Eimer S,
et al
.
of each 10 µM PCR1 forward and reverse primers and 25 µL Next High-Fidelity 2× PCR Master Mix (
NEB
,
M0541L
) was used. Thermal cycler protocol was applied as follows: Initial denaturation at 98°C for 2 min, 15
(3)
The SWI/SNF complex member SMARCB1 supports lineage fidelity in kidney cancer
iScience
August 18, 2023
Ludovic Wesolowski, Jianfeng Ge, Leticia Castillon, Debora Sesia, Anna Dyas, Shoko Hirosue, Veronica Caraffini, Anne Y. Warren, Paulo Rodrigues, Giovanni Ciriello,
et al.
amplified for a total of 8-12 cycles using custom Nextera
PCR
primers and NEBNext PCR master mix (
NEB
M0541S
). The amplified libraries were purified using Agencourt AMPureXP reagents (A63880), profiled on the
(4)
ATRX guards against aberrant differentiation in mesenchymal progenitor cells
bioRxiv
August 8, 2023
Yan Fang, Douglas Barrows, Yakshi Dabas, Thomas S Carroll, William D. Tap, Benjamin A. Nacev
collected for Omni-
ATAC-seq
.
Amplification
was performed with NEBNext High-Fidelity 2× PCR Master Mix (
NEB
,
M0541s
) and Nextera
PCR
Primers (8 cycles). The sequences of
PCR
primers with index adapters are listed in
(5)
LRP8‐mediated selenocysteine uptake is a targetable vulnerability in MYCN‐amplified neuroblastoma
EMBO Molecular Medicine
August 7, 2023
Hamed Alborzinia, Zhiyi Chen, Umut Yildiz, Florencio Porto Freitas, Felix C E Vogel, Julianna Patricia Varga, Jasmin Batani, Christoph Bartenhagen, Werner Schmitz, Gabriele Büchel,
et al.
740424).
Sequencing
libraries were prepared using the NEBNext 2× High‐Fidelity Master Mix (
NEB
, Cat. No.
M0541L
) with
amplification
primers partially complementary to the lentiviral gRNA backbone with overhangs introducing
(6)
HDAC1 and HDAC6 are essential for driving growth in IDH1 mutant glioma
Sci Rep
August 1, 2023
Matthew C. Garrett, Rebecca Albano, Troy Carnwath, Lubayna Elahi, Catherine A. Behrmann, Merissa Pemberton, Daniel Woo, Eric O'Brien, Brett VanCauwenbergh, John Perentesis,
et al.
Following
purification
, transposed DNA was mixed with NEBNext high-Fidelity 2× PCR Master Mix (
NEB
, #
M0541S
), AD1_noMX and AD2.1–2.16 barcoded primers, and nuclease free water. Samples were amplified for 12 cycles.
(7)
The contributions of DNA accessibility and transcription factor occupancy to enhancer activity during cellular differentiation
bioRxiv
July 27, 2023
Trevor Long, Tapas Bhattacharyya, Andrea Repele, Madison Naylor, Sunil Nooti, Shawn Krueger, Manu
amplified in a 50μL
PCR
reaction using
NEBNext High-Fidelity 2x PCR Master Mix
(
New England BioLabs
,
M0541S
) for 5 cycles after a 5 min elongation step. Primers included adaptors for
sequencing
and were barcoded
(8)
FOXA2 controls the anti-oxidant response in FH-deficient cells
Cell Reports
July 25, 2023
Connor Rogerson, Marco Sciacovelli, Lucas A. Maddalena, Andromachi Pouikli, Marc Segarra-Mondejar, Lorea Valcarcel-Jimenez, Christina Schmidt, Ming Yang, Elena Ivanova, Joshua Kent,
et al.
HiFi Hot Start DNA polymerase,KAPA Biosystems,KK2500
NEBNext® High-Fidelity 2X PCR Master Mix
,
NEB
,
M0541S
Ampure XP beads,
Beckman Coulter
,
A63880
5x siRNA buffer,
Horizon
,
B-002000-UB-100
Lipofectamine RNAiMAX,
ThermoFisher
,
13778075
Revert™
(9)
Proximity-dependent recruitment of Polycomb repressive complexes by the lncRNA Airn
Cell reports
July 25, 2023
Braceros AK, Schertzer MD, Omer A, Trotman JB, Davis ES, Dowen JM, Phanstiel DH, Aiden EL, Calabrese JM.
ligation
by Quick ligase (
NEB
, cat #:
M2200S
), and
NEBNext High-Fidelity 2X PCR Master Mix
(
NEB
, cat #:
M0541S
). All DNA size-selection
purification
steps were performed with AMPure XP beads (
Beckman Coulter
, cat
(10)
Coordinated inheritance of extrachromosomal DNA species in human cancer cells
bioRxiv
July 25, 2023
King L. Hung, Matthew G. Jones, Ivy Tsz-Lo Wong, Joshua T. Lange, Jens Luebeck, Elisa Scanu, Britney Jiayu He, Lotte Brückner, Rui Li, Rocío Chamorro González,
et al.
were generated by 5–7 rounds of
PCR amplification
using NEBNext High-Fidelity 2× PCR Master Mix (
NEB
,
M0541L
), purified using SPRIselect reagent kit (Beckman Coulter, B23317) with double size selection (0.8× right,
(11)
Systematic mapping and modeling of 3D enhancer-promoter interactions in early mouse embryonic lineages reveal regulatory principles that determine the levels and cell-type specificity of gene expression
bioRxiv
July 25, 2023
Dylan Murphy, Eralda Salataj, Dafne Campigli Di Giammartino, Javier Rodriguez-Hernaez, Andreas Kloetgen, Vidur Garg, Erin Char, Christopher M. Uyehara, Ly-sha Ee, UkJin Lee,
et al.
Kit (D4014).
ATAC-seq
libraries were prepared using
NEBNext High-Fidelity 2X PCR Master Mix
(
NEB
, #
M0541
), a uniquely barcoded primer per sample, and a universal primer. Samples were first subjected to 5 cycles
(12)
The BAF chromatin complex component SMARCC1 does not mediate GLI transcriptional repression of Hedgehog target genes in limb buds
bioRxiv
July 21, 2023
Janani Ramachandran, Wanlu Chen, Rachel K. Lex, Kathryn E. Windsor, Hyunji Lee, Tingchang Wang, Weiqiang Zhou, Hongkai Ji, Steven A. Vokes
37°C, shaking gently. Libraries were generated using the NEB High-Fidelity 2x Mix (
New England Biolabs
M0541S
),
PCR
amplified for 11 cycles and cleaned up using SparQ PureMag beads (QuantaBio 95196-060). Libraries
(13)
De novo purine metabolism is a metabolic vulnerability of cancers with low p16 expression
bioRxiv
July 20, 2023
Naveen Kumar Tangudu, Raquel Buj, Jiefei Wang, Aidan R. Cole, Apoorva Uboveja, Richard Fang, Amandine Amalric, Maureen A. Lyons, Uma R. Chandran, Katherine M. Aird
Addgene 52961) by
PCR amplification
using NEB Next High-Fidelity PCR Master Mix (
New England Biolabs
,
M0541S
). The
PCR product
was digested with
Esp3I
(
BsmBI
) (Fisher Scientific, FERFD0454) and ligated into the
(14)
High throughput PRIME editing screens identify functional DNA variants in the human genome
bioRxiv
July 18, 2023
Xingjie Ren, Han Yang, Jovia L. Nierenberg, Yifan Sun, Jiawen Chen, Cooper Beaman, Thu Pham, Mai Nobuhara, Maya Asami Takagi, Vivek Narayan,
et al.
were performed using 0.01 fmol of pooled oligos with NEBNext High-Fidelity 2× PCR Master Mix (
NEB
,
M0541S
). Each 20 μl PCR mix was combined with 40 μl of oil-surfactant mixture (containing 4.5 % Span 80 (v/v),
(15)
Genome-wide CRISPR screens define determinants of epithelial-mesenchymal transition mediated immune evasion by pancreatic cancer cells
Science Advances
July 14, 2023
Yuanzhuo Gu, Zhengkui Zhang, Marcel G. M. Camps, Ferry Ossendorp, Ruud H. Wijdeven, Peter ten Dijke
(
51194
,
Qiagen
). sgRNA sequences were recovered by
PCR
using
NEBNext High-Fidelity 2x PCR Master Mix
(
M0541L
,
New England BioLabs
) with primers as described in https://addgene.org/browse/article/16517/.
Sequencing
(16)
Relaxed targeting rules help PIWI proteins silence transposons
Nature
July 13, 2023
Ildar Gainetdinov, Joel Vega-Badillo, Katharine Cecchini, Ayca Bagci, Cansu Colpan, Dipayan De, Shannon Bailey, Amena Arif, Pei-Hsuan Wu, Ian J. MacRae,
et al.
Ampure XP beads (
Beckman Coulter
,
A63880
), cDNA was
PCR
amplified using NEBNext High-Fidelity (
NEB
,
M0541
; 98 °C for 30 s; 4 cycles of 98 °C for 10 s, 59 °C for 30 s, 72 °C for 12 s; 6 cycles of 98 °C for 10
(17)
The Mediator complex regulates enhancer-promoter interactions
Nature Structural & Molecular Biology
July 10, 2023
Shyam Ramasamy, Abrar Aljahani, Magdalena A. Karpinska, T. B. Ngoc Cao, Taras Velychko, J. Neos Cruz, Michael Lidschreiber, A. Marieke Oudelaar
samples were amplified using Nextera indexing primers and NEBNext High-Fidelity PCR Master Mix (
NEB
,
M0541
), with an initial 5-min extension step at 72 °C. A
real-time PCR
library amplification
kit (
KAPA
,
KK2701
)
(18)
PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection
Nature
July 1, 2023
Xu D, Jiang W, Wu L, Gaudet RG, Park ES, Su M, Cheppali SK, Cheemarla NR, Kumar P, Uchil PD,
et al
.
Protease Inhibitor Cocktail (11697498001) was obtained from Roche. High-Fidelity 2X PCR Master Mix (
M0541L
) was obtained from
NEB
. Plasmid constructs The following constructs were obtained from Addgene: pLenti-hACE2-hygro
(19)
Fanzor is a eukaryotic programmable RNA-guided endonuclease
Nature
June 28, 2023
Makoto Saito, Peiyu Xu, Guilhem Faure, Samantha Maguire, Soumya Kannan, Han Altae-Tran, Sam Vo, AnAn Desimone, Rhiannon K. Macrae, Feng Zhang
12.5 µl
PCR
reaction. Target sites were amplified with
NEBNext High-Fidelity 2X PCR Master Mix
(
NEB
,
M0541L
) under the following thermal cycling conditions: one cycle, 98 °C, 30 s; 15 cycles, 98 °C, 10 s, 65
(20)
Selective binding of retrotransposons by ZFP352 facilitates the timely dissolution of totipotency network
Nature Communications
June 20, 2023
Zhengyi Li, Haiyan Xu, Jiaqun Li, Xiao Xu, Junjiao Wang, Danya Wu, Jiateng Zhang, Juan Liu, Ziwei Xue, Guankai Zhan,
et al.
using MinElute PCR Purification Kit (
QIAGEN
,
28006
), and amplified using 1xNEBnext PCR master mix (
NEB
,
M0541S
) using custom Nextera
PCR
primers 1 and 2. Upon
purification
with MinElute PCR Purification Kit (QIAGEN,
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NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs
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Pricing Info
Product Specs
Company Info
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